EP0795601A2
Expired / Lapsed
EP
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Biosensor
Assignee: Panasonic Holdings Corp
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Filing: 1997-03-03
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Granted: —
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Expires: 2017-03-03
Abstract
Abstract The biosensor comprises an electrically insulating base plate (1), an electrode system including a working electrode (4) and a counter electrode (5) provided on the base plate, and a reaction layer (7) containing a hydrophilic polymer, an enzyme and an electron acceptor located over the electrode system. The reaction layer (7) contains an excess amount of hydrophilic polymer in a range of 150 to 1,000% by weight of the enzyme, so that the biosensor is free from the effect of any components which are in a sample solution in addition to a substrate. <IMAGE>
Inventors
- 👤 Yoshiko Miyamoto
- 👤 Toshihiko Yoshioka
- 👤 Shiro Nankai
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-30 08:50
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
63 words
A biosensor comprising: an electrically insulating base plate, an electrode system including a working electrode and a counter electrode provided on said base plate, and a reaction layer containing a hydrophilic polymer, an enzyme and an electron acceptor formed over said electrode system, wherein said reaction layer contains said hydrophilic polymer in a range of 150 to 1,000% by weight of said enzyme.
Claim 2
Dependent Claim
20 words
The biosensor in accordance with claim 1, wherein the content of said enzyme is 0.5 to 50µg per sensor tip.
Claim 3
Dependent Claim
27 words
The biosensor in accordance with claim 1, wherein said hydrophilic polymer is selected from carboxymethyl cellulose, carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxyethylmethyl cellulose, amylose, and starch.
Claim 4
Dependent Claim
40 words
The biosensor in accordance with claim 1, wherein said enzyme is at least one selected from glucose oxidase, glucose dehydrogenase, alcohol oxidase, alcohol dehydrogenase, cholesterol oxidase, cholesterol dehydrogenase, lactate oxidase, lactate dehydrogenase, fructose dehydrogenase, ascorbic acid oxidase, and bilirubin oxidase.
Claim 5
Dependent Claim
33 words
The biosensor in accordance with claim 1, wherein said reaction layer is composed of a first layer containing the hydrophilic polymer and a second layer containing the hydrophilic polymer, enzyme and electron acceptor.
Claim 6
Dependent Claim
33 words
The biosensor in accordance with claim 5, wherein the hydrophilic polymer included in the first layer is at least one selected from carboxymethyl cellulose, carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, and carboxyethylmethyl cellulose.
References Cited (Backward Citations)
No citation records indexed.
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