Biosensor containing an enzyme and a sugar

Abstract

Abstract A biosensor which enables rapid and simple quantitation of a substrate with high accuracy and which demonstrates an excellent preservation characteristic by best retaining enzyme activity is disclosed. The biosensor comprises an electrically insulating base plate, an electrode system including at least a working electrode and a counter electrode formed on the base plate, and a reaction layer containing at least an enzyme and a sugar formed on the electrode system, wherein the enzyme is at least one selected from the group consisting of glucose oxidase, glucose dehydrogenase and fructose dehydrogenase and the sugar is at least one selected from the group consisting of trehalose, sucrose, glycerol, mannitol and ribose. When the enzyme is glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone, the use of trehalose as sugar is particularly preferable.

Inventors

• 👤 Keiko Yugawa
• 👤 Toshihiko Yoshioka
• 👤 Shiro Nankai

Bibliographic Details