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US6573099B2 Expired / Lapsed US ⏱️ 0.0 years remaining

Genetic constructs for delaying or repressing the expression of a target gene

Assignee: Commonwealth Scientific and Industrial Research Organisation
•
Filing: 1998-06-19
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Granted: 2003-06-03
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Expires: 2018-06-19
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Abstract

Abstract The present invention relates generally to synthetic genes for modifying endogenous gene expression in a cell, tissue or organ of a transgenic organism, in particular a transgenic animal or plant. More particularly, the present invention provides novel synthetic genes and genetic constructs which are capable of repressing delaying or otherwise reducing the expression of an endogenous gene or a target gene in an organism when introduced thereto.

Inventors

  • 👤 Michael Wayne Graham

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-05 07:04
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/11; C12N15/113; C12N15/67; C12N15/69; C12N15/82; C12N15/8216; C12N15/8218; C12N15/8241; C12N15/8261; C12N15/8271; C12N15/8279; C12N15/8283; C12N9/00; C12N9/0004; C12N9/0071; C12N9/10; C12N9/1048; C12N9/1051; C12N9/12; C12N9/1241; C12N9/127; C12N9/14; C12N9/48; C12N9/50; C12N9/503; C12N2800/00; C12N2800/10; C12N2800/108; C12N2830/00; C12N2830/001; C12N2830/002; C12N2830/15; C12N2830/38; C12N2830/42; C12N2830/55; C12N2830/60; C12N2840/00; C12N2840/10; C12N2840/102; C12N2840/20

Claims Analysis

Total Claims: 29
Claim 1
Independent Claim 119 words
An isolated genetic construct which is capable of delaying, repressing or otherwise reducing the expression of a target gene in an animal cell which is transfected with said genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence, wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, and wherein said at least two copies of said structural gene sequence are placed operably under the control of a single promoter sequence which is operable in said cell, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of said promoter sequence.
Claim 2
Dependent Claim 119 words
An isolated genetic construct which is capable of delaying, repressing or otherwise reducing the expression of a target gene in an animal cell which is transfected with said genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence, wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, and wherein said at least two copies of said structural gene sequence are placed operably under the control of a single promoter sequence which is operable in said cell, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of said promoter sequence.
Claim 3
Dependent Claim 115 words
An isolated genetic construct which is capable of delaying, repressing or otherwise reducing the expression of a target gene in an animal cell which is transfected with said genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence wherein each copy of said structural gene sequence is separately placed under the control of a promoter which is operable in said cell, and wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of an individual promoter sequence.
Claim 4
Dependent Claim 115 words
An isolated genetic construct which is capable of delaying, repressing or otherwise reducing the expression of a target gene in an animal cell which is transfected with said genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence wherein each copy of said structural gene sequence is separately placed under the control of a promoter which is operable in said cell, and wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of an individual promoter sequence.
Claim 5
Dependent Claim 145 words
An isolated genetic construct which is capable of delaying, repressing or otherwise reducing the expression of a target gene in an animal cell which is transfected with said genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence, wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, and wherein said at least two copies of said structural gene sequence are placed operably under the control of a single promoter sequence which is operable in said cell, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of said promoter sequence and wherein at least one other copy of said structural gene sequence is placed operably in the antisense orientation under the control of said promoter sequence.
Claim 6
Dependent Claim 145 words
An isolated genetic construct which is capable of delaying, repressing or otherwise reducing the expression of a target gene in an animal cell which is transfected with said genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence, wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, and wherein said at least two copies of said structural gene sequence are placed operably under the control of a single promoter sequence which is operable in said cell, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of said promoter sequence and wherein at least one other copy of said structural gene sequence is placed operably in the antisense orientation under the control of said promoter sequence.
Claim 7
Dependent Claim 142 words
An isolated genetic construct which is capable of delaying, repressing or otherwise reducing the expression of a target gene in an animal cell which is transfected with said genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence and each copy of said structural gene sequence is separately placed under the control of a promoter which is operable in said cell, and wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of an individual promoter sequence, and wherein at least one other copy of said structural gene sequence is placed operably in the antisense orientation under the control of another individual promoter sequence.
Claim 8
Dependent Claim 142 words
An isolated genetic construct which is capable of delaying, repressing or otherwise reducing the expression of a target gene in an animal cell which is transfected with said genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence and each copy of said structural gene sequence is separately placed under the control of a promoter which is operable in said cell, and wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of an individual promoter sequence, and wherein at least one other copy of said structural gene sequence is placed operably in the antisense orientation under the control of another individual promoter sequence.
Claim 9
Dependent Claim 200 words
An isolated genetic construct which is capable of delaying, repressing or otherwise reducing the expression of a target gene in an animal cell which is transfected with said genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence, wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, and wherein said at least two copies of said structural gene sequence are placed operably under the control of a single promoter sequence which is operable in said cell, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of said promoter sequence, wherein at least one other copy of said structural gene sequence is placed operably in the antisense orientation under the control of said promoter sequence, and wherein said at least one copy of said structural gene sequence that is placed in the sense orientation relative to said promoter and said at least one copy of said structural gene sequence that is placed in the antisense orientation relative to said promoter are spaced from each other by a nucleic acid stuffer fragment.
Claim 10
Dependent Claim 200 words
An isolated genetic construct which is capable of delaying, repressing or otherwise reducing the expression of a target gene in an animal cell which is transfected with said genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence, wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, and wherein said at least two copies of said structural gene sequence are placed operably under the control of a single promoter sequence which is operable in said cell, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of said promoter sequence, wherein at least one other copy of said structural gene sequence is placed operably in the antisense orientation under the control of said promoter sequence, and wherein said at least one copy of said structural gene sequence that is placed in the sense orientation relative to said promoter and said at least one copy of said structural gene sequence that is placed in the antisense orientation relative to said promoter are spaced from each other by a nucleic acid stuffer fragment.
Claim 11
Dependent Claim 20 words
An animal cell comprising the genetic construct of any one of claims 1 - 2 or 3 - 5 .
Claim 12
Independent Claim 114 words
A method of delaying or repressing the expression of a target gene in an animal cell, comprising transfecting said animal cell with a genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence, wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, and wherein said at least two copies of said structural gene sequence are placed operably under the control of a single promoter sequence which is operable in said cell, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of said promoter sequence.
Claim 13
Independent Claim 114 words
A method of delaying or repressing the expression of a target gene in an animal cell, comprising transfecting said animal cell with a genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence, wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, and wherein said at least two copies of said structural gene sequence are placed operably under the control of a single promoter sequence which is operable in said cell, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of said promoter sequence.
Claim 14
Dependent Claim 32 words
The method according to claim 7 , wherein at least one other copy of said structural gene sequence is placed operably in the antisense orientation under the control of said promoter sequence.
Claim 15
Dependent Claim 56 words
The method according to claim 8 , wherein said copy of said structural gene sequence that is placed in the sense orientation relative to said promoter and said copy of said structural gene sequence that is placed in the antisense orientation relative to said promoter are spaced from each other by a nucleic acid stuffer fragment.
Claim 16
Independent Claim 110 words
A method of delaying or repressing the expression of a target gene in an animal cell, comprising expressing in said animal cell a genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence, wherein each copy of said structural gene sequence is separately placed under the control of a promoter which is operable in said cell, and wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of an individual promoter sequence.
Claim 17
Independent Claim 110 words
A method of delaying or repressing the expression of a target gene in an animal cell, comprising expressing in said animal cell a genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence, wherein each copy of said structural gene sequence is separately placed under the control of a promoter which is operable in said cell, and wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of an individual promoter sequence.
Claim 18
Dependent Claim 30 words
The isolated genetic construct according to any one of claims 1 , 2 or 3 - 5 wherein said region of the target gene is 20 to 30 nucleotides long.
Claim 19
Independent Claim 26 words
The method according to any one of claims 7 - 9 or 10 wherein said region of the target gene is 20 to 30 nucleotides long.
Claim 20
Dependent Claim 26 words
The isolated genetic construct according to any one of claims 1 , 2 or 3 - 5 , comprising two copies of said structural gene sequence.
Claim 21
Dependent Claim 30 words
The isolated genetic construct according to any one of claims 1 , 2 or 3 - 5 wherein said region of the target gene is at least 30 nucleotides long.
Claim 22
Dependent Claim 36 words
The isolated genetic construct according to any one of claims 1 , 2 or 3 - 5 wherein said structural gene sequence comprises a nucleotide sequence that is identical to said region of said target gene.
Claim 23
Independent Claim 26 words
The method according to any one of claims 7 - 9 or 10 , wherein said genetic construct comprises two copies of said structural gene sequence.
Claim 24
Independent Claim 27 words
The method according to any one of claims 7 - 9 or 10 , wherein said region of the target gene is at least 30 nucleotides long.
Claim 25
Independent Claim 33 words
The method according to any one of claims 7 - 9 or 10 , wherein said structural gene sequence comprises a nucleotide sequence that is identical to said region of said target gene.
Claim 26
Dependent Claim 12 words
An animal cell comprising the genetic construct according to claim 11 .
Claim 27
Dependent Claim 12 words
An animal cell comprising the genetic construct according to claim 13 .
Claim 28
Dependent Claim 12 words
An animal cell comprising the genetic construct according to claim 14 .
Claim 29
Dependent Claim 12 words
An animal cell comprising the genetic construct according to claim 15 .

References Cited (Backward Citations)

EP0242016A1 Cited
WO1990011682A1 Cited
US5034323A Cited
US5231020A Cited
US5283184A Cited
US5714323A Cited
US5583021A Cited
EP0560156A2 Cited
WO1993023551A1 Cited
WO1994017194A1 Cited
WO1995010607A1 Cited
US5686649A Cited
WO1996008558A1 Cited
WO1997001952A1 Cited
WO1998036083A1 Cited
WO1998037213A1 Cited
WO1998044138A1 Cited
WO1999015682A2 Cited
WO1999025853A1 Cited
WO1999032619A1 Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 2006-06-02 2006-12-02 2007-06-03 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 2010-06-02 2010-12-02 2011-06-03 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2014-06-02 2014-12-02 2015-06-03 $7700.00 Window Closed / Lapsed

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