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US5686649A
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Suppression of plant gene expression using processing-defective RNA constructs
Assignee: Rockefeller University
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Filing: 1995-01-19
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Granted: 1997-11-11
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Expires: 2015-01-19
Abstract
Abstract Disclosed is a novel method of suppressing plant gene expression. The suppression is achieved by transforming a plant with a DNA construct encoding a processing-defective RNA (pd-RNA constructs). A pd-RNA construct comprises a plant active promoter operably linked to a pd-RNA encoding segment (pd-RNA segment), wherein the pd-RNA segment comprises a sequence substantially homologous to the target gene and a defective intron and/or a defective 3' termination and processing sequence (hereinafter 3' processing sequence). The pd-RNA constructs of the present invention are designed to express target-gene-homologous RNA transcripts that are defective for messenger RNA processing. Various types of pd-RNA constructs are disclosed, including those defective for endonucleolytic cleavage or polyadenylation at the 3' end of the pd-RNA transcript and/or intron splicing. A pd-RNA construct of the invention may used to suppress a single, specific target gene or multiple target genes. Further, the suppression effect of a pd-RNA construct can be modulated and controlled through the use of an appropriate promoter.
Inventors
- 👤 Nam-Hai Chua
- 👤 Alexander van der Krol
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 18:15
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8241; C12N15/8242; C12N15/8243; C12N15/825; C12N15/8216; C12N15/8218; C12N15/8261; C12N15/8262; C12N15/827
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
99 words
A method for producing a transformed petunia plant cell whose expression of a gene is suppressed, which method comprises: (a) transforming a petunia plant cell, that contains an expressible gene, with a recombinant DNA construct containing a DNA sequence encoding an RNA transcript in which 3' processing signal downstream of the polyadenylation addition site is omitted, wherein the transcription of said recombinant DNA construct results in a processing defective RNA transcript, and wherein the RNA transcript selectively hybridizes to said expressible gene; and (b) selecting a transformed petunia plant tell in which expression of the expressible gene is suppressed.
Claim 2
Independent Claim
99 words
A method for producing a transformed petunia plant cell whose expression of a gene is suppressed, which method comprises: (a) transforming a petunia plant cell, that contains an expressible gene, with a recombinant DNA construct containing a DNA sequence encoding an RNA transcript in which 3' processing signal downstream of the polyadenylation addition site is omitted, wherein the transcription of said recombinant DNA construct results in a processing defective RNA transcript, and wherein the RNA transcript selectively hybridizes to said expressible gene; and (b) selecting a transformed petunia plant tell in which expression of the expressible gene is suppressed.
Claim 3
Dependent Claim
24 words
The method of claim 1 wherein said recombinant DNA construct further comprises a promoter operably linked to the DNA sequence encoding an RNA transcript.
Claim 4
Dependent Claim
22 words
The method of claim 1 wherein said suppressed expression results in altered pigmentation in petunia plants comprising said transformed petunia plant cells.
Claim 5
Dependent Claim
24 words
The method of claim 1 wherein said suppressed expression results in alteration of flower pigmentation in petunia plants comprising said transformed petunia plant cells.
Claim 6
Dependent Claim
24 words
The method of claim 1 wherein said suppressed expression results in inhibition of petal differentiation in petunia plants comprising said transformed petunia plant cells.
Claim 7
Dependent Claim
18 words
A plant regenerated from a transformed petunia plant cell produced in accordance with the method of claim 1.
Claim 8
Dependent Claim
18 words
A cell, tissue, organ, seed or progeny plant obtained from a plant produced in accordance with claim 6.
Claim 9
Independent Claim
134 words
A method for producing a transformed petunia plant whose expression of a gene is suppressed, which method comprises: (a) transforming a petunia plant cell, that contains an expressible gene, with a recombinant DNA construct containing a DNA sequence that encodes an RNA transcript in which 3' processing signal downstream of the polyadenylation addition site is omitted, wherein the transcription of said recombinant DNA construct results in a processing defective RNA transcript, and wherein said RNA transcript selectively hybridizes to said gene; (b) selecting a transformed petunia plant cell; (c) regenerating a transformed petunia plant from said transformed petunia plant cell; and (d) selecting a transformed petunia plant in which expression of said gene is suppressed, wherein said suppressed expression results in inhibition of petal differentiation in petunia plants comprising said transformed petunia plant cells.
Claim 10
Independent Claim
134 words
A method for producing a transformed petunia plant whose expression of a gene is suppressed, which method comprises: (a) transforming a petunia plant cell, that contains an expressible gene, with a recombinant DNA construct containing a DNA sequence that encodes an RNA transcript in which 3' processing signal downstream of the polyadenylation addition site is omitted, wherein the transcription of said recombinant DNA construct results in a processing defective RNA transcript, and wherein said RNA transcript selectively hybridizes to said gene; (b) selecting a transformed petunia plant cell; (c) regenerating a transformed petunia plant from said transformed petunia plant cell; and (d) selecting a transformed petunia plant in which expression of said gene is suppressed, wherein said suppressed expression results in inhibition of petal differentiation in petunia plants comprising said transformed petunia plant cells.
References Cited (Backward Citations)
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