US6573099B2
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US
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Genetic constructs for delaying or repressing the expression of a target gene
Assignee: Commonwealth Scientific and Industrial Research Organisation
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Filing: 1998-06-19
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Granted: 2003-06-03
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Expires: 2018-06-19
Abstract
Abstract The present invention relates generally to synthetic genes for modifying endogenous gene expression in a cell, tissue or organ of a transgenic organism, in particular a transgenic animal or plant. More particularly, the present invention provides novel synthetic genes and genetic constructs which are capable of repressing delaying or otherwise reducing the expression of an endogenous gene or a target gene in an organism when introduced thereto.
Inventors
- 👤 Michael Wayne Graham
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 15:26
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/11; C12N15/113; C12N15/67; C12N15/69; C12N15/82; C12N15/8216; C12N15/8218; C12N15/8241; C12N15/8261; C12N15/8271; C12N15/8279; C12N15/8283; C12N9/00; C12N9/0004; C12N9/0071; C12N9/10; C12N9/1048; C12N9/1051; C12N9/12; C12N9/1241; C12N9/127; C12N9/14; C12N9/48; C12N9/50; C12N9/503; C12N2800/00; C12N2800/10; C12N2800/108; C12N2830/00; C12N2830/001; C12N2830/002; C12N2830/15; C12N2830/38; C12N2830/42; C12N2830/55; C12N2830/60; C12N2840/00; C12N2840/10; C12N2840/102; C12N2840/20
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
119 words
An isolated genetic construct which is capable of delaying, repressing or otherwise reducing the expression of a target gene in an animal cell which is transfected with said genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence, wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, and wherein said at least two copies of said structural gene sequence are placed operably under the control of a single promoter sequence which is operable in said cell, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of said promoter sequence.
Claim 2
Dependent Claim
119 words
An isolated genetic construct which is capable of delaying, repressing or otherwise reducing the expression of a target gene in an animal cell which is transfected with said genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence, wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, and wherein said at least two copies of said structural gene sequence are placed operably under the control of a single promoter sequence which is operable in said cell, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of said promoter sequence.
Claim 3
Dependent Claim
115 words
An isolated genetic construct which is capable of delaying, repressing or otherwise reducing the expression of a target gene in an animal cell which is transfected with said genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence wherein each copy of said structural gene sequence is separately placed under the control of a promoter which is operable in said cell, and wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of an individual promoter sequence.
Claim 4
Dependent Claim
115 words
An isolated genetic construct which is capable of delaying, repressing or otherwise reducing the expression of a target gene in an animal cell which is transfected with said genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence wherein each copy of said structural gene sequence is separately placed under the control of a promoter which is operable in said cell, and wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of an individual promoter sequence.
Claim 5
Dependent Claim
145 words
An isolated genetic construct which is capable of delaying, repressing or otherwise reducing the expression of a target gene in an animal cell which is transfected with said genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence, wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, and wherein said at least two copies of said structural gene sequence are placed operably under the control of a single promoter sequence which is operable in said cell, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of said promoter sequence and wherein at least one other copy of said structural gene sequence is placed operably in the antisense orientation under the control of said promoter sequence.
Claim 6
Dependent Claim
145 words
An isolated genetic construct which is capable of delaying, repressing or otherwise reducing the expression of a target gene in an animal cell which is transfected with said genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence, wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, and wherein said at least two copies of said structural gene sequence are placed operably under the control of a single promoter sequence which is operable in said cell, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of said promoter sequence and wherein at least one other copy of said structural gene sequence is placed operably in the antisense orientation under the control of said promoter sequence.
Claim 7
Dependent Claim
142 words
An isolated genetic construct which is capable of delaying, repressing or otherwise reducing the expression of a target gene in an animal cell which is transfected with said genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence and each copy of said structural gene sequence is separately placed under the control of a promoter which is operable in said cell, and wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of an individual promoter sequence, and wherein at least one other copy of said structural gene sequence is placed operably in the antisense orientation under the control of another individual promoter sequence.
Claim 8
Dependent Claim
142 words
An isolated genetic construct which is capable of delaying, repressing or otherwise reducing the expression of a target gene in an animal cell which is transfected with said genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence and each copy of said structural gene sequence is separately placed under the control of a promoter which is operable in said cell, and wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of an individual promoter sequence, and wherein at least one other copy of said structural gene sequence is placed operably in the antisense orientation under the control of another individual promoter sequence.
Claim 9
Dependent Claim
200 words
An isolated genetic construct which is capable of delaying, repressing or otherwise reducing the expression of a target gene in an animal cell which is transfected with said genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence, wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, and wherein said at least two copies of said structural gene sequence are placed operably under the control of a single promoter sequence which is operable in said cell, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of said promoter sequence, wherein at least one other copy of said structural gene sequence is placed operably in the antisense orientation under the control of said promoter sequence, and wherein said at least one copy of said structural gene sequence that is placed in the sense orientation relative to said promoter and said at least one copy of said structural gene sequence that is placed in the antisense orientation relative to said promoter are spaced from each other by a nucleic acid stuffer fragment.
Claim 10
Dependent Claim
200 words
An isolated genetic construct which is capable of delaying, repressing or otherwise reducing the expression of a target gene in an animal cell which is transfected with said genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence, wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, and wherein said at least two copies of said structural gene sequence are placed operably under the control of a single promoter sequence which is operable in said cell, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of said promoter sequence, wherein at least one other copy of said structural gene sequence is placed operably in the antisense orientation under the control of said promoter sequence, and wherein said at least one copy of said structural gene sequence that is placed in the sense orientation relative to said promoter and said at least one copy of said structural gene sequence that is placed in the antisense orientation relative to said promoter are spaced from each other by a nucleic acid stuffer fragment.
Claim 11
Dependent Claim
20 words
An animal cell comprising the genetic construct of any one of claims 1 - 2 or 3 - 5 .
Claim 12
Independent Claim
114 words
A method of delaying or repressing the expression of a target gene in an animal cell, comprising transfecting said animal cell with a genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence, wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, and wherein said at least two copies of said structural gene sequence are placed operably under the control of a single promoter sequence which is operable in said cell, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of said promoter sequence.
Claim 13
Independent Claim
114 words
A method of delaying or repressing the expression of a target gene in an animal cell, comprising transfecting said animal cell with a genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence, wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, and wherein said at least two copies of said structural gene sequence are placed operably under the control of a single promoter sequence which is operable in said cell, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of said promoter sequence.
Claim 14
Dependent Claim
32 words
The method according to claim 7 , wherein at least one other copy of said structural gene sequence is placed operably in the antisense orientation under the control of said promoter sequence.
Claim 15
Dependent Claim
56 words
The method according to claim 8 , wherein said copy of said structural gene sequence that is placed in the sense orientation relative to said promoter and said copy of said structural gene sequence that is placed in the antisense orientation relative to said promoter are spaced from each other by a nucleic acid stuffer fragment.
Claim 16
Independent Claim
110 words
A method of delaying or repressing the expression of a target gene in an animal cell, comprising expressing in said animal cell a genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence, wherein each copy of said structural gene sequence is separately placed under the control of a promoter which is operable in said cell, and wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of an individual promoter sequence.
Claim 17
Independent Claim
110 words
A method of delaying or repressing the expression of a target gene in an animal cell, comprising expressing in said animal cell a genetic construct, wherein said genetic construct comprises at least two copies of a structural gene sequence, wherein each copy of said structural gene sequence is separately placed under the control of a promoter which is operable in said cell, and wherein said structural gene sequence comprises a nucleotide sequence which is substantially identical to at least a region of said target gene, wherein at least one copy of said structural gene sequence is placed operably in the sense orientation under the control of an individual promoter sequence.
Claim 18
Dependent Claim
30 words
The isolated genetic construct according to any one of claims 1 , 2 or 3 - 5 wherein said region of the target gene is 20 to 30 nucleotides long.
Claim 19
Independent Claim
26 words
The method according to any one of claims 7 - 9 or 10 wherein said region of the target gene is 20 to 30 nucleotides long.
Claim 20
Dependent Claim
26 words
The isolated genetic construct according to any one of claims 1 , 2 or 3 - 5 , comprising two copies of said structural gene sequence.
Claim 21
Dependent Claim
30 words
The isolated genetic construct according to any one of claims 1 , 2 or 3 - 5 wherein said region of the target gene is at least 30 nucleotides long.
Claim 22
Dependent Claim
36 words
The isolated genetic construct according to any one of claims 1 , 2 or 3 - 5 wherein said structural gene sequence comprises a nucleotide sequence that is identical to said region of said target gene.
Claim 23
Independent Claim
26 words
The method according to any one of claims 7 - 9 or 10 , wherein said genetic construct comprises two copies of said structural gene sequence.
Claim 24
Independent Claim
27 words
The method according to any one of claims 7 - 9 or 10 , wherein said region of the target gene is at least 30 nucleotides long.
Claim 25
Independent Claim
33 words
The method according to any one of claims 7 - 9 or 10 , wherein said structural gene sequence comprises a nucleotide sequence that is identical to said region of said target gene.
Claim 26
Dependent Claim
12 words
An animal cell comprising the genetic construct according to claim 11 .
Claim 27
Dependent Claim
12 words
An animal cell comprising the genetic construct according to claim 13 .
Claim 28
Dependent Claim
12 words
An animal cell comprising the genetic construct according to claim 14 .
Claim 29
Dependent Claim
12 words
An animal cell comprising the genetic construct according to claim 15 .
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2006-06-02 | 2006-12-02 | 2007-06-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2010-06-02 | 2010-12-02 | 2011-06-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2014-06-02 | 2014-12-02 | 2015-06-03 | $7700.00 | Window Closed / Lapsed |