Patent US6514767B1 successfully refreshed from Google Patents.
US6514767B1
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US
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Surface enhanced spectroscopy-active composite nanoparticles
Assignee: Becton Dickinson and Co
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Filing: 2000-10-06
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Granted: 2003-02-04
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Expires: 2020-10-06
Abstract
Abstract Metal nanoparticles associated with a spectroscopy-active analyte and surrounded by an encapsulant are useful as sensitive optical tags detectable by surface-enhanced spectroscopy.
Inventors
- 👤 Michael J. Natan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 09:08
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/62; G01N21/63; G01N21/65; G01N21/658; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/54346; G01N33/551; G01N33/553; Y; Y10; Y10T; Y10T436/00; Y10T436/13
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
58 words
A method of tagging a molecule, cell, bead or solid support comprising attaching a particle comprising a surface-enhanced Raman spectroscopy (SERS)-active metal nanoparticle associated with a Raman spectroscopy-active molecule and an encapsulant surrounding said SERS-active metal nanoparticle and said Raman spectroscopy-active molecule to said molecule, cell, bead, or solid support, wherein said particle has a measurable SERS spectrum.
Claim 2
Independent Claim
58 words
A method of tagging a molecule, cell, bead or solid support comprising attaching a particle comprising a surface-enhanced Raman spectroscopy (SERS)-active metal nanoparticle associated with a Raman spectroscopy-active molecule and an encapsulant surrounding said SERS-active metal nanoparticle and said Raman spectroscopy-active molecule to said molecule, cell, bead, or solid support, wherein said particle has a measurable SERS spectrum.
Claim 3
Dependent Claim
26 words
The method of claim 1 , wherein said metal nanoparticle is comprised of a metal selected from the group consisting of Cu, Na, Al, and Cr.
Claim 4
Dependent Claim
13 words
The method of claim 1 wherein said metal nanoparticle is comprised of Au.
Claim 5
Dependent Claim
13 words
The method of claim 1 wherein said metal nanoparticle is comprised of Ag.
Claim 6
Dependent Claim
16 words
The method of claim 1 wherein said metal nanoparticle is less than 200 nm in diameter.
Claim 7
Dependent Claim
15 words
The method of claim 5 wherein said metal nanoparticle has a diameter of 40-100 nm.
Claim 8
Dependent Claim
14 words
The method of claim 1 wherein said encapsulant has a thickness of 1-40 nm.
Claim 9
Dependent Claim
14 words
The method of claim 7 wherein said encapsulant has a thickness of 3-10 nm.
Claim 10
Dependent Claim
50 words
The method of claim 1 , wherein said metal nanoparticle comprises a core overlaid with at least one metal shell, and wherein said core and at least one of said metal shells are comprises of a metal selected from the group consisting of Au, Ag, Cu, Na, Al, and Cr.
Claim 11
Dependent Claim
30 words
The method of claim 1 wherein said metal nanoparticle comprises an alloy of at least two metals selected from the group consisting of Au, Ag, Cu, Na, Al, and Cr.
Claim 12
Dependent Claim
18 words
The method of claim 4 , wherein said spectroscopy-active analyte forms a submonolayer coating on said metal nanoparticle.
Claim 13
Dependent Claim
18 words
The method of claim 1 , wherein said spectroscopy-active analyte forms a monolayer coating on said metal nanoparticle.
Claim 14
Dependent Claim
18 words
The method of claim 1 , wherein said spectroscopy-active analyte forms a multilayer coating on said metal nanoparticle.
Claim 15
Dependent Claim
24 words
The method of claim 11 , wherein said encapsulant is selected from the group consisting of glass, polymers, metals, metal oxides, and metal sulfides.
Claim 16
Dependent Claim
28 words
The method of claim 1 , wherein said encapsulant comprises at least two materials selected from the group consisting of glassed, polymers, metals, metal oxides, and metal sulfides.
Claim 17
Dependent Claim
13 words
The method of claim 1 , wherein said encapsulant is glass oxide (SiOx).
Claim 18
Dependent Claim
12 words
The method of claim 1 , wherein said molecule is a biomolecule.
Claim 19
Dependent Claim
12 words
The method of claim 17 wherein said biomolecule is a nucleic acid.
Claim 20
Dependent Claim
11 words
The method of claim 17 wherein said biomolecule is a protein.
Claim 21
Independent Claim
202 words
A method of encoding the reaction history of a solid support, said method comprising the steps of (a) reacting said solid support with a first reagent under a first reaction condition; (b) attaching to said solid support a first species of particle comprising a surface-enhanced Raman spectroscopy (SERS)-active metal nanoparticle associated with a first Raman spectroscopy-active molecule and an encapsulant surrounding said SERS-active metal nanoparticle and said Raman spectroscopy-active molecule, wherein said first species of particle has a measurable and distinct SERS spectrum, and wherein said first species of particle encodes the first reagent or the first reaction condition; (c) reacting said solid support with a second reagent under a second reaction condition; and (d) attaching to said solid support a second species of particle comprising a SERS-active metal nanoparticle associated with a second Raman spectroscopy-active molecule and an encapsulant surrounding said SERS-active metal nanoparticle and said Raman spectroscopy-active molecule, said second species of particle encoding the second reagent or the second reagent condition, wherein said second species of particle has a measurable and distinct SERS spectrum, and said second species of particle having a different spectrum than said first species of particle; whereby a compound is synthesized on said solid support.
Claim 22
Independent Claim
202 words
A method of encoding the reaction history of a solid support, said method comprising the steps of (a) reacting said solid support with a first reagent under a first reaction condition; (b) attaching to said solid support a first species of particle comprising a surface-enhanced Raman spectroscopy (SERS)-active metal nanoparticle associated with a first Raman spectroscopy-active molecule and an encapsulant surrounding said SERS-active metal nanoparticle and said Raman spectroscopy-active molecule, wherein said first species of particle has a measurable and distinct SERS spectrum, and wherein said first species of particle encodes the first reagent or the first reaction condition; (c) reacting said solid support with a second reagent under a second reaction condition; and (d) attaching to said solid support a second species of particle comprising a SERS-active metal nanoparticle associated with a second Raman spectroscopy-active molecule and an encapsulant surrounding said SERS-active metal nanoparticle and said Raman spectroscopy-active molecule, said second species of particle encoding the second reagent or the second reagent condition, wherein said second species of particle has a measurable and distinct SERS spectrum, and said second species of particle having a different spectrum than said first species of particle; whereby a compound is synthesized on said solid support.
Claim 23
Independent Claim
63 words
A method for conducting an assay comprising: attaching to a molecule a particle comprising a surface-enhanced Raman spectroscopy (SERS)-active metal nanoparticle associated with a Raman spectroscopy-active molecule and an encapsulant surrounding said SERS-active metal nanoparticle and said Raman spectroscopy-active molecule, wherein said particle has a measurable SERS spectrum; and measuring the SERS spectrum of said assay to determine the presence of said molecule.
Claim 24
Independent Claim
63 words
A method for conducting an assay comprising: attaching to a molecule a particle comprising a surface-enhanced Raman spectroscopy (SERS)-active metal nanoparticle associated with a Raman spectroscopy-active molecule and an encapsulant surrounding said SERS-active metal nanoparticle and said Raman spectroscopy-active molecule, wherein said particle has a measurable SERS spectrum; and measuring the SERS spectrum of said assay to determine the presence of said molecule.
Claim 25
Dependent Claim
15 words
The method of claim 1 , further comprising detecting a SERS spectrum of said particle.
Claim 26
Dependent Claim
16 words
The method of claim 20 , further comprising detecting a SERS spectrum of said solid support.
Claim 27
Dependent Claim
77 words
The method of claim 21 , further comprising attaching to a second molecule a second particle comprising a second surface-enhanced Raman spectroscopy (SERS)-active metal nanoparticle associated with a second Raman spectroscopy-active molecule and a second encapsulant surrounding said SERS-active metal nanoparticle and said Raman spectroscopy-active molecule, wherein said second particle has a measurable and distinct SERS spectrum; wherein step (b) further comprises measuring the SERS spectrum of said assay to determine the presence of said second molecule.
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