US5266498A
Expired / Lapsed
US
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Ligand binding assay for an analyte using surface-enhanced scattering (SERS) signal
Assignee: Abbott Laboratories
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Filing: 1991-11-07
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Granted: 1993-11-30
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Expires: 2011-11-07
Abstract
Abstract An assay for an analyte in a test sample that uses a ligand binding reaction between the analyte and a specific binding member or a binding member pair that includes one binding member having a Raman active reporter. The analyte can be assayed by measurement of the Raman scattering signal of the Raman-active reporter. The Raman scattering signal can be either a noon-enhanced Raman scattering signal or a Raman scattering signal that is enhanced by a surface capable of enhancing that Raman scattering signal.
Inventors
- 👤 Peter J. Tarcha
- 👤 Thomas E. Rohr
- 👤 Therese Cotton
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 10:46
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N21/00; G01N21/62; G01N21/63; G01N21/65; G01N21/658; G01N2021/653; Y; Y10; Y10S; Y10S435/00; Y10S435/968; Y10S436/00; Y10S436/80; Y10S436/801; Y10S436/805; Y10T; Y10T436/00; Y10T436/24
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
245 words
A method for determining the presence or amount of an analyte in a test sample, comprising: (a) contacting said test sample with an indicator reagent and a capture reagent to form a test mixture; said indicator reagent comprising a Raman active label directly or indirectly attached to a specific binding member wherein said Raman active label comprises at least one Raman active molecule providing a detectable or measurable Raman scattering signal when illuminated by a radiation capable of inducing a Raman scattering, wherein said specific binding member of said indicator reagent is specific for a substance selected from the group consisting of the analyte, the capture reagent and an ancillary specific binding member which specifically binds to said analyte directly or indirectly through one or more additional ancillary specific binding members; said capture reagent comprising a specific binding member directly or indirectly bound or attached to a surface capable of causing surface-enhanced Raman scattering (SERS), wherein said specific binding member of said capture reagent is specific for a substance selected from the group consisting of the analyte and the indicator reagent; (b) allowing said indicator reagent and said capture reagent to bind to their respective specific binding pair members in said test mixture; (c) inducing a Raman scattering signal by illuminating said surface with a radiation; (d) measuring the intensity of said SERS signal resulting from said illumination of said surface; and (e) determining thereby the presence or amount of analyte in said test sample.
Claim 2
Independent Claim
245 words
A method for determining the presence or amount of an analyte in a test sample, comprising: (a) contacting said test sample with an indicator reagent and a capture reagent to form a test mixture; said indicator reagent comprising a Raman active label directly or indirectly attached to a specific binding member wherein said Raman active label comprises at least one Raman active molecule providing a detectable or measurable Raman scattering signal when illuminated by a radiation capable of inducing a Raman scattering, wherein said specific binding member of said indicator reagent is specific for a substance selected from the group consisting of the analyte, the capture reagent and an ancillary specific binding member which specifically binds to said analyte directly or indirectly through one or more additional ancillary specific binding members; said capture reagent comprising a specific binding member directly or indirectly bound or attached to a surface capable of causing surface-enhanced Raman scattering (SERS), wherein said specific binding member of said capture reagent is specific for a substance selected from the group consisting of the analyte and the indicator reagent; (b) allowing said indicator reagent and said capture reagent to bind to their respective specific binding pair members in said test mixture; (c) inducing a Raman scattering signal by illuminating said surface with a radiation; (d) measuring the intensity of said SERS signal resulting from said illumination of said surface; and (e) determining thereby the presence or amount of analyte in said test sample.
Claim 3
Dependent Claim
19 words
The method according to claim 1, wherein said SERS surface is in the form of a textured silver surface.
Claim 4
Dependent Claim
17 words
The method according to claim 1, wherein the SERS surface is in the form of a colloid.
Claim 5
Dependent Claim
31 words
The method of claim 1 wherein said indicator reagent binds to said analyte and said SERS signal correlates to the presence or quantity of said analyte bound to said capture reagent.
Claim 6
Dependent Claim
25 words
The method of claim 1 further comprising the step of separating the unbound indicator reagent from the bound indicator reagent prior to said measuring step.
Claim 7
Dependent Claim
18 words
The method of claim 5 wherein the bound and the unbound indicator reagent is separated by physical means.
Claim 8
Dependent Claim
13 words
The method of claim 6 wherein said physical means comprises a wash step.
Claim 9
Dependent Claim
24 words
The method of claim 5 wherein the quantity or presence of bound or unbound indicator reagent is determined by measurement of a SERS signal.
Claim 10
Dependent Claim
14 words
The method according to claim 1, wherein the test sample is a biological fluid.
Claim 11
Independent Claim
254 words
A method for performing a ligand-binding assay for determining the presence or amount of an analyte in a test sample, comprising: (a) contacting said test sample with an indicator reagent and a capture reagent to form a test mixture; said indicator reagent comprising a Raman active label directly or indirectly attached to a specific binding member wherein said Raman active label comprises at least one Raman active molecule providing a detectable or measurable Raman scattering signal when illuminated by a radiation capable of inducing a Raman scattering; wherein said specific binding member of said indicator reagent is specific for a substance selected from the group consisting of the analyte, the capture reagent, and an ancillary binding member which specifically binds to said analyte directly or indirectly through one or more additional ancillary specific binding members; said capture reagent comprising a specific binding member directly or indirectly bound or attached to a surface capable of causing a surface-enhanced Raman scattering (SERS) wherein said specific binding member of said capture reagent is specific for a substance selected from the group consisting of the analyte and the indicator reagent; (b) allowing said indicator reagent and said capture reagent to bind to their respective specific binding pair members in said test mixture; (c) illuminating said surface with a radiation so as to induce a SERS signal; (d) measuring the intensity of Raman shift of said SERS signal resulting from the illumination of said surface; and (e) determining thereby the presence or amount of analyte in said test sample.
Claim 12
Independent Claim
254 words
A method for performing a ligand-binding assay for determining the presence or amount of an analyte in a test sample, comprising: (a) contacting said test sample with an indicator reagent and a capture reagent to form a test mixture; said indicator reagent comprising a Raman active label directly or indirectly attached to a specific binding member wherein said Raman active label comprises at least one Raman active molecule providing a detectable or measurable Raman scattering signal when illuminated by a radiation capable of inducing a Raman scattering; wherein said specific binding member of said indicator reagent is specific for a substance selected from the group consisting of the analyte, the capture reagent, and an ancillary binding member which specifically binds to said analyte directly or indirectly through one or more additional ancillary specific binding members; said capture reagent comprising a specific binding member directly or indirectly bound or attached to a surface capable of causing a surface-enhanced Raman scattering (SERS) wherein said specific binding member of said capture reagent is specific for a substance selected from the group consisting of the analyte and the indicator reagent; (b) allowing said indicator reagent and said capture reagent to bind to their respective specific binding pair members in said test mixture; (c) illuminating said surface with a radiation so as to induce a SERS signal; (d) measuring the intensity of Raman shift of said SERS signal resulting from the illumination of said surface; and (e) determining thereby the presence or amount of analyte in said test sample.
Claim 13
Dependent Claim
33 words
The method according to claim 10, wherein said SERS surface allows the differentiation of a bound Raman active label and a free unbound Raman active label during said measuring part of said method.
Claim 14
Dependent Claim
16 words
The method according to claim 10 wherein said SERS surface will support localized photoexcitable surface plasmons.
Claim 15
Dependent Claim
17 words
The method according to claim 10, wherein said SERS surface is in the form of a colloid.
Claim 16
Dependent Claim
19 words
The method according to claim 10, wherein said SERS surface is in the form of a textured silver surface.
Claim 17
Dependent Claim
14 words
The method according to claim 10, wherein the test sample is a biological fluid.
Claim 18
Dependent Claim
26 words
The method of claim 10, wherein said SERS signal or Raman shift correlates to the presence or quantity of said analyte bound to said capture regent.
Claim 19
Dependent Claim
25 words
The method of claim 10 further comprising the step of separating the unbound indicator reagent from the bound indicator reagent prior to said measuring step.
Claim 20
Dependent Claim
18 words
The method of claim 17 wherein the bound and the unbound indicator reagent is separated by physical means.
Claim 21
Dependent Claim
13 words
The method of claim 18, wherein said physical means comprises a wash step.
References Cited (Backward Citations)
GB2156970A
Cited
US4781458A
Cited
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