US6486308B2
Expired / Lapsed
US
⏱️ 0.0 years remaining
Covalently linked oligonucleotide minor groove binder conjugates
Assignee: DRUG ROYALTY TRUST 9
•
Filing: 2000-12-18
•
Granted: 2002-11-26
•
Expires: 2020-12-18
Abstract
Abstract Minor groove binding molecules are covalently bound to oligonucleotides which in their base sequence are complementary to a target sequence of single stranded or double stranded DNA, RNA or hybrids thereof. The covalently bound oligonucleotide minor groove binder conjugates strogly bind to the target sequence of the complementary strand.
Inventors
- 👤 Igor V. Kutyavin
- 👤 Eugeny A. Lukhtanov
- 👤 Howard B. Gamper
- 👤 Rich B. Meyer, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 03:48
CPC Classifications
C; C07; C07H; C07H21/00; C07D; C07D207/00; C07D207/02; C07D207/30; C07D207/34; C07D403/00; C07D403/02; C07D403/12; C07D487/00; C07D487/02; C07D487/04; C07H19/00; C07H19/02; C07H19/04; C07H19/06; C07H19/073; C07H19/16; C07H19/173; C07H19/23; C07H21/04; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
106 words
An oligonucleotide-minor groove binder-reporter group combination comprising an oligonucleotide having a plurality of nucleotide units, a 3′-end and a 5′-end, a minor groove binder moiety attached to at least one of said nucleotide units through a linking group which covalently binds the minor groove binder moiety to the oligonucleotide, and a reporter group; wherein the minor groove binder moiety is a radical of a molecule having a molecular weight of approximately 150 to approximately 2000 Daltons that binds in a non-intercalating manner into the minor groove of double stranded DNA, RNA or hybrids thereof with an association constant greater than approximately 10 3 M −1 .
Claim 2
Dependent Claim
106 words
An oligonucleotide-minor groove binder-reporter group combination comprising an oligonucleotide having a plurality of nucleotide units, a 3′-end and a 5′-end, a minor groove binder moiety attached to at least one of said nucleotide units through a linking group which covalently binds the minor groove binder moiety to the oligonucleotide, and a reporter group; wherein the minor groove binder moiety is a radical of a molecule having a molecular weight of approximately 150 to approximately 2000 Daltons that binds in a non-intercalating manner into the minor groove of double stranded DNA, RNA or hybrids thereof with an association constant greater than approximately 10 3 M −1 .
Claim 3
Dependent Claim
24 words
An oligonucleotide-minor groove binder-reporter group combination of claim 1 wherein the minor groove binding moiety is attached to the 5′ -end of the oligonucleotide.
Claim 4
Dependent Claim
23 words
An oligonucleotide-minor groove binder-reporter group combination of claim 1 wherein the minor groove binding moiety is attached to the 3′-end of the oligonucleotide.
Claim 5
Dependent Claim
35 words
An oligonucleotide-minor groove binder-reporter group combination of claim 1 wherein the minor groove binder moiety is attached to a nucleotide unit which is neither at the 3′ nor at the 5′ end of the oligonucleotide.
Claim 6
Dependent Claim
26 words
An oligonucleotide-minor groove binder-reporter group combination of claim 1 wherein the minor groove binder moiety is attached to the heterocyclic base portion of a nucleotide unit.
Claim 7
Dependent Claim
23 words
An oligonucleotide-minor groove binder-reporter group combination of claim 1 , wherein the reporter group is covalently attached to the minor groove binder moiety.
Claim 8
Dependent Claim
26 words
An oligonucleotide-minor groove binder-reporter group combination of claim 1 , wherein said reporter group is detectable by color, ultraviolet spectrum, or a physical or chemical characteristic.
Claim 9
Dependent Claim
18 words
An oligonucleotide-minor groove binder-reporter group combination of claim 1 , wherein said reporter group is a diazobenzene moiety.
Claim 10
Dependent Claim
47 words
An oligonucleotide-minor groove binder-reporter group combination of claim 1 , wherein said reporter group is attached to said minor groove binder moiety via a—NH(CH 2 ) m COO(CH 2 ) m S(CH 2 ) m —bridge wherein each subscript m is an integer between 1 and 10.
Claim 11
Dependent Claim
48 words
An oligonucleotide-minor groove binder-reporter group combination of claim 1 , wherein said minor groove binder moiety is represented by the formula: wherein the subscript m is an integer of from 2 to 5; and R is a selected from the group consisting of tBOC and CONH 2 .
Claim 12
Dependent Claim
16 words
An oligonucleotide-minor groove binder-reporter group combination of claim 1 , wherein said oligonucleotide is an oligodeoxyribonucleotide.
Claim 13
Dependent Claim
30 words
An oligonucleotide-minor groove binder-reporter group combination of claim 1 , wherein said oligonucleotide is an oligodeoxyribonucleotide having nucleotide units interconnected by phosphodiester linkages, phosphorothioate linkages, methylphosphonate linkages or combinations thereof.
Claim 14
Dependent Claim
36 words
An oligonucleotide-minor groove binder-reporter group combination of claim 1 , wherein said oligonucleotide comprises bases selected from the group consisting of uracil, guanine, adenine, cytosine, thymine, hypoxanthine, 2-aminoadenine, 2-thiouracil, 2-thiothymine, 5-N 4 ethenocytosine, 4-aminopyrazolo[3,4-d]pyrimidine and 6-amino-4-hydroxy-[3,4-d]pyrimidine.
References Cited (Backward Citations)
US4169888A
Cited
US4835263A
Cited
US5519126A
Cited
US4921788A
Cited
WO1991006556A1
Cited
US5466786B1
Cited
US5466786A
Cited
US5237101A
Cited
US5296350A
Cited
WO1992020698A1
Cited
US5419966A
Cited
US5340716A
Cited
US5786138A
Cited
US5395849A
Cited
US5446137A
Cited
US5446137B1
Cited
US5801155A
Cited
US6084102A
Cited
US5955590A
Cited
WO1998049142A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2005-11-25 | 2006-05-27 | 2006-11-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2009-11-25 | 2010-05-27 | 2010-11-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2013-11-25 | 2014-05-27 | 2014-11-26 | $7700.00 | Window Closed / Lapsed |