US5419966A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Solid support for synthesis of 3'-tailed oligonucleotides
Assignee: Nanogen Inc
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Filing: 1993-07-12
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Granted: 1995-05-30
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Expires: 2013-07-12
Abstract
Abstract A solid support for oligonucleotide synthesis has the structure <IMAGE> where CPG represents a controlled pore glass matrix, the wavy line represents a carbon chain covalently linking the NH group with the controlled pore glass matrix, X is 2,2'-dimethoxytrityl or H, and R is alkyl, aryl, arylalkyl, heteroalkyl, or heteroaryl. The dimethoxytrityl group is removed from the solid support by treatment with acid, and the oligonucleotide is built, step-by-step in a conventional synthesizer after attachment of the 3' end of the first oligonucleotide unit to the hydroxyl function connected to the R group.
Inventors
- 👤 Michael W. Reed
- 👤 Rich B. Meyer, Jr.
- 👤 Charles R. Petrie
- 👤 John C. Tabone
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 18:21
CPC Classifications
C; C07; C07D; C07D401/00; C07D401/02; C07D401/06; C07D207/00; C07D207/02; C07D207/04; C07D207/10; C07D207/12; C07H; C07H21/00; Y; Y10; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2991; Y10T428/2993; Y10T428/2996
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
48 words
A solid support for oligonucleotide synthesis having the following structure: wherein CPG represents controlled pore glass, the wavy line represents an alkylamine, which includes the amide nitrogen, covalently attached to the controlled pore glass, X is H or 2,3'-dimethoxytrityl, and R is alkyl, aryl, arylalkyl, heteroalkyl, or heteroaryl.
Claim 2
Dependent Claim
48 words
A solid support for oligonucleotide synthesis having the following structure: wherein CPG represents controlled pore glass, the wavy line represents an alkylamine, which includes the amide nitrogen, covalently attached to the controlled pore glass, X is H or 2,3'-dimethoxytrityl, and R is alkyl, aryl, arylalkyl, heteroalkyl, or heteroaryl.
Claim 3
Dependent Claim
20 words
The solid support of claim 1 wherein R is (CH 2 ) n and n is an integer from 1-10.
Claim 4
Dependent Claim
10 words
The solid support of claim 1 wherein n is 6.
Claim 5
Dependent Claim
10 words
The solid support of claim 1 wherein X is H.
Claim 6
Dependent Claim
10 words
The solid support of claim 1 wherein X is 2,2'-dimethoxytrityl.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-05-29 | 1998-11-28 | 1999-05-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-05-29 | 2002-11-28 | 2003-05-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-05-29 | 2006-11-28 | 2007-05-30 | $7700.00 | Window Closed / Lapsed |