Patent US6149868A successfully refreshed from Google Patents.
US6149868A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Surface enhanced raman scattering from metal nanoparticle-analyte-noble metal substrate sandwiches
Assignee: SICPA Holding SA
•
Filing: 1998-10-28
•
Granted: 2000-11-21
•
Expires: 2018-10-28
Abstract
Abstract A substrate for surface enhanced Raman scattering (SERS) is described in which the analyte is deliberately sandwiched between a colloidal metal nanoparticle and the SERS substrate. In a first embodiment, the analyte is a protein, and the sandwich comprises a protein:Au colloid complex adsorbed to an aggregated Ag sol. Another embodiment concerns a protein:Ag complex, adsorbed to an aggregated Ag sol. In another embodiment, a protein:Au colloid complex is covalently or non-covalently attached to a macroscopic SERS-active substrate. In yet another embodiment, the colloidal nanoparticle is stabilized by a polymeric additive. In yet another embodiment, detection is accomplished at extremely low analyte concentrations. In a final embodiment, control of analyte orientation with respect to the SERS substrate is demonstrated.
Inventors
- 👤 Michael J. Natan
- 👤 Christine Keating
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 15:23
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/62; G01N21/63; G01N21/65; G01N21/658; B; B82; B82Y; B82Y30/00
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
54 words
A nanometer scale structure for surface enhanced Raman scattering (SERS) comprising three proximal components: (a) a SERS-active metal substrate (b) a colloidal metal nanoparticle (c) an analyte sandwiched between the substrate and particle such that a shortest distance between the metal nanoparticle surface and the metal substrate surface is between 1 and 50 nanometers.
Claim 2
Dependent Claim
54 words
A nanometer scale structure for surface enhanced Raman scattering (SERS) comprising three proximal components: (a) a SERS-active metal substrate (b) a colloidal metal nanoparticle (c) an analyte sandwiched between the substrate and particle such that a shortest distance between the metal nanoparticle surface and the metal substrate surface is between 1 and 50 nanometers.
Claim 3
Dependent Claim
26 words
The invention as defined in claim 1 wherein said SERS-active metal substrate is selected from the group consisting of: Au, Ag, Cu, Al, Cr, and Na.
Claim 4
Dependent Claim
33 words
The invention as defined in claim 1 wherein said SERS-active metal substrate comprises an alloy of two or more elements selected from the group consisting of: Au, Ag, Cu, Al, Cr, and Na.
Claim 5
Dependent Claim
34 words
The invention as defined in claim 1 wherein said SERS-active metal substrate comprises an inhomogeneous mixture of two or more elements selected from the group consisting of: Au, Ag, Cu, Al, Cr, and Na.
Claim 6
Dependent Claim
36 words
The invention as defined in claim 1 wherein said SERS-active metal substrate comprises a substance having two or more continuous layers of elements selected from the group consisting of: Au, Ag, Cu, Al, Cr, and Na.
Claim 7
Dependent Claim
50 words
The invention as defined in claim 1 wherein said SERS-active metal substrate comprises a composite material with domains of the elements Au, Ag, Cu, Al, Cr, Na, or an alloy of any two of said elements, such that one dimension of a domain measures between 10 nanometers and 1 millimeter.
Claim 8
Dependent Claim
27 words
The invention as defined in claim 1 wherein said SERS-active metal substrate is selected from the group consisting of aggregated metal nanoparticles, discontinuous films, and continuous films.
Claim 9
Dependent Claim
20 words
The invention as defined in claim 1 wherein said SERS-active metal substrate is coated with a continuous or discontinuous film.
Claim 10
Dependent Claim
33 words
The invention as defined in claim 8 wherein the film is a metal chosen from the 3 rd , 4 th , or 5 th row of the periodic table of the elements.
Claim 11
Dependent Claim
14 words
The invention as defined in claim 8 wherein the film is a self-assembled monolayer.
Claim 12
Dependent Claim
28 words
The invention as defined in claim 8 wherein the film is a polymer, a biopolymer, a mixture of polymers and biopolymers or a mixture of polymers or biopolymers.
Claim 13
Dependent Claim
43 words
The invention as defined in claim 8 wherein the film is a metal oxide, sulfide, nitride, or phosphide, such that the metal is chosen from the 3 rd , 4 th , or 5 th row of the periodic table of the elements.
Claim 14
Dependent Claim
25 words
The invention as defined in claim 1 wherein said metal nanoparticle is selected from the group consisting of: Au, Ag, Cu, Al, Cr, and Na.
Claim 15
Dependent Claim
32 words
The invention as defined in claim 1 wherein said metal nanoparticle comprises an alloy of two or more elements selected from the group consisting of: Au, Ag, Cu, Al, Cr, and Na.
Claim 16
Dependent Claim
33 words
The invention as defined in claim 1 wherein said metal nanoparticle comprises an inhomogeneous mixture of two or more elements selected from the group consisting of: Au, Ag, Cu, Al, Cr, and Na.
Claim 17
Dependent Claim
35 words
The invention as defined in claim 1 wherein said metal nanoparticle comprises a substance having two or more continuous layers of elements selected from the group consisiting of: Au, Ag, Cu, Al, Cr, and Na.
Claim 18
Dependent Claim
32 words
The invention as defined in claim 1 wherein said metal nanoparticle is attached covalently or non-covalently to a carrier consisting of a non-SERS active colloidal particle or a scanned probe microscopy tip.
Claim 19
Dependent Claim
21 words
The invention as defined in claim 1 wherein said metal nanoparticle has at least one dimension between 2 and 200 nanometers.
Claim 20
Dependent Claim
18 words
The invention as defined in claim 1 wherein said metal nanoparticle is spherical, elliptical, cylindrical, cubic, or plate-like.
Claim 21
Dependent Claim
19 words
The invention as defined in claim 1 wherein said metal nanoparticle is coated with a continuous or discontinuous film.
Claim 22
Dependent Claim
33 words
The invention as defined in claim 20 wherein the film is a metal chosen from the 3 rd , 4 th , or 5 th row of the periodic table of the elements.
Claim 23
Dependent Claim
14 words
The invention as defined in claim 20 wherein the film is a self-assembled monolayer.
Claim 24
Dependent Claim
43 words
The invention as defined in claim 20 wherein the film is a metal oxide, sulfide, nitride, or phosphide, such that the metal is chosen from the 3 rd , 4 th , or 5 th row of the periodic table of the elements.
Claim 25
Dependent Claim
21 words
The invention as defined in claim 20 wherein the film comprises a covalently-- or non-covalently bound monolayer of SERS-active metal nanoparticles.
Claim 26
Dependent Claim
22 words
The invention as defined in claim 20 wherein the film is a polymer, a biopolymer, or a mixture of polymers and/or biopolymers.
Claim 27
Dependent Claim
18 words
The invention as defined in claim 20 wherein the film comprises a polypeptide, an oligonucleotide, or a lipid.
Claim 28
Dependent Claim
19 words
The invention as defined in claim 1 wherein said analyte is a molecule, a polymer, or a nanoparticulate substance.
Claim 29
Dependent Claim
20 words
The invention as defined in claim 1 wherein said analyte is bound covalently or non-covalently to the colloidal metal nanoparticle.
Claim 30
Dependent Claim
19 words
The invention as defined in claim 1 wherein said analyte is bound covalently or non-covalently to the SERS-active substrate.
Claim 31
Dependent Claim
16 words
The invention as defined in claim 1 wherein the structure is self-assembled in a single step.
Claim 32
Dependent Claim
16 words
The invention as defined in claim 1 wherein the structure is self-assembled in a stepwise fashion.
References Cited (Backward Citations)
US5266498A
Cited
US5445972A
Cited
US5567628A
Cited
US5552086A
Cited
US5609907A
Cited
US5828450A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2003-11-21 | 2004-05-22 | 2004-11-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2007-11-21 | 2008-05-22 | 2008-11-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2011-11-21 | 2012-05-22 | 2012-11-21 | $7700.00 | Window Closed / Lapsed |