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US6087100A
Expired / Lapsed
US
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Nucleic acid mediated electron transfer
Assignee: California Institute of Technology
•
Filing: 1997-10-08
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Granted: 2000-07-11
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Expires: 2017-10-08
Abstract
Abstract The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.
Inventors
- 👤 Thomas J. Meade
- 👤 Jon Faiz Kayyem
- 👤 Scott E. Fraser
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 09:42
CPC Classifications
C; C07; C07H; C07H23/00; C07H21/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/6818; C12Q1/6825; C12Q1/6827
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
34 words
A single-stranded nucleic acid containing at least one electron donor moiety and at least one electron acceptor moiety, said electron donor moiety and said electron acceptor moiety being covalently attached to said nucleic acid.
Claim 2
Dependent Claim
34 words
A single-stranded nucleic acid containing at least one electron donor moiety and at least one electron acceptor moiety, said electron donor moiety and said electron acceptor moiety being covalently attached to said nucleic acid.
Claim 3
Dependent Claim
26 words
A composition comprising a first single stranded nucleic acid comprising an electrode and a second single stranded nucleic acid comprising a covalently attached electron transfer moiety.
Claim 4
Dependent Claim
26 words
A composition comprising a first single stranded nucleic acid comprising an electrode and a second single stranded nucleic acid comprising a covalently attached electron transfer moiety.
Claim 5
Independent Claim
84 words
A method of detecting a target sequence in a nucleic acid sample comprising: a) applying a first input signal to a hybridization complex comprising said target sequence, which if present, is hybridized to at least a first nucleic acid, wherein said hybridization complex has a covalently attached electron donor moiety and a covalently attached electron acceptor moiety; and b) detecting electron transfer between said electron donor moiety and said electron acceptor moiety as an indication of the presence or absence of said target sequence.
Claim 6
Independent Claim
84 words
A method of detecting a target sequence in a nucleic acid sample comprising: a) applying a first input signal to a hybridization complex comprising said target sequence, which if present, is hybridized to at least a first nucleic acid, wherein said hybridization complex has a covalently attached electron donor moiety and a covalently attached electron acceptor moiety; and b) detecting electron transfer between said electron donor moiety and said electron acceptor moiety as an indication of the presence or absence of said target sequence.
Claim 7
Dependent Claim
22 words
A method according to claim 3 wherein at least one of said electron donor and said electron acceptor moieties is an electrode.
Claim 8
Dependent Claim
24 words
A method according to claim 3 wherein at least one of said electron donor and said electron acceptor moieties is a transition metal complex.
Claim 9
Dependent Claim
24 words
A method according to claim 3 wherein said electron donor moiety is an electrode and said electron acceptor moiety is a transition metal complex.
Claim 10
Dependent Claim
25 words
A method according to claim 3 wherein said first nucleic acid comprises a covalently attached electron donor moiety and a covalently attached electron acceptor moiety.
Claim 11
Dependent Claim
29 words
A method according to claim 3 wherein said first nucleic acid comprises a covalently attached electron donor moiety and said target sequence comprises a covalently attached electron acceptor moiety.
Claim 12
Dependent Claim
25 words
A method according to claim 3 wherein at least one of said electron donor and said electron acceptor moieties is attached to a terminal base.
Claim 13
Dependent Claim
14 words
A method according to claim 3 wherein said nucleic acid comprises a ribose-phosphate backbone.
Claim 14
Dependent Claim
28 words
A method according to claim 10 wherein at least one of said electron donor and said electron acceptor moieties is attached to a ribose of said ribose-phosphate backbone.
Claim 15
Dependent Claim
28 words
A method according to claim 10 wherein at least one of said electron donor and said electron acceptor moieties is attached to a phosphate of said ribose-phosphate backbone.
Claim 16
Dependent Claim
15 words
A method according to claim 3 wherein said nucleic acid is a nucleic acid analog.
Claim 17
Dependent Claim
15 words
A method according to claim 13 wherein said nucleic acid analog is peptide nucleic acid.
Claim 18
Dependent Claim
19 words
A method according to claim 14 wherein said transition metal complex comprises ruthenium, rhenium, osmium, platinum, copper or iron.
Claim 19
Dependent Claim
26 words
A method according to claim 3 wherein at least one of said electron donor and said electron acceptor moieties is an organic electron donor or acceptor.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2003-07-11 | 2004-01-10 | 2004-07-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2007-07-11 | 2008-01-10 | 2008-07-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2011-07-11 | 2012-01-10 | 2012-07-11 | $7700.00 | Window Closed / Lapsed |