US6069006A
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US
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Connective tissue growth factor (CTGF) regulatory nucleic acid sequences
Assignee: University of South Florida St. Petersburg
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Filing: 1998-04-02
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Granted: 2000-05-30
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Expires: 2018-04-02
Abstract
Abstract The present invention provides a novel polypeptide, Connective Tissue Growth Factor (CTGF), polynucleotides encoding CTGF, and including 5' and 3' untranslated nucleotides, antibodies reactive with CTGF and means for producing CTGF. Also provided are diagnostic and therapeutic methods for using CTGF, as well as an assay for identifying compositions which affect the expression of CTGF polynucleotide. The invention provides a novel TGF- beta responsive element upstream of the polynucleotide encoding CTGF structural gene.
Inventors
- 👤 Gary R. Grotendorst
- 👤 Douglass M. Bradham, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 11:20
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; A; A61; A61K; A61K38/00; A61K38/16; A61K38/17; A61K38/18; A61K38/1841; A61K38/39; A61P; A61P11/00; A61P13/00; A61P13/12; A61P17/00; A61P17/02; A61P19/00; A61P19/04; A61P27/00; A61P27/02; A61P27/06; A61P35/00; A61P43/00; A61P9/00; A61P9/10; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0652; C12N5/0655
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
42 words
An isolated polynucleotide comprising 5' untranslated regulatory nucleotide sequences isolated from upstream of a Connective Tissue Growth Factor (CTGF) gene, wherein said regulatory nucleotide sequences comprise a sequence as set forth in SEQ ID NO: 1 from about nucleotides 1 to 897.
Claim 2
Dependent Claim
42 words
An isolated polynucleotide comprising 5' untranslated regulatory nucleotide sequences isolated from upstream of a Connective Tissue Growth Factor (CTGF) gene, wherein said regulatory nucleotide sequences comprise a sequence as set forth in SEQ ID NO: 1 from about nucleotides 1 to 897.
Claim 3
Dependent Claim
18 words
The polynucleotide of claim 1, wherein said untranslated regulatory nucleotide sequences comprises a transcriptional and translational initiation region.
Claim 4
Dependent Claim
20 words
An isolated polynucleotide consisting of the sequence 5'-GTGTCAAGGGGTC-3' (SEQ ID NO: 8), wherein said sequence is a TGF-beta responsive element.
Claim 5
Dependent Claim
20 words
An isolated polynucleotide consisting of the sequence 5'-GTGTCAAGGGGTC-3' (SEQ ID NO: 8), wherein said sequence is a TGF-beta responsive element.
Claim 6
Dependent Claim
18 words
An isolated polynucleotide comprising a TGF-β regulatory element, said element comprising nucleotides 1-698 of SEQ ID NO: 1.
Claim 7
Dependent Claim
18 words
An isolated polynucleotide comprising a TGF-β regulatory element, said element comprising nucleotides 1-698 of SEQ ID NO: 1.
Claim 8
Dependent Claim
14 words
An isolated TGF-β regulatory element, consisting of nucleotides 664-698 of SEQ ID NO: 1.
Claim 9
Dependent Claim
14 words
An isolated TGF-β regulatory element, consisting of nucleotides 664-698 of SEQ ID NO: 1.
Claim 10
Dependent Claim
76 words
An isolated nucleic acid construct comprising a non-coding regulatory sequence isolated upstream from a Connective Tissue Growth Factor (CTGF) gene, wherein said non-coding regulatory sequence is operably associated with a nucleic acid sequence which expresses a protein of interest or antisense RNA, wherein said nucleic acid sequence is heterologous to said non-coding sequence, and wherein the non-coding regulatory sequence comprises a sequence as set forth in SEQ ID NO: 1 from about nucleotides 1 to 897.
Claim 11
Dependent Claim
76 words
An isolated nucleic acid construct comprising a non-coding regulatory sequence isolated upstream from a Connective Tissue Growth Factor (CTGF) gene, wherein said non-coding regulatory sequence is operably associated with a nucleic acid sequence which expresses a protein of interest or antisense RNA, wherein said nucleic acid sequence is heterologous to said non-coding sequence, and wherein the non-coding regulatory sequence comprises a sequence as set forth in SEQ ID NO: 1 from about nucleotides 1 to 897.
Claim 12
Dependent Claim
16 words
The nucleic acid construct of claim 6, wherein said construct is contained in a host cell.
References Cited (Backward Citations)
US5356630A
Cited
US5149691A
Cited
WO1993000430A1
Cited
US5408040A
Cited
US5585270A
Cited
US5399361A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2003-05-30 | 2003-11-29 | 2004-05-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2007-05-30 | 2007-11-29 | 2008-05-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2011-05-30 | 2011-11-29 | 2012-05-30 | $7700.00 | Window Closed / Lapsed |