US6018030A
Expired / Lapsed
US
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Peptides comprising repetitive units of amino acids and DNA sequences encoding the same
Assignee: Syntro Corp
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Filing: 1995-06-07
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Granted: 2000-01-25
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Expires: 2015-06-07
Abstract
Abstract Polypeptides comprising repetitive units of amino acids, as well as synthetic genes encoding the subject polypeptides are provided. The subject polypeptides are characterized by comprising repetitive units of amino acids, where the repetitive units are present in naturally occurring proteins, particularly naturally occurring structural proteins. The subject polypeptides find use in a variety of applications, such as structural components of prosthetic devices, synthetic fibers, and the like.
Inventors
- 👤 Franco A. Ferrari
- 👤 Charles Richardson
- 👤 James Chambers
- 👤 Stuart Causey
- 👤 Thomas J. Pollock
- 👤 Joseph Cappello
- 👤 John W. Crissman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 23:00
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/43504; C07K14/43563; C07K14/43586; C07K14/78; C12; C12N; C12N1/00; C12N1/06; C12N15/00; C12N15/09; C12N15/11; C12N15/62; C12N15/63; C12N15/66; C12N15/70; C12N15/74; C12N15/75; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/33; C07K2319/61
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
78 words
A recombinantly produced protein of from 30 kDal to 250 kDal comprising at least 50 number percent of amino acids of at least one naturally occurring repeating unit of a naturally occurring structural protein, wherein said repeating units are from 4 to 20 amino acids in length and wherein each of said repeating units which comprise said at least 50 number percent of the amino acids of said protein are tandemly repeated at least once in the protein.
Claim 2
Dependent Claim
78 words
A recombinantly produced protein of from 30 kDal to 250 kDal comprising at least 50 number percent of amino acids of at least one naturally occurring repeating unit of a naturally occurring structural protein, wherein said repeating units are from 4 to 20 amino acids in length and wherein each of said repeating units which comprise said at least 50 number percent of the amino acids of said protein are tandemly repeated at least once in the protein.
Claim 3
Dependent Claim
20 words
The recombinantly produced protein according to claim 1, wherein said repeating units are of from 4 to 15 amino acids.
Claim 4
Dependent Claim
20 words
The recombinantly produced protein according to claim 1, wherein said repeating units are of from 4 to 9 amino acids.
Claim 5
Dependent Claim
10 words
A formed object comprising the protein according to claim 1.
Claim 6
Dependent Claim
27 words
The recombinantly produced protein according to claim 1, wherein said repeating units are present in at least 2 blocks of at least 2 repeating units per block.
Claim 7
Dependent Claim
32 words
The recombinantly produced protein according to claim 5, wherein said protein comprises an intervening group of from 1 to 50 amino acids between at least 2 of said blocks of repeating units.
Claim 8
Dependent Claim
10 words
A DNA sequence encoding the protein according to claim 1.
Claim 9
Dependent Claim
85 words
A recombinantly produced protein of from 30 kDal to 250 kDal comprising at least 50 number percent of amino acids of at least one naturally occurring repeating unit of a naturally occurring structural protein selected from the group consisting of elastin, silk, and keratin and of from 4 to 20 amino acids in length and wherein each of said repeating units which comprise said at least 50 number percent of the amino acids of said protein are tandemly repeated at least once in the protein.
Claim 10
Dependent Claim
85 words
A recombinantly produced protein of from 30 kDal to 250 kDal comprising at least 50 number percent of amino acids of at least one naturally occurring repeating unit of a naturally occurring structural protein selected from the group consisting of elastin, silk, and keratin and of from 4 to 20 amino acids in length and wherein each of said repeating units which comprise said at least 50 number percent of the amino acids of said protein are tandemly repeated at least once in the protein.
Claim 11
Dependent Claim
31 words
The recombinantly produced protein according to claim 8, wherein said at least one repeating unit is selected from the group consisting of SGAGAG (SEQ ID NO:21) and GAGAGS (SEQ ID NC:41).
Claim 12
Dependent Claim
35 words
The recombinantly produced protein according to claim 8, wherein said at least one repeating unit is selected from the group consisting of GVGVP (SEQ ID NO:23), VPGVG (SEQ ID NO:24) and APGVGV (SEQ ID NO:25).
Claim 13
Dependent Claim
49 words
The recombinantly produced protein according to claim 8, wherein said at least one repeating unit is K-L-(1)-L-A-E-A (SEQ ID NO:105), wherein 1 is a basic or acidic amino acid, particularly K or E and the repeating units alternate as to whether 1 is a basic or acidic amino acid.
Claim 14
Dependent Claim
10 words
A formed object comprising the protein according to claim 8.
Claim 15
Dependent Claim
27 words
The recombinantly produced protein according to claim 8, wherein said repeating units are present in at least 2 blocks of at least 2 repeating units per block.
Claim 16
Dependent Claim
32 words
The recombinantly produced protein according to claim 13, wherein said protein comprises an intervening group of from 1 to 50 amino acids between at least 2 of said blocks of repeating units.
Claim 17
Dependent Claim
10 words
A DNA sequence encoding the protein according to claim 8.
Claim 18
Dependent Claim
57 words
A recombinantly produced protein of from 30kDal to 250kDal comprising at least 50 number percent of amino acids of at least one naturally occurring repeating unit of the amino acid sequence Gly-α-β, wherein α and β are any amino acid and are selected so that the encoded protein comprises from about 10 to 45 number percent proline.
Claim 19
Dependent Claim
57 words
A recombinantly produced protein of from 30kDal to 250kDal comprising at least 50 number percent of amino acids of at least one naturally occurring repeating unit of the amino acid sequence Gly-α-β, wherein α and β are any amino acid and are selected so that the encoded protein comprises from about 10 to 45 number percent proline.
Claim 20
Dependent Claim
10 words
A formed object comprising the protein according to claim 16.
Claim 21
Dependent Claim
10 words
A DNA sequence encoding the protein according to claim 16.
Claim 22
Dependent Claim
16 words
A cell comprising the DNA sequence according to any one of claims 7, 15 or 18.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2003-01-24 | 2003-07-26 | 2004-01-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2007-01-24 | 2007-07-26 | 2008-01-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2011-01-24 | 2011-07-26 | 2012-01-25 | $7700.00 | Window Closed / Lapsed |