Patent US3093827A successfully refreshed from Google Patents.
Patent US5952172A successfully refreshed from Google Patents.
US5952172A
Expired / Lapsed
US
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Nucleic acid mediated electron transfer
Assignee: California Institute of Technology
•
Filing: 1997-06-12
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Granted: 1999-09-14
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Expires: 2017-06-12
Abstract
Abstract The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions. The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.
Inventors
- 👤 Thomas J. Meade
- 👤 Jon Faiz Kayyem
- 👤 Scott E. Fraser
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 18:22
CPC Classifications
C; C07; C07H; C07H21/00; C07H23/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/6818; C12Q1/6825; Y; Y02; Y02A; Y02A50/00; Y02A50/30
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
82 words
A method for detecting the presence of a target sequence in a nucleic acid sample, comprising: a) applying an AC input signal to a hybridization complex, wherein said hybridization complex comprises i) a first single stranded nucleic acid containing at least one covalently attached electron transfer moiety and an electrode; and ii) a target single stranded nucleic acid; b) detecting the presence of said target sequence via an output signal characteristic of electron transfer between said electrode and said electron transfer moiety.
Claim 2
Independent Claim
82 words
A method for detecting the presence of a target sequence in a nucleic acid sample, comprising: a) applying an AC input signal to a hybridization complex, wherein said hybridization complex comprises i) a first single stranded nucleic acid containing at least one covalently attached electron transfer moiety and an electrode; and ii) a target single stranded nucleic acid; b) detecting the presence of said target sequence via an output signal characteristic of electron transfer between said electrode and said electron transfer moiety.
Claim 3
Independent Claim
83 words
A method for detecting the presence of a target sequence in a nucleic acid sample, comprising: a) applying an AC input signal to a hybridization complex, wherein said hybridization complex comprises i) a first single stranded nucleic acid containing at least one covalently attached electron transfer moiety; and ii) a second single stranded nucleic acid containing an electrode; and b) detecting the presence of said target sequence via an output signal characteristic of electron transfer between said electrode and said electron transfer moiety.
Claim 4
Independent Claim
83 words
A method for detecting the presence of a target sequence in a nucleic acid sample, comprising: a) applying an AC input signal to a hybridization complex, wherein said hybridization complex comprises i) a first single stranded nucleic acid containing at least one covalently attached electron transfer moiety; and ii) a second single stranded nucleic acid containing an electrode; and b) detecting the presence of said target sequence via an output signal characteristic of electron transfer between said electrode and said electron transfer moiety.
Claim 5
Dependent Claim
22 words
A method according to claim 1 or 2 wherein at least one of said electron transfer moieties is a transition metal complex.
Claim 6
Dependent Claim
23 words
A method according to claim 1 or 2 wherein at least one of said electron transfer moieties is an organic electron transfer moiety.
Claim 7
Dependent Claim
20 words
A method according to claim 1 or 2 wherein the AC input signal is applied at a plurality of frequencies.
Claim 8
Dependent Claim
15 words
A method according to claim 1 or 2 wherein said output signal comprises a current.
Claim 9
Dependent Claim
21 words
A method according to claim 1 or 2 wherein said output signal is time delayed as compared to said input signal.
Claim 10
Dependent Claim
31 words
A method according to claim 1 or 2 wherein said nucleic acid comprises a ribose-phosphate backbone and said electron transfer moiety is covalently attached to a ribose of the ribose-phosphate backbone.
Claim 11
Dependent Claim
31 words
A method according to claim 1 or 2 wherein said nucleic acid comprises a ribose-phosphate backbone and said electron transfer moiety is covalently attached to a phosphate of the ribose-phosphate backbone.
Claim 12
Dependent Claim
20 words
A method according to claim 1 or 2 wherein said electron transfer moiety is covalently attached to a terminal base.
Claim 13
Independent Claim
102 words
A method for detecting the presence of a target sequence in a nucleic acid sample, comprising: a) applying an AC input signal at a first frequency to a hybridization complex, wherein said hybridization complex comprises i) a first single stranded nucleic acid containing at least one covalently attached electron transfer moiety and an electrode; and ii) a target single stranded nucleic acid; b) applying an AC input signal at at least a second frequency to said hybridization complex; c) detecting the presence of said target sequence via an output signal characteristic of electron transfer between said electrode and said electron transfer moiety.
Claim 14
Independent Claim
102 words
A method for detecting the presence of a target sequence in a nucleic acid sample, comprising: a) applying an AC input signal at a first frequency to a hybridization complex, wherein said hybridization complex comprises i) a first single stranded nucleic acid containing at least one covalently attached electron transfer moiety and an electrode; and ii) a target single stranded nucleic acid; b) applying an AC input signal at at least a second frequency to said hybridization complex; c) detecting the presence of said target sequence via an output signal characteristic of electron transfer between said electrode and said electron transfer moiety.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-09-13 | 2003-03-15 | 2003-09-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-09-13 | 2007-03-15 | 2007-09-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-09-13 | 2011-03-15 | 2011-09-14 | $7700.00 | Window Closed / Lapsed |