Patent US5948666A successfully refreshed from Google Patents.
US5948666A
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Isolation and identification of polymerases
Assignee: BASF Enzymes LLC
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Filing: 1997-08-06
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Granted: 1999-09-07
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Expires: 2017-08-06
Abstract
Abstract The invention provides purified thermostable enzymes derived from various extremophilic prokaryotic organisms. The enzymes have polymerase activity and can be used to catalyze DNA synthesis by addition of deoxynucleotides to the 3' end of a polynucleotide chain, using a complementary polynucleotide strands as a template.
Inventors
- 👤 Walter Callen
- 👤 Eric J. Mathur
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 13:09
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/10; C12N9/12; C12N9/1241; C12N9/1252
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
85 words
An isolated polynucleotide selected from the group consisting of: a) SEQ IDNO:1, 3, 5, 7, 9, 11; b) SEQ ID NO: 1, 3, 5, 7, 9, 11 wherein T can also be U; c) nucleic acid sequences complementary to a) and b); and d) fragments of a), b), or c) that are at least 15 bases in length and that will hybridize to DNA which encodes the amino acid sequences of SEQ ID NO:2, 4, 6, 8, or 12 under moderate to highly stringent conditions.
Claim 2
Dependent Claim
85 words
An isolated polynucleotide selected from the group consisting of: a) SEQ IDNO:1, 3, 5, 7, 9, 11; b) SEQ ID NO: 1, 3, 5, 7, 9, 11 wherein T can also be U; c) nucleic acid sequences complementary to a) and b); and d) fragments of a), b), or c) that are at least 15 bases in length and that will hybridize to DNA which encodes the amino acid sequences of SEQ ID NO:2, 4, 6, 8, or 12 under moderate to highly stringent conditions.
Claim 3
Dependent Claim
27 words
An isolated polynucleotide sequence encoding a polymerase having an amino acid sequence selected from the group consisting of SEO ID NO:2, 4, 6, 8, 10 and 12.
Claim 4
Dependent Claim
27 words
An isolated polynucleotide sequence encoding a polymerase having an amino acid sequence selected from the group consisting of SEO ID NO:2, 4, 6, 8, 10 and 12.
Claim 5
Dependent Claim
13 words
The polynucleotide of claim 2, wherein the polynucleotide is isolated from a prokaryote.
Claim 6
Dependent Claim
9 words
An expression vector including the polynucleotide of claim 2.
Claim 7
Dependent Claim
11 words
The vector of claim 4, wherein the vector is a plasmid.
Claim 8
Dependent Claim
13 words
The vector of claim 4, wherein the vector is derived from a virus.
Claim 9
Dependent Claim
11 words
A host cell stably transformed with the vector of claim 4.
Claim 10
Dependent Claim
11 words
The host cell of claim 7, wherein the cell is prokaryotic.
Claim 11
Dependent Claim
11 words
The host cell of claim 7, wherein the cell is eukaryotic.
Claim 12
Dependent Claim
35 words
A method for producing a polypeptide comprising: a) culturing the host cells of claim 7; b) expressing from the host cell of claim 7 a polypeptide encoded by said DNA; and c) isolating the polypeptide.
Claim 13
Dependent Claim
33 words
A process for producing a cell comprising transforming or transfecting the cell with the vector of claim 4 such that the cell expresses the polypeptide encoded by the DNA contained in the vector.
Claim 14
Dependent Claim
100 words
An isolated polynucleotide selected from the group consisting of: a) a polynucleotide encoding an enzyme comprising an amino acid sequence selected from the group of amino acid sequences set forth in SEQ ID NOS:2, 4, 6, 8, and 12; b) a polynucleotide which is complementary to the polynucleotide of a); c) a polynucleotide comprising at least 15 bases of the polynucleotide of a) or b) and hybridizes to DNA which encodes an amino acid sequence selected from the group of amino acid sequences set forth in SEQ ID NOS:2, 4, 6, 8, and 12 under moderate to highly stringent conditions.
Claim 15
Dependent Claim
100 words
An isolated polynucleotide selected from the group consisting of: a) a polynucleotide encoding an enzyme comprising an amino acid sequence selected from the group of amino acid sequences set forth in SEQ ID NOS:2, 4, 6, 8, and 12; b) a polynucleotide which is complementary to the polynucleotide of a); c) a polynucleotide comprising at least 15 bases of the polynucleotide of a) or b) and hybridizes to DNA which encodes an amino acid sequence selected from the group of amino acid sequences set forth in SEQ ID NOS:2, 4, 6, 8, and 12 under moderate to highly stringent conditions.
Claim 16
Dependent Claim
21 words
An isolated polynucleotide encoding an enzyme comprising an amino acid sequence set forth in SEQ ID NO:10 and polynucleotides complementary thereto.
Claim 17
Dependent Claim
21 words
An isolated polynucleotide encoding an enzyme comprising an amino acid sequence set forth in SEQ ID NO:10 and polynucleotides complementary thereto.
References Cited (Backward Citations)
USH1531H
Cited
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