USH1531H
Expired / Lapsed
US
⏱️ 0.0 years remaining
Thermophilic DNA polymerase
Assignee: Life Technologies Corp
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Filing: 1994-04-18
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Granted: 1996-05-07
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Expires: 2014-04-18
Abstract
Abstract The invention relates to a substantially pure thermostable DNA polymerase. Preferably, the DNA polymerase has a molecular weight of about 95 kilodaltons and is more thermostable than Taq DNA polymerase. The present invention also relates to cloning and expression of the DNA polymerase in E. coli, to DNA molecules containing the cloned gene, and to host cells which express said genes.
Inventors
- 👤 Ilse I. Blumentals
- 👤 Roger S. Lasken
- 👤 Brian J. Schmidt
- 👤 Mary C. Longo
- 👤 A. John Hughes, Jr.
- 👤 Deb K. Chatterjee
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 13:53
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/10; C12N9/12; C12N9/1241; C12N9/1252
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
16 words
A substantially pure DNA polymerase comprising the amino acid sequence set forth in SEQ ID NO:2.
Claim 2
Dependent Claim
16 words
A substantially pure DNA polymerase comprising the amino acid sequence set forth in SEQ ID NO:2.
Claim 3
Dependent Claim
37 words
A substantially pure DNA polymerase comprising the amino acid sequence set forth in SEQ ID NO:2 except wherein asparagine at position 141 has been replaced by alanine and glutamine at position 143 has been replaced by alanine.
Claim 4
Dependent Claim
37 words
A substantially pure DNA polymerase comprising the amino acid sequence set forth in SEQ ID NO:2 except wherein asparagine at position 141 has been replaced by alanine and glutamine at position 143 has been replaced by alanine.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-05-07 | 1999-11-06 | 2000-05-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-05-07 | 2003-11-06 | 2004-05-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-05-07 | 2007-11-06 | 2008-05-07 | $7700.00 | Window Closed / Lapsed |