Patent US4097387A successfully refreshed from Google Patents.
US4889818A
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Purified thermostable enzyme
Assignee: Roche Molecular Systems Inc
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Filing: 1987-06-17
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Granted: 1989-12-26
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Expires: 2007-06-17
Abstract
Abstract A purified thermostable enzyme is obtained that has unique characteristics. Preferably the enzyme is isolated from the Thermus aquaticus species and has a molecular weight of about 86,000-90,000 daltons. The thermostable enzyme may be native or recombinant and may be used in a temperature-cycling chain reaction wherein at least one nucleic acid sequence is amplified in quantity from an existing sequence with the aid of selected primers and nucleotide triphosphates. The enzyme is preferably stored in a buffer of non-ionic detergents that lends stability to the enzyme.
Inventors
- 👤 David H. Gelfand
- 👤 Susanne Stoffel
- 👤 Frances C. Lawyer
- 👤 Randall K. Saiki
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 11:40
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/26; C12P19/28; C12P19/30; C12P19/34; C12N; C12N9/00; C12N9/10; C12N9/12; C12N9/1241; C12N9/1252; C12N9/1276
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
48 words
Purified thermostable Thermus aquaticus DNA polymerase that migrates on a denaturing polyacrylamide gel faster than phosphorylase B and more slowly than does bovine serum albumin and has an estimated molecular weight of 86,000-90,000 daltons when compared with a phosphorylase B standard assigned a molecular weight of 92,500 daltons
Claim 2
Dependent Claim
48 words
Purified thermostable Thermus aquaticus DNA polymerase that migrates on a denaturing polyacrylamide gel faster than phosphorylase B and more slowly than does bovine serum albumin and has an estimated molecular weight of 86,000-90,000 daltons when compared with a phosphorylase B standard assigned a molecular weight of 92,500 daltons
Claim 3
Dependent Claim
11 words
The polymerase of claim 1 that is isolated from Thermus acquaticus.
Claim 4
Dependent Claim
26 words
The polymerase of claim 1 that is isolated from a recombinant organism transformed with a vector that codes for the expression of Thermis aquaticus DNA polymerase.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-12-25 | 1993-06-26 | 1993-12-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-12-25 | 1997-06-26 | 1997-12-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-12-25 | 2001-06-26 | 2001-12-26 | $7700.00 | Window Closed / Lapsed |