US5876989A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Transfer of molecules into the cytosol of cells
Assignee: Photocure ASA
•
Filing: 1995-09-04
•
Granted: 1999-03-02
•
Expires: 2015-09-04
Abstract
Abstract A method for releasing molecules into the cytosol of cells without killing the majority of the cells by allowing the molecules to be taken up in endosomes, lysosomes or other cell compartments and use light activation of photosensitizing compounds to rupture the membranes of the endosomes, lysosomes or other cell compartments, is described.
Inventors
- 👤 Kristian Berg
- 👤 Kirsten Sandvig
- 👤 Johan Moan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 17:28
CPC Classifications
A; A61; A61K; A61K41/00; A61K41/0057; A61K41/0071; A61K47/00; A61K47/50; A61P; A61P35/00
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
84 words
A method for introducing a molecule of interest into the cytosol of a living cell, comprising: a) delivering a photosensitizing compound, the molecule of interest, and a carrier molecule to the cell, wherein each are taken up into an intracellular compartment of the cell; b) irradiating the cell with light of a suitable wavelength to activate the photosensitizing compound so that the membrane surrounding the intracellular compartment is disrupted, releasing the molecule of interest into the cytosol of the cell without killing the cell.
Claim 2
Independent Claim
84 words
A method for introducing a molecule of interest into the cytosol of a living cell, comprising: a) delivering a photosensitizing compound, the molecule of interest, and a carrier molecule to the cell, wherein each are taken up into an intracellular compartment of the cell; b) irradiating the cell with light of a suitable wavelength to activate the photosensitizing compound so that the membrane surrounding the intracellular compartment is disrupted, releasing the molecule of interest into the cytosol of the cell without killing the cell.
Claim 3
Dependent Claim
36 words
The method according to claim 1 wherein the molecule of interest is DNA, an oligonucleotide, mRNA, antisense DNA, a sugar, a protein, a peptide, a membrane impermeable molecule, or a covalently or noncovalently bonded combination thereof.
Claim 4
Dependent Claim
19 words
The method according to claim 1, wherein the molecule of interest is gelonin, saporin, agrostin, or a combination thereof.
Claim 5
Dependent Claim
36 words
The method according to claim 1, wherein the photosensitizing compound is a porphyrin, a phthalocyanine, a purpurin, a chlorin, a benzoporphyrin, a napthalocyanine, a cationic dye, a tetracycline, or a lysosomotropic weak base or derivative thereof.
Claim 6
Dependent Claim
51 words
The method according to claim 4, wherein the photosensitizing compound is tetraphenyl porphine with 2 sulfonate groups on adjacent phenyl groups (TPPS 2a ), meso-tetraphenyl porphine with 4 sulfonate groups (TPPS 4 ), or aluminum phthalocyanine with 2 sulfonate groups on adjacent phenyl rings (AlPcS 2 ) or a combination thereof.
Claim 7
Dependent Claim
34 words
The method according to claim 1, further comprising providing a vector molecule which facilitates the uptake of either the photosensitizing compound or the molecule of interest which is to be released into the cytosol.
Claim 8
Dependent Claim
48 words
The method according to claim 1, wherein the method is applied to a plurality of cells and wherein the step of irradiating includes selecting a light dose and wavelength and a photosensitizing compound so that after the step of irradiation, a portion of the living cells are killed.
References Cited (Backward Citations)
US4649151A
Cited
US5066274A
Cited
US5095030A
Cited
NO891491L
Cited
GB2209468A
Cited
WO1990000393A1
Cited
US5059619A
Cited
US5179120A
Cited
NO934837L
Cited
NO924151L
Cited
WO1993014142A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-03-01 | 2002-08-31 | 2003-03-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-03-01 | 2006-08-31 | 2007-03-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-03-01 | 2010-08-31 | 2011-03-02 | $7700.00 | Window Closed / Lapsed |