US5871928A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Methods for nucleic acid analysis
Assignee: Affymetrix Inc
•
Filing: 1997-06-11
•
Granted: 1999-02-16
•
Expires: 2017-06-11
Abstract
Abstract The present invention provides methods and apparatus for sequencing, fingerprinting and mapping biological macromolecules, typically biological polymers. The methods make use of a plurality of sequence specific recognition reagents which can also be used for classification of biological samples, and to characterize their sources.
Inventors
- 👤 Stephen P. A. Fodor
- 👤 Dennis W. Solas
- 👤 William J. Dower
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 15:28
CPC Classifications
G; G11; G11C; G11C13/00; G11C13/0002; G11C13/0009; G11C13/0014; G11C13/0019; B; B01; B01J; B01J19/00; B01J19/0046; B82; B82Y; B82Y10/00; B82Y30/00; C; C07; C07H; C07H19/00; C07H19/02; C07H19/04; C07H19/06; C07H19/10; C07H21/00; C07K; C07K1/00; C07K1/04; C07K1/042; C07K1/045; C07K17/00; C07K17/02; C07K17/06; C07K17/14; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; G03; G03F; G03F7/00; G03F7/26; G03F7/265; G03F7/38; G11C13/02; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00279; B01J2219/00306; B01J2219/00313; B01J2219/00315; B01J2219/00351; B01J2219/00427; B01J2219/00432; B01J2219/00434; B01J2219/00436; B01J2219/00457; B01J2219/00459; B01J2219/00468; B01J2219/00475; B01J2219/00497; B01J2219/005; B01J2219/00527; B01J2219/00529; B01J2219/00531; B01J2219/00583; B01J2219/00585; B01J2219/0059; B01J2219/00596; B01J2219/00603; B01J2219/00605; B01J2219/00608; B01J2219/00639; B01J2219/00641; B01J2219/00646; B01J2219/00648; B01J2219/00659; B01J2219/0068; B01J2219/00686; B01J2219/00689; B01J2219/00695; B01J2219/00709; B01J2219/00711; B01J2219/00718; B01J2219/0072; B01J2219/00722; B01J2219/00725; C07B; C07B2200/00; C07B2200/11; C40; C40B; C40B40/00; C40B40/04; C40B40/06; C40B40/10; C40B60/00; C40B60/14; G01; G01N; G01N15/00; G01N15/10; G01N15/14; G01N15/1429; G01N15/1433
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
83 words
A method of using a fluorescent label to detect hybridization with polynucleotide probes of known sequence, said method comprising: (a) attaching said label to a target polynucleotide, (b) contacting under hybridization conditions said labeled target polynucleotide sequence with a collection of polynucleotide probes of known sequences wherein said probes are attached to a substrate at known locations; and (c) determining the sequences of the probes which hybridize with said target, said collection comprising at least 100 different probes per square centimeter of substrate.
Claim 2
Independent Claim
83 words
A method of using a fluorescent label to detect hybridization with polynucleotide probes of known sequence, said method comprising: (a) attaching said label to a target polynucleotide, (b) contacting under hybridization conditions said labeled target polynucleotide sequence with a collection of polynucleotide probes of known sequences wherein said probes are attached to a substrate at known locations; and (c) determining the sequences of the probes which hybridize with said target, said collection comprising at least 100 different probes per square centimeter of substrate.
Claim 3
Independent Claim
86 words
A method of mapping a plurality of polynucleotide sequences relative to one another, said method comprising: (a) preparing a substrate having attached thereto at known locations a plurality of sequence specific probes comprising at least about 100 probes per square centimeter of substrate; (b) subjecting each of said polynucleotide sequences to contact with the probes attached to said substrate under hybridization conditions; and (c) determining the relative locations of said hybridizations on said sequences to determine the overlaps and order of said plurality of polynucleotide sequences.
Claim 4
Independent Claim
86 words
A method of mapping a plurality of polynucleotide sequences relative to one another, said method comprising: (a) preparing a substrate having attached thereto at known locations a plurality of sequence specific probes comprising at least about 100 probes per square centimeter of substrate; (b) subjecting each of said polynucleotide sequences to contact with the probes attached to said substrate under hybridization conditions; and (c) determining the relative locations of said hybridizations on said sequences to determine the overlaps and order of said plurality of polynucleotide sequences.
Claim 5
Dependent Claim
12 words
The method of claim 2, wherein said sequence specific probes are oligonucleotides.
Claim 6
Independent Claim
81 words
A method comprising the steps of: (a) hybridizing non-identical oligonucleotide probes attached to a substrate at a density of at least 100 of said probes per square centimeter with target DNA; (b) identifying a first oligonucleotide probe which hybridizes in step (a) with said target DNA; (c) further identifying other oligonucleotide probes which hybridize in step (a) beginning with a second oligonucleotide probe; and (d) continuing said identification to identify at least about 100 probes which hybridize with said target DNA.
Claim 7
Independent Claim
81 words
A method comprising the steps of: (a) hybridizing non-identical oligonucleotide probes attached to a substrate at a density of at least 100 of said probes per square centimeter with target DNA; (b) identifying a first oligonucleotide probe which hybridizes in step (a) with said target DNA; (c) further identifying other oligonucleotide probes which hybridize in step (a) beginning with a second oligonucleotide probe; and (d) continuing said identification to identify at least about 100 probes which hybridize with said target DNA.
References Cited (Backward Citations)
US4689405A
Cited
US4713326A
Cited
US4542102A
Cited
US5026840A
Cited
US4728591A
Cited
US4965188A
Cited
US5202231A
Cited
US5492806A
Cited
US5525464A
Cited
US5002867A
Cited
WO1989010977A1
Cited
US5700637A
Cited
WO1989011548A1
Cited
WO1990000626A1
Cited
WO1990003382A1
Cited
WO1990004652A1
Cited
EP0392546A2
Cited
US5143854A
Cited
US5445934A
Cited
WO1990015070A1
Cited
GB2233654A
Cited
WO1991007087A1
Cited
WO1992010092A1
Cited
WO1992010588A1
Cited
WO1993017126A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2002-02-15 | 2002-08-17 | 2003-02-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2006-02-15 | 2006-08-17 | 2007-02-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2010-02-15 | 2010-08-17 | 2011-02-16 | $7700.00 | Window Closed / Lapsed |