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Patent US5853994A successfully refreshed from Google Patents.
US5853994A
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US
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Particle agglutination assay system
Assignee: Analogic Corp
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Filing: 1996-12-05
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Granted: 1998-12-29
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Expires: 2016-12-05
Abstract
Abstract A system for assaying a fluid sample for one or more types of analytes, which employs at least one class of finely divided polystyrene spheroidal particles, each class being limited to a predetermined specific narrow range of particle diameters, the particles of each such class being coated with a specific reactant unique for that class. After the coated particles are mixed with the sample to specifically react to form conjugates of the particles and any of the analyte present, the mixture is irradiated with bursts of ultrasound swept over a range of frequencies resonant to the expected conjugate sizes. The presence of the conjugates and therefore the analyte is detected directly by measuring any selective absorption or scattering of waves of frequencies to which the conjugates are resonant.
Inventors
- 👤 Gopal Gopinathan
- 👤 Edmund F. Becker
- 👤 William Wong
- 👤 Qiang Xue
- 👤 Carl M. Good, III
- 👤 John Fallon
- 👤 Xue-Song Li
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 13:49
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/585; G01N29/00; G01N29/02; G01N29/032; G01N29/036; G01N33/53; G01N33/543; G01N33/54313; G01N2291/00; G01N2291/01; G01N2291/014; G01N2291/015; G01N2291/02; G01N2291/024; G01N2291/02416; G01N2291/02441; G01N2291/02466; G01N2291/025; G01N2291/0255; G01N2291/04; G01N2291/042; G01N2291/0421; Y; Y10; Y10S; Y10S436/00; Y10S436/806
Claims Analysis
Total Claims: 43
Claim 1
Independent Claim
348 words
Apparatus for assaying a fluid sample for an analyte, said apparatus comprising, in combination a first reagent including at least a first class of minute initial particles dimensioned within a predeterminedly narrow range of particle diameters and being coated with a reactant capable of entering into a reaction involving said analyte so as to generate resultant particles from said initial particles and of different dimensions than said initial particles, said resultant particles having characteristic acoustic scattering cross sections; at least a second class of minute, substantially spheroidal particles within a predeterminedly narrow range of substantially larger particle diameters than the diameters of said particles of said first class, the particles of said second class being coated with said reactant; and means for forming a mixture of the reactant coated particles of said first and second classes with said sample so as to initiate said reaction and form said resultant particles from said first and second classes of particles by said reaction with analyte present in said sample, said means for forming a mixture comprising a hollow flow cell having a chamber of capillary cross-section with inlet and outlet conduits thereto for respectively introducing and removing the mixture of reagent and sample to and from said chamber; means for irradiating the mixture of said reagent and sample with compressional waves in a predetermined frequency range at which said resultant particles will cause a reduction in forward energy of said waves within a wavelength band determined in accordance with the scattering cross-sections of said resultant particles; a sonic delay line disposed between said source and said mixture so as to delay the propagation of said waves irradiating said mixture; acoustic reflector means positioned for reflecting said waves back toward said source after having traversed said mixture; sensor means for detecting said reduction of said forward energy by said resultant particles, said sensor means comprising means for generating electrical signals in accordance with the detected reduction in said forward energy; and means for determining from the frequency and amplitude of said electrical signals an assay of said analyte in said sample.
Claim 2
Dependent Claim
348 words
Apparatus for assaying a fluid sample for an analyte, said apparatus comprising, in combination a first reagent including at least a first class of minute initial particles dimensioned within a predeterminedly narrow range of particle diameters and being coated with a reactant capable of entering into a reaction involving said analyte so as to generate resultant particles from said initial particles and of different dimensions than said initial particles, said resultant particles having characteristic acoustic scattering cross sections; at least a second class of minute, substantially spheroidal particles within a predeterminedly narrow range of substantially larger particle diameters than the diameters of said particles of said first class, the particles of said second class being coated with said reactant; and means for forming a mixture of the reactant coated particles of said first and second classes with said sample so as to initiate said reaction and form said resultant particles from said first and second classes of particles by said reaction with analyte present in said sample, said means for forming a mixture comprising a hollow flow cell having a chamber of capillary cross-section with inlet and outlet conduits thereto for respectively introducing and removing the mixture of reagent and sample to and from said chamber; means for irradiating the mixture of said reagent and sample with compressional waves in a predetermined frequency range at which said resultant particles will cause a reduction in forward energy of said waves within a wavelength band determined in accordance with the scattering cross-sections of said resultant particles; a sonic delay line disposed between said source and said mixture so as to delay the propagation of said waves irradiating said mixture; acoustic reflector means positioned for reflecting said waves back toward said source after having traversed said mixture; sensor means for detecting said reduction of said forward energy by said resultant particles, said sensor means comprising means for generating electrical signals in accordance with the detected reduction in said forward energy; and means for determining from the frequency and amplitude of said electrical signals an assay of said analyte in said sample.
Claim 3
Dependent Claim
231 words
Apparatus for assaying a fluid sample for an analyte, said apparatus comprising, in combination a first reagent including at least a first class of minute initial particles dimensioned within a predeterminedly narrow range of particle diameters and being coated with a reactant capable of entering into a reaction involving said analyte so as to generate resultant particles from said initial particles and of different dimensions than said initial particles, said resultant particles having characteristic acoustic scattering cross sections; at least a second class of minute, substantially spheroidal particles within a predeterminedly narrow range of substantially larger particle diameters than the diameters of said particles of said first class, the particles of said second class being coated with said reactant; and means for forming a mixture of the reactant coated particles of said first and second classes with said sample so as to initiate said reaction and form said resultant particles from said first and second classes of particles by said reaction with analyte present in said sample; and means for irradiating the mixture of said reagent and sample with compressional waves in a predetermined frequency range at which said resultant particles will cause a reduction in forward energy of said waves within a wavelength band determined in accordance with the scattering cross-sections of said resultant particles; and sensor means for detecting said reduction of said forward energy by said resultant particles.
Claim 4
Dependent Claim
231 words
Apparatus for assaying a fluid sample for an analyte, said apparatus comprising, in combination a first reagent including at least a first class of minute initial particles dimensioned within a predeterminedly narrow range of particle diameters and being coated with a reactant capable of entering into a reaction involving said analyte so as to generate resultant particles from said initial particles and of different dimensions than said initial particles, said resultant particles having characteristic acoustic scattering cross sections; at least a second class of minute, substantially spheroidal particles within a predeterminedly narrow range of substantially larger particle diameters than the diameters of said particles of said first class, the particles of said second class being coated with said reactant; and means for forming a mixture of the reactant coated particles of said first and second classes with said sample so as to initiate said reaction and form said resultant particles from said first and second classes of particles by said reaction with analyte present in said sample; and means for irradiating the mixture of said reagent and sample with compressional waves in a predetermined frequency range at which said resultant particles will cause a reduction in forward energy of said waves within a wavelength band determined in accordance with the scattering cross-sections of said resultant particles; and sensor means for detecting said reduction of said forward energy by said resultant particles.
Claim 5
Dependent Claim
73 words
Apparatus for assaying a fluid sample as set forth in claim 2 wherein said first class of said particles is selected such that they exhibit acoustic scattering substantially outside of the bandwidth of said sensor means, and the bandwidth of said sensor means is selected so as to detect acoustic scattering by both said second class of said particles and said conjugates formed by reaction among said first and second classes of particles.
Claim 6
Dependent Claim
43 words
Apparatus for assaying a fluid sample as set forth in claim 2 wherein the average diameter within the range of said second class of said particles is at least twice the average diameter within the range of said first class of said particles.
Claim 7
Dependent Claim
46 words
Apparatus for assaying a fluid sample as set forth in claim 2 wherein said particles of said first class have diameters between 0.5±μm to about 1.5±0.1 μm, and said particles of said second class have diameters between 4.5±0.1 μm to about 10 ±0.1 μm in diameter.
Claim 8
Dependent Claim
47 words
Apparatus as set forth in claim 2 wherein said first class of initial particles are linked conjugates of smaller, substantially spheroidal particles, said linked conjugates being cleaved by said reaction so as to release said spheroidal particles as resultant particles of different dimensions than said initial particles.
Claim 9
Dependent Claim
180 words
Apparatus for assaying a fluid sample for an analyte, said apparatus comprising, in combination a first reagent including at least a first class of minute initial particles dimensioned within a predeterminedly narrow range of particle diameters, said particles comprising a reactant capable of entering into a reaction involving said analyte so as to generate resultant particles of different dimensions than said initial particles, said resultant particles having characteristic acoustic scattering cross sections; means for forming a mixture of said reagent with said fluid sample so as to initiate said reaction; a source of ultrasonic waves for irradiating the mixture of said reagent and sample with said waves in a predetermined frequency range at which said resultant particles will cause a reduction in forward energy of said waves within a wavelength band determined in accordance with the scattering cross-sections of said resultant particles; an ultrasonic delay line disposed between said source and said mixture so as to delay the propagation of said waves irradiating said mixture; and sensor means for detecting said reduction of said forward energy by said resultant particles.
Claim 10
Dependent Claim
180 words
Apparatus for assaying a fluid sample for an analyte, said apparatus comprising, in combination a first reagent including at least a first class of minute initial particles dimensioned within a predeterminedly narrow range of particle diameters, said particles comprising a reactant capable of entering into a reaction involving said analyte so as to generate resultant particles of different dimensions than said initial particles, said resultant particles having characteristic acoustic scattering cross sections; means for forming a mixture of said reagent with said fluid sample so as to initiate said reaction; a source of ultrasonic waves for irradiating the mixture of said reagent and sample with said waves in a predetermined frequency range at which said resultant particles will cause a reduction in forward energy of said waves within a wavelength band determined in accordance with the scattering cross-sections of said resultant particles; an ultrasonic delay line disposed between said source and said mixture so as to delay the propagation of said waves irradiating said mixture; and sensor means for detecting said reduction of said forward energy by said resultant particles.
Claim 11
Dependent Claim
175 words
Apparatus for assaying a fluid sample for an analyte, said apparatus comprising, in combination a first reagent including at least a first class of minute initial particles dimensioned within a predeterminedly narrow range of particle diameters, said particles comprising a reactant capable of entering into a reaction involving said analyte so as to generate resultant particles of different dimensions than said initial particles, said resultant particles having characteristic acoustic scattering cross sections; means for forming a mixture of said reagent with said fluid sample so as to initiate said reaction; a source of ultrasonic waves for irradiating the mixture of said reagent and sample with said waves in a predetermined frequency range at which said resultant particles will cause a reduction in forward energy of said waves within a wavelength band determined in accordance with the scattering cross-sections of said resultant particles; acoustic reflector means positioned for reflecting said ultrasonic waves back toward said source after having traversed said mixture; and sensor means for detecting said reduction of said forward energy by said resultant particles.
Claim 12
Dependent Claim
175 words
Apparatus for assaying a fluid sample for an analyte, said apparatus comprising, in combination a first reagent including at least a first class of minute initial particles dimensioned within a predeterminedly narrow range of particle diameters, said particles comprising a reactant capable of entering into a reaction involving said analyte so as to generate resultant particles of different dimensions than said initial particles, said resultant particles having characteristic acoustic scattering cross sections; means for forming a mixture of said reagent with said fluid sample so as to initiate said reaction; a source of ultrasonic waves for irradiating the mixture of said reagent and sample with said waves in a predetermined frequency range at which said resultant particles will cause a reduction in forward energy of said waves within a wavelength band determined in accordance with the scattering cross-sections of said resultant particles; acoustic reflector means positioned for reflecting said ultrasonic waves back toward said source after having traversed said mixture; and sensor means for detecting said reduction of said forward energy by said resultant particles.
Claim 13
Dependent Claim
42 words
Apparatus for assaying a fluid sample as set forth in claim 8 wherein said acoustic reflector means is aligned so that the reflected waves are reflected back toward said source in a direction substantially parallel to the incident waves from said source.
Claim 14
Dependent Claim
25 words
Apparatus for assaying a fluid sample as set forth in claim 9 wherein said acoustic reflector means is shaped substantially as a 90° hollow cone.
Claim 15
Dependent Claim
196 words
Apparatus for assaying a fluid sample for an analyte, said apparatus comprising, in combination a first reagent including at least a first class of minute initial particles dimensioned within a predeterminedly narrow range of particle diameters, said particles comprising a reactant capable of entering into a reaction involving said analyte so as to generate resultant particles of different dimensions than said initial particles, said resultant particles having characteristic acoustic scattering cross sections; means for forming a mixture of said reagent with said fluid sample so as to initiate said reaction, said means for forming a mixture comprising a hollow flow cell having a chamber of capillary cross-section with inlet and outlet conduits thereto for respectively introducing and removing the mixture of reagent and sample to and from said chamber; a source of ultrasonic waves for irradiating the mixture of said reagent and sample with said waves in a predetermined frequency range at which said resultant particles will cause a reduction in forward energy of said waves within a wavelength band determined in accordance with the scattering cross-sections of said resultant particles; and sensor means for detecting said reduction of said forward energy by said resultant particles.
Claim 16
Dependent Claim
196 words
Apparatus for assaying a fluid sample for an analyte, said apparatus comprising, in combination a first reagent including at least a first class of minute initial particles dimensioned within a predeterminedly narrow range of particle diameters, said particles comprising a reactant capable of entering into a reaction involving said analyte so as to generate resultant particles of different dimensions than said initial particles, said resultant particles having characteristic acoustic scattering cross sections; means for forming a mixture of said reagent with said fluid sample so as to initiate said reaction, said means for forming a mixture comprising a hollow flow cell having a chamber of capillary cross-section with inlet and outlet conduits thereto for respectively introducing and removing the mixture of reagent and sample to and from said chamber; a source of ultrasonic waves for irradiating the mixture of said reagent and sample with said waves in a predetermined frequency range at which said resultant particles will cause a reduction in forward energy of said waves within a wavelength band determined in accordance with the scattering cross-sections of said resultant particles; and sensor means for detecting said reduction of said forward energy by said resultant particles.
Claim 17
Dependent Claim
57 words
Apparatus for assaying a fluid sample as set forth in claim 11 wherein said means for irradiating said resultant particles with compressional waves comprises an acoustic transducer mounted adjacent one side of said chamber so that the direction of acoustic propagation from said transducer is directed substantially normally to the direction of fluid flow through said chamber.
Claim 18
Dependent Claim
37 words
Apparatus for assaying a fluid sample as set forth in claim 11 including an air vent coupled to said chamber, the cross-section dimension of said vent being substantially less than the cross-section dimension of said outlet conduit.
Claim 19
Dependent Claim
205 words
Apparatus for assaying a fluid sample for an analyte, said apparatus comprising, in combination a first reagent including at least a first class of minute initial particles dimensioned within a predeterminedly narrow range of particle diameters, said particles comprising a reactant capable of entering into a reaction involving said analyte so as to generate resultant particles of different dimensions than said initial particles, said resultant particles having characteristic acoustic scattering cross sections; means for forming a mixture of said reagent with said fluid sample so as to initiate said reaction, means for irradiating the mixture of said reagent and sample with compressional waves in a predetermined frequency range at which said resultant particles will cause a reduction in forward energy of said waves within a wavelength band determined in accordance with the scattering cross-sections of said resultant particles, said means for irradiating comprising acoustic transducer means for providing said compressional waves; sensor means for detecting said reduction of said forward energy by said resultant particles, said sensor means comprising means for generating electrical signals in accordance with the detected reduction in said forward energy; and means for determining from the frequency and amplitude of said electrical signals an assay of said analyte in said sample.
Claim 20
Dependent Claim
205 words
Apparatus for assaying a fluid sample for an analyte, said apparatus comprising, in combination a first reagent including at least a first class of minute initial particles dimensioned within a predeterminedly narrow range of particle diameters, said particles comprising a reactant capable of entering into a reaction involving said analyte so as to generate resultant particles of different dimensions than said initial particles, said resultant particles having characteristic acoustic scattering cross sections; means for forming a mixture of said reagent with said fluid sample so as to initiate said reaction, means for irradiating the mixture of said reagent and sample with compressional waves in a predetermined frequency range at which said resultant particles will cause a reduction in forward energy of said waves within a wavelength band determined in accordance with the scattering cross-sections of said resultant particles, said means for irradiating comprising acoustic transducer means for providing said compressional waves; sensor means for detecting said reduction of said forward energy by said resultant particles, said sensor means comprising means for generating electrical signals in accordance with the detected reduction in said forward energy; and means for determining from the frequency and amplitude of said electrical signals an assay of said analyte in said sample.
Claim 21
Dependent Claim
45 words
Apparatus for assaying a fluid sample as set forth in claim 14 wherein said transducer means comprises a sonic generator for providing said compressional waves and also includes said sensor means for detecting said change in said forward energy and for producing said electrical signals.
Claim 22
Dependent Claim
27 words
Apparatus for assaying a fluid sample as set forth in claim 14 wherein said transducer means is operable to produce said compressional waves as a continuous excitation.
Claim 23
Dependent Claim
20 words
Apparatus for assaying a fluid sample as set forth in claim 16 wherein said excitation is at a single frequency.
Claim 24
Dependent Claim
33 words
Apparatus for assaying a fluid sample as set forth in claim 16 wherein said excitation is continuously varied in a sawtooth or triangular format to produce a spectrum as a function of time.
Claim 25
Dependent Claim
36 words
Apparatus for assaying a fluid sample as set forth in claim 16 wherein said excitation is a continuous signal composed of a superposition of individual signals of single frequencies, each corresponding to a wavelength of interest.
Claim 26
Dependent Claim
32 words
Apparatus for assaying a fluid sample as set forth in claim 16 wherein said transducer means is operable to produce said compressional waves as a plurality of sonic pulses having wideband spectra.
Claim 27
Dependent Claim
50 words
Apparatus for assaying a fluid sample as set forth in claim 20 wherein said transducer means includes means for introducing a delay into the propagation of said pulses through said mixture sufficient to permit said transducer means to switch between transmission and reception of the energy of the compressional waves.
Claim 28
Dependent Claim
98 words
Apparatus for assaying a fluid sample as set forth in claim 20 including: a flow cell defining a hollow, capillary flow channel having inlet and outlet ends, said channel being directed substantially normally to the direction of acoustic propagation from said transducer means; acoustic reflector means positioned so that said channel is disposed between said transducer means and said reflector means; a narrow, vertical, open air vent having an internal diameter much smaller than the diameter of said channel and disposed adjacent said outlet end of said channel for coupling the outside of said cell with said channel.
Claim 29
Dependent Claim
25 words
Apparatus for assaying a fluid sample as set forth in claim 22 wherein the cross-section dimension of said channel is several thousandths of an inch.
Claim 30
Dependent Claim
46 words
Apparatus for assaying a fluid sample as set forth in claim 22 wherein said acoustic reflector means is aligned to reflect said compressional waves back toward said transducer means within about 1 angular degree of the direction of propagation of said waves from said transducer means.
Claim 31
Dependent Claim
46 words
Apparatus for assaying a fluid sample as set forth in claim 22 wherein said acoustic reflector means is configured as a hollow 90° cone which provides the property of returning the compressional wave reflected therefrom in a direction substantially parallel to the compressional wave incident thereon.
Claim 32
Dependent Claim
176 words
Apparatus for assaying a fluid sample as set forth in claim 14 wherein said means for determining the frequency and amplitude of said electrical signals comprises: means for amplifying said electrical signals; voltage controlled oscillator means for producing a sinusoidal signal; mixer means for multiplying the amplified signals with said sinusoidal signal from said voltage controlled oscillator means so as to produce multiplied signals; filter means for rejecting signal components in said multiplied signals so as to produce corresponding low frequency signals; means for detecting said low frequency signals; sample-and-hold circuit means for storing said low frequency signals; means coupled to the output of said sample-and-hold circuit means for continuously compared the signals stored in said sample-and-hold circuit means with signal common to produce error signals; means for applying said error signals to said voltage-controlled oscillator means so as to control the frequency of the latter, said error signals providing a measure of the frequency of said change of said forward energy, the signals stored in said sample-and-hold circuit means representing the amplitude of said change.
Claim 33
Dependent Claim
40 words
Apparatus for assaying a fluid sample as set forth in claim 26 wherein said filter means is a bandpass filter; said means for detecting said low frequency signals is a detector; and said sample-and-hold circuit means is a sample-and-hold circuit.
Claim 34
Dependent Claim
46 words
Apparatus for assaying a fluid sample as set forth in claim 26 wherein said filter means comprises a plurality of bandpass filters; said means for detecting said low frequency signals comprises a plurality of detectors; and said sample-and-hold circuit means comprises a plurality of sample-and-hold circuits.
Claim 35
Dependent Claim
210 words
Method of assaying for at least one type of analyte in a liquid sample, including the steps of providing a first reagent including at least a first class of minute particles dimensioned within a predeterminedly narrow range of particle diameters and being coated with a reactant capable of entering into a reaction involving said analyte so as to generate resultant particles of different dimensions than said first class of initial particles, said resultant particles having characteristic acoustic scattering cross sections; providing a second reagent including at least a second class of substantially larger particle diameters than the diameters of said particles of said first class, the particles of said second class being coated with said first reactant; forming a mixture of the reactant coated particles of said first and second classes with said sample so as to effect a specific reaction involving said reactant and said analyte to form conjugates of said first and second classes of particles, said conjugates having a characteristic acoustic scattering cross-sections; irradiating the conjugates with compressional waves in a predetermined frequency range at which said conjugates will cause a reduction in forward energy of said waves within a wavelength band determined in accordance with said scattering cross-sections; and detecting said reduction of said forward energy.
Claim 36
Dependent Claim
210 words
Method of assaying for at least one type of analyte in a liquid sample, including the steps of providing a first reagent including at least a first class of minute particles dimensioned within a predeterminedly narrow range of particle diameters and being coated with a reactant capable of entering into a reaction involving said analyte so as to generate resultant particles of different dimensions than said first class of initial particles, said resultant particles having characteristic acoustic scattering cross sections; providing a second reagent including at least a second class of substantially larger particle diameters than the diameters of said particles of said first class, the particles of said second class being coated with said first reactant; forming a mixture of the reactant coated particles of said first and second classes with said sample so as to effect a specific reaction involving said reactant and said analyte to form conjugates of said first and second classes of particles, said conjugates having a characteristic acoustic scattering cross-sections; irradiating the conjugates with compressional waves in a predetermined frequency range at which said conjugates will cause a reduction in forward energy of said waves within a wavelength band determined in accordance with said scattering cross-sections; and detecting said reduction of said forward energy.
Claim 37
Dependent Claim
81 words
In a method as defined in claim 29 including the steps of coating a plurality of particles of a plurality of said classes with a reactant unique to each such class and couplable with a different analyte in said sample, and mixing said reactant coated particles of each of said classes with said sample so as to effect a specific reaction involving each of said reactants and the corresponding one of said different analytes to form respective conjugates of said particles.
Claim 38
Dependent Claim
56 words
In a method as defined in claim 29 including the steps of coating a plurality of particles of each of a plurality of said classes with a plurality of types of reactant unique to that class, each of said plurality of types of reactant being couplable with a distinct analyte so as to form corresponding conjugates.
Claim 39
Dependent Claim
60 words
In a method as defined in claim 29 wherein said initial particles of said first class are selected as minute, substantially spheroidal particles coated with a reactant capable of entering into a binding reaction with high specificity with said analyte to form resultant particles that are conjugates of said initial particles; and said change in forward energy is a reduction.
Claim 40
Dependent Claim
46 words
In a method as defined in claim 29 wherein said initial particles of said first class are selected as linked conjugates of smaller particles reactable with said analyte to cleave the linkages in said conjugates so as to form resultant particles that are said smaller particles.
Claim 41
Dependent Claim
106 words
In a method as defined in claim 29 including the steps of providing a plurality of up to four of said classes of finely divided, substantially spheroidal particles, each of said classes being limited to a unique, predeterminedly narrow range of particle diameters; coating the particles of each of said classes coated with a corresponding, different reactant capable of entering into a reaction with high specificity; and forming a simultaneous mixture of said reagent coated particles with said sample so as to incite a plurality of specific reactions each involving one of said reagents and a corresponding type of analyte to form conjugates of said particles.
Claim 42
Dependent Claim
72 words
In a method as defined in claim 29, including the steps of introducing said compressional waves in said frequency range into a fluid free of said resultant particles so as to determine the forward energy of said waves propagated through said fluid; and comparing the forward energy of said waves propagated through said fluid with said change in forward energy in said waves propagated through said mixture of reagent and fluid sample.
Claim 43
Dependent Claim
15 words
In a method as defined in claim 29 wherein said liquid sample is whole blood.
References Cited (Backward Citations)
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| 7.5 Years (Year 8) | 2005-12-28 | 2006-06-29 | 2006-12-29 | $3760.00 | Window Closed / Lapsed |
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