US4615984A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Dissociation of ligand-binder complex using ultrasound
Assignee: Becton Dickinson and Co
•
Filing: 1984-02-23
•
Granted: 1986-10-07
•
Expires: 2004-02-23
Abstract
Abstract A ligand is dissociated from a binder specific for the ligand by directing a beam of ultrasound at the complex of ligand and binder. The procedure may be employed in a solid phase assay to separate a labeled form of ligand, employed as a tracer, from a binder supported on a solid support.
Inventors
- 👤 Ronald L. Stoker
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 11:31
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; Y; Y10; Y10S; Y10S436/00; Y10S436/824
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
51 words
A process for dissociating a complex of a ligand bound to a binder specific for the ligand, comprising: directing a beam of ultrasound at a complex of a ligand bound to a binder specific for the ligand for a time and under conditions effective to separate the ligand from the binder.
Claim 2
Dependent Claim
51 words
A process for dissociating a complex of a ligand bound to a binder specific for the ligand, comprising: directing a beam of ultrasound at a complex of a ligand bound to a binder specific for the ligand for a time and under conditions effective to separate the ligand from the binder.
Claim 3
Dependent Claim
28 words
The process of claim 1 wherein the complex is in contact with a liquid at the time of said directing of said beam of ultrasound at the complex.
Claim 4
Dependent Claim
14 words
The process of claim 2 wherein the binder is supported on a solid support.
Claim 5
Dependent Claim
12 words
The process of claim 3 wherein said liquid is an eluting liquid.
Claim 6
Dependent Claim
36 words
The process of claim 4 wherein the binder is supported on a solid support in a flowthrough chamber and the beam of ultrasound is directed against the binder while an eluting liquid flows through the chamber.
Claim 7
Dependent Claim
11 words
The process of claim 5 wherein the binder is an antibody.
Claim 8
Dependent Claim
27 words
The process of claim 6 wherein at least a portion of the ligand which is bound to the binder is a ligand labeled with a detectable marker.
Claim 9
Dependent Claim
89 words
In an assay wherein a tracer comprising a labeled form of a ligand is bound to a binder specific for the ligand on a solid support to form a complex of tracer and binder on the solid support, the improvement comprising: directing a beam of ultrasound at a complex of a tracer comprising a labeled form of a ligand bound to a binder specific for the ligand on a solid support for a time and under conditions effective to separate the tracer from the binder of the solid support.
Claim 10
Dependent Claim
89 words
In an assay wherein a tracer comprising a labeled form of a ligand is bound to a binder specific for the ligand on a solid support to form a complex of tracer and binder on the solid support, the improvement comprising: directing a beam of ultrasound at a complex of a tracer comprising a labeled form of a ligand bound to a binder specific for the ligand on a solid support for a time and under conditions effective to separate the tracer from the binder of the solid support.
Claim 11
Dependent Claim
26 words
The process of claim 8 wherein simultaneously with the directing of said beam of ultrasound at the complex, said complex is in contact with a liquid.
Claim 12
Dependent Claim
20 words
The process of claim 9 wherein said beam of ultrasound has a frequency of at least 20,000 cycles per second.
Claim 13
Dependent Claim
35 words
The process of claim 10 wherein said binder is supported on a solid support in a flowthrough chamber and the beam of ultrasound is directed against the binder while a liquid flows through the chamber.
Claim 14
Dependent Claim
37 words
The process of claim 11 wherein said liquid is an eluting liquid and the intensity of the beam of ultrasound is controlled to prevent destruction of the binder whereby the binder may be reused in the assay.
Claim 15
Dependent Claim
11 words
The process of claim 12 wherein the binder is an antibody.
Claim 16
Independent Claim
38 words
An apparatus, comprising: flowthrough chamber; a binder, for specifically binding a ligand, supported on a solid support in said chamber; and ultrasonic means connected to said chamber for producing and directing a beam of ultrasound against said binder.
Claim 17
Independent Claim
38 words
An apparatus, comprising: flowthrough chamber; a binder, for specifically binding a ligand, supported on a solid support in said chamber; and ultrasonic means connected to said chamber for producing and directing a beam of ultrasound against said binder.
References Cited (Backward Citations)
US3003707A
Cited
GB1015962A
Cited
US3492212A
Cited
US3550586A
Cited
GB1303823A
Cited
US4059685A
Cited
US3825481A
Cited
US4180383A
Cited
US4261828A
Cited
US4389253A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-10-06 | 1990-04-07 | 1990-10-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-10-06 | 1994-04-07 | 1994-10-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-10-06 | 1998-04-07 | 1998-10-07 | $7700.00 | Window Closed / Lapsed |