US5755953A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Interference free biosensor
Assignee: Abbott Laboratories
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Filing: 1995-12-18
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Granted: 1998-05-26
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Expires: 2015-12-18
Abstract
Abstract Provided are microparticle forms of carbon, carbon catalysts and carbon-containing electrically conductive materials which are covalently linked to peroxidase. The carbon:peroxidase conjugates are suitable for use as substrates in conventional electrodes. Surprisingly, the conjugates display very little sensitivity to known interfering substances and thus are suitable for use as interference free electrodes.
Inventors
- 👤 Timothy P. Henning
- 👤 Thomas G. Spring
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 11:12
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/002; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
89 words
A method of measuring an analyte of interest in a test sample, said method comprising the steps of (1) contacting the sample with fine particles of carbon selected from the group consisting of native carbon, derivatized carbon, and carbon-containing conductive compounds, said fine particles of carbon covalently linked to peroxidase to form a conjugate, wherein the conjugate of carbon and peroxidase is confined to an electrically conductive surface, and (2) detecting change in electrical signal as a quantitative or qualitative measure of the amount of analyte in the sample.
Claim 2
Independent Claim
89 words
A method of measuring an analyte of interest in a test sample, said method comprising the steps of (1) contacting the sample with fine particles of carbon selected from the group consisting of native carbon, derivatized carbon, and carbon-containing conductive compounds, said fine particles of carbon covalently linked to peroxidase to form a conjugate, wherein the conjugate of carbon and peroxidase is confined to an electrically conductive surface, and (2) detecting change in electrical signal as a quantitative or qualitative measure of the amount of analyte in the sample.
Claim 3
Dependent Claim
11 words
The method of claim 1 wherein the carbon is native carbon.
Claim 4
Dependent Claim
10 words
The method of claim 2 wherein the peroxidase is microperoxidase.
Claim 5
Dependent Claim
18 words
The method of claim 1 wherein the conjugate of carbon and peroxidase is confined in a resin matrix.
Claim 6
Dependent Claim
12 words
The method of claim 1 wherein the analyte is glucose or lactose.
Claim 7
Dependent Claim
19 words
The method of claim 1 wherein the carbon is derivatized carbon that has been derivatized with a bifunctional reagent.
Claim 8
Dependent Claim
30 words
A composition comprising fine particles of carbon selected from the group consisting of native carbon, derivatized carbon, and carbon-containing conductive compounds, said fine particles of carbon covalently linked to peroxidase.
Claim 9
Dependent Claim
30 words
A composition comprising fine particles of carbon selected from the group consisting of native carbon, derivatized carbon, and carbon-containing conductive compounds, said fine particles of carbon covalently linked to peroxidase.
Claim 10
Dependent Claim
11 words
The composition of claim 7 wherein the carbon is native carbon.
Claim 11
Dependent Claim
19 words
The composition of claim 7 wherein the carbon is derivatized carbon that has been derivatized with a bifunctional reagent.
Claim 12
Dependent Claim
12 words
The composition of claim 9 wherein the bifunctional reagent is a diamine.
Claim 13
Dependent Claim
12 words
The composition of claim 10 wherein the diamine is a poly(oxyalkylene) diamine.
Claim 14
Dependent Claim
10 words
The composition of claim 7 wherein the peroxidase is microperoxidase.
Claim 15
Dependent Claim
11 words
The composition of claim 12 wherein the carbon is native carbon.
Claim 16
Dependent Claim
28 words
The composition of claim 7 wherein the metal moiety of the peroxidase is modified to contain a member selected from the group consisting of iron, nickel, and zinc.
Claim 17
Dependent Claim
10 words
The composition of claim 7 further comprising a resin matrix.
Claim 18
Dependent Claim
51 words
An electrode for the determination of an analyte in a sample comprising: fine particles of carbon selected from the group consisting of native carbon, derivatized carbon, and carbon-containing conductive compounds, said fine particles of carbon covalently linked to peroxidase to form a conjugate, said conjugate confined to an electrically conductive surface.
Claim 19
Dependent Claim
51 words
An electrode for the determination of an analyte in a sample comprising: fine particles of carbon selected from the group consisting of native carbon, derivatized carbon, and carbon-containing conductive compounds, said fine particles of carbon covalently linked to peroxidase to form a conjugate, said conjugate confined to an electrically conductive surface.
Claim 20
Dependent Claim
11 words
The electrode of claim 16 wherein the carbon is native carbon.
Claim 21
Dependent Claim
10 words
The electrode of claim 17 wherein the peroxidase is microperoxidase.
Claim 22
Dependent Claim
18 words
The electrode of claim 18 wherein the conjugate of carbon and peroxidase is confined in a resin matrix.
Claim 23
Dependent Claim
19 words
The electrode of claim 16 wherein the carbon is derivatized carbon that has been derivatized with a bifunctional reagent.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-05-25 | 2001-11-24 | 2002-05-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-05-25 | 2005-11-24 | 2006-05-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-05-25 | 2009-11-24 | 2010-05-26 | $7700.00 | Window Closed / Lapsed |