US5733739A
Expired / Lapsed
US
⏱️ 0.0 years remaining
System and method for diagnosis of disease by infrared analysis of human tissues and cells
Assignee: InPhoCyte Inc
•
Filing: 1995-06-07
•
Granted: 1998-03-31
•
Expires: 2015-06-07
Abstract
Abstract A machine-based method for collecting and interpreting quantitative data on cells and tissues so that a diagnosis will obtain as to the existence or non-existence of disease in an human. Vibrational spectroscopy is used and the spectra generated by such spectroscopy are compared with stored spectra to provide whether cells or tissues are diseased, and if diseased to what degree. It, therefore, is possible to provide a basis for immediate diagnostic decisions for patients and physicians, leading in turn to immediate implementation of next-step procedures and treatment all in one visit to the doctor's office. This means that patients and the examining clinician can know almost instantly whether or not the cells or tissue examined are normal or diseased, and the level of disease present if found. The advantages of bringing the diagnostic pathology service directly into the doctor's office include immediate relief to the patient's concern about health as well as immediate clarification of what needs to be done next in order to treat the disease that is present, and include any other actions that are necessary. The need for biopsies to obtain desired information on the existence or non-existence of disease is obviated. That is, them is a rapid identification of the area of tissue that is affected by disease prior to obtaining biopsies. This makes it possible to apply a single standard of diagnostic accuracy anywhere in the world, independent of the local availability of pathologists or other professionals.
Inventors
- 👤 David S. Zakim
- 👤 John B. Lord
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 08:56
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/17; G01N21/25; G01N21/31; G01N21/35; G01N21/359; Y; Y10; Y10S; Y10S435/00; Y10S435/968; Y10T; Y10T436/00; Y10T436/24; Y10T436/25
Claims Analysis
Total Claims: 203
Claim 1
Independent Claim
107 words
A method for detecting and diagnosing disease in cells, comprising the steps of: obtaining and storing in storage means first spectra in a predetermined frequency range for at least one cell that has been determined to be free of dysplasia; collecting and distributing cells in a predetermined manner on a predetermined surface with cell collecting means; generating second spectra with for at least one cell disposed on the predetermined surface with a spectrometer means; comparing the first and second spectra for variations in frequency bands for indicating dysplasia; diagnosing a level of dysplasia based on a number and magnitude of variations of the first and second spectra.
Claim 2
Independent Claim
107 words
A method for detecting and diagnosing disease in cells, comprising the steps of: obtaining and storing in storage means first spectra in a predetermined frequency range for at least one cell that has been determined to be free of dysplasia; collecting and distributing cells in a predetermined manner on a predetermined surface with cell collecting means; generating second spectra with for at least one cell disposed on the predetermined surface with a spectrometer means; comparing the first and second spectra for variations in frequency bands for indicating dysplasia; diagnosing a level of dysplasia based on a number and magnitude of variations of the first and second spectra.
Claim 3
Dependent Claim
25 words
The method as recited in claim 1, wherein the collecting and distributing step includes collecting and distributing cells on a window of a sample holder.
Claim 4
Dependent Claim
20 words
The method as recited in claim 2, wherein the window and sample holder are transparent to infrared and Raman energies.
Claim 5
Dependent Claim
18 words
The method as recited in claim 1, wherein the generating step includes generating spectra based on vibrational spectroscopy.
Claim 6
Dependent Claim
13 words
The method as recited in claim 4, wherein the spectroscopy includes infrared spectroscopy.
Claim 7
Independent Claim
122 words
A method for of detecting and diagnosing disease in epithelial cells, comprising the steps of: (a) obtaining and storing in storage means first spectra in a mid-infrared frequency range for at least one cell from a patient being tested that has been determined to have a specific predetermined state; (b) collecting cells from the patient being tested; (c) generating second spectra in the mid-infrared frequency range from the cells collected at step (b); (d) comparing the first and second spectra for variations caused by a predetermined condition: and (e) diagnosing a change of levels of dysplasia based on the comparison at step (d) for determining a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 8
Independent Claim
122 words
A method for of detecting and diagnosing disease in epithelial cells, comprising the steps of: (a) obtaining and storing in storage means first spectra in a mid-infrared frequency range for at least one cell from a patient being tested that has been determined to have a specific predetermined state; (b) collecting cells from the patient being tested; (c) generating second spectra in the mid-infrared frequency range from the cells collected at step (b); (d) comparing the first and second spectra for variations caused by a predetermined condition: and (e) diagnosing a change of levels of dysplasia based on the comparison at step (d) for determining a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 9
Dependent Claim
18 words
The method as recited in claim 6, wherein the mid-infrared frequency range is between 600-4000 cm -1 .
Claim 10
Dependent Claim
18 words
The method as recited in claim 6, wherein the collecting at step (b) is carried out by scraping.
Claim 11
Dependent Claim
19 words
The method as recited in claim 6, wherein the collecting at step (b) is carried out by surgical biopsy.
Claim 12
Dependent Claim
20 words
The method as recited in claim 6, wherein the collecting at step (b) is carried out by fine needle aspiration.
Claim 13
Dependent Claim
21 words
The method as recited in claim 6, wherein the collecting at step (b) is carried out by a collection of stool.
Claim 14
Dependent Claim
21 words
The method as recited in claim 6, wherein the collecting at step (b) is carried out by a collection of urine.
Claim 15
Dependent Claim
21 words
The method as recited in claim 6, wherein obtaining first spectra in step (a) is carried out using infrared transmission techniques.
Claim 16
Dependent Claim
21 words
The method as recited in claim 6, wherein obtaining first spectra in step (a) is carried out using infrared reflectance techniques.
Claim 17
Dependent Claim
24 words
The method as recited in claim 6, wherein obtaining first spectra in step (a) is carried out using infrared attenuated total reflectance (ATR) techniques.
Claim 18
Dependent Claim
22 words
The method as recited in claim 6, wherein obtaining first spectra in step (a) is carried out using multi-variable perturbation infrared techniques.
Claim 19
Dependent Claim
21 words
The method as recited in claim 6, wherein generating second spectra in step (c) is carried out using infrared transmission techniques.
Claim 20
Dependent Claim
21 words
The method as recited in claim 6, wherein generating second spectra in step (c) is carried out using infrared reflectance techniques.
Claim 21
Dependent Claim
24 words
The method as recited in claim 6, wherein generating second spectra in step (c) is carried out using infrared attenuated total reflectance (ATR) techniques.
Claim 22
Dependent Claim
22 words
The method as recited in claim 6, wherein generating second spectra in step (c) is carried out using multi-variable perturbation infrared techniques.
Claim 23
Dependent Claim
22 words
The method as recited in claim 6, wherein the method is used for detecting and diagnosing disease in epithelial cells in humans.
Claim 24
Dependent Claim
22 words
The method as recited in claim 6, wherein the method is used for detecting and diagnosing disease in epithelial cells in non-humans.
Claim 25
Dependent Claim
22 words
The method as recited in claim 22, wherein the method is used for detecting and diagnosing disease in epithelial cells in animals.
Claim 26
Dependent Claim
21 words
The method as recited in claim 6, wherein the method is used for detecting and diagnosing disease in epithelial cells in-vitro.
Claim 27
Dependent Claim
17 words
The method as recited in claim 6, wherein the specific predetermined state in step (a) is dysplasia.
Claim 28
Dependent Claim
17 words
The method as recited in claim 6, wherein the specific predetermined state in step (a) is non-dysplasia.
Claim 29
Dependent Claim
17 words
The method as recited in claim 6, wherein the specific predetermined state in step (a) is precancer.
Claim 30
Dependent Claim
18 words
The method as recited in claim 6, wherein the specific predetermined state in step (a) is frank cancer.
Claim 31
Dependent Claim
16 words
The method as recited in claim 6, wherein the predetermined condition in step (d) is dysplasia.
Claim 32
Dependent Claim
16 words
The method as recited in claim 6, wherein the predetermined condition in step (d) is inflammation.
Claim 33
Dependent Claim
20 words
The method as recited in claim 6, wherein the obtaining the first Raman spectra includes collecting said first spectra in-situ.
Claim 34
Dependent Claim
19 words
The method as recited in claim 31, wherein collecting said first spectra in-situ includes collecting with a sensing probe.
Claim 35
Independent Claim
123 words
A method for detecting and diagnosing disease in epithelial cells of humans, comprising the steps of: (a) obtaining and storing in storage means first spectra in a near-infrared frequency range for at least one cell from a patient being tested that has been determined to have a specific predetermined state; (b) collecting cells from the patient being tested; (c) generating second spectra in the near-infrared frequency range from the cells collected at step (b); (d) comparing the first and second spectra for variations caused by a predetermined condition: and (e) diagnosing a change of levels of dysplasia based on the comparison at step (d) for determining a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 36
Independent Claim
123 words
A method for detecting and diagnosing disease in epithelial cells of humans, comprising the steps of: (a) obtaining and storing in storage means first spectra in a near-infrared frequency range for at least one cell from a patient being tested that has been determined to have a specific predetermined state; (b) collecting cells from the patient being tested; (c) generating second spectra in the near-infrared frequency range from the cells collected at step (b); (d) comparing the first and second spectra for variations caused by a predetermined condition: and (e) diagnosing a change of levels of dysplasia based on the comparison at step (d) for determining a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 37
Dependent Claim
18 words
The method as recited in claim 33, wherein the near-infrared frequency range is between 4000-10,000 cm -1 .
Claim 38
Dependent Claim
19 words
The method as recited in claim 33, wherein the collecting at step (b) may be carried out by scraping.
Claim 39
Dependent Claim
19 words
The method as recited in claim 33, wherein the collecting at step (b) is carrier out by surgical biopsy.
Claim 40
Dependent Claim
20 words
The method as recited in claim 33, wherein the collecting at step (b) is carried out by fine needle aspiration.
Claim 41
Dependent Claim
21 words
The method as recited in claim 33, wherein the collecting at step (b) is carried out by a collection of stool.
Claim 42
Dependent Claim
21 words
The method as recited in claim 33, wherein the collecting at step (b) is carried out by a collection of urine.
Claim 43
Dependent Claim
21 words
The method as recited in claim 33, wherein obtaining first spectra in step (a) is carried out using infrared transmission techniques.
Claim 44
Dependent Claim
21 words
The method as recited in claim 33, wherein obtaining first spectra in step (a) is carried out using infrared reflectance techniques.
Claim 45
Dependent Claim
24 words
The method as recited in claim 33, wherein obtaining first spectra in step (a) is carried out using infrared attenuated total reflectance (ATR) techniques.
Claim 46
Dependent Claim
22 words
The method as recited in claim 33, wherein obtaining first spectra in step (a) is carried out using multi-variable perturbation infrared techniques.
Claim 47
Dependent Claim
21 words
The method as recited in claim 33, wherein generating second spectra in step (c) is carried out using infrared transmission techniques.
Claim 48
Dependent Claim
21 words
The method as recited in claim 33, wherein generating second spectra in step (c) is carried out using infrared reflectance techniques.
Claim 49
Dependent Claim
24 words
The method as recited in claim 33, wherein generating second spectra in step (c) is carried out using infrared attenuated total reflectance (ATR) techniques.
Claim 50
Dependent Claim
22 words
The method as recited in claim 33, wherein generating second spectra in step (c) is carried out using multi-variable perturbation infrared techniques.
Claim 51
Dependent Claim
21 words
The method as recited in claim 33, wherein the method is used for detecting and diagnosing disease in epithelial cells in-vitro.
Claim 52
Dependent Claim
17 words
The method as recited in claim 33, wherein the specific predetermined state in step (a) is dysplasia.
Claim 53
Dependent Claim
17 words
The method as recited in claim 33, wherein the specific predetermined state in step (a) is non-dysplasia.
Claim 54
Dependent Claim
17 words
The method as recited in claim 33, wherein the specific predetermined state in step (a) is precancer.
Claim 55
Dependent Claim
18 words
The method as recited in claim 33, wherein the specific predetermined state in step (a) is frank cancer.
Claim 56
Dependent Claim
16 words
The method as recited in claim 33, wherein the predetermined condition in step (d) is dysplasia.
Claim 57
Dependent Claim
16 words
The method as recited in claim 33, wherein the predetermined condition in step (d) is inflammation.
Claim 58
Dependent Claim
20 words
The method as recited in claim 33, wherein the obtaining the first Raman spectra includes collecting said first spectra in-situ.
Claim 59
Dependent Claim
19 words
The method as recited in claim 55, wherein collecting said first spectra in-situ includes collecting with a sensing probe.
Claim 60
Independent Claim
115 words
A method for of detecting and diagnosing disease in epithelial cells, comprising the steps of: (a) obtaining and storing in storage means first Raman spectra for at least one cell from a patient being tested that has been determined to have a specific predetermined state; (b) collecting cells from the patient being tested; (c) generating second Raman spectra from the cells collected at step (b); (d) comparing the first and second Raman spectra for variations caused by a predetermined condition: and (e) diagnosing a change of levels of dysplasia based on the comparison at step (d) for determining a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 61
Independent Claim
115 words
A method for of detecting and diagnosing disease in epithelial cells, comprising the steps of: (a) obtaining and storing in storage means first Raman spectra for at least one cell from a patient being tested that has been determined to have a specific predetermined state; (b) collecting cells from the patient being tested; (c) generating second Raman spectra from the cells collected at step (b); (d) comparing the first and second Raman spectra for variations caused by a predetermined condition: and (e) diagnosing a change of levels of dysplasia based on the comparison at step (d) for determining a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 62
Dependent Claim
18 words
The method as recited in claim 57, wherein the collecting at step (b) is carrier out by scraping.
Claim 63
Dependent Claim
19 words
The method as recited in claim 57, wherein the collecting at step (b) is carried out by surgical biopsy.
Claim 64
Dependent Claim
20 words
The method as recited in claim 57, wherein the collecting at step (b) is carried out by fine needle aspiration.
Claim 65
Dependent Claim
21 words
The method as recited in claim 57, wherein the collecting at step (b) is carried out by a collection of stool.
Claim 66
Dependent Claim
21 words
The method as recited in claim 57, wherein the collecting at step (b) is carried out by a collection of urine.
Claim 67
Dependent Claim
22 words
The method as recited in claim 57, wherein the method is used for detecting and diagnosing disease in epithelial cells in humans.
Claim 68
Dependent Claim
22 words
The method as recited in claim 57, wherein the method is used for detecting and diagnosing disease in epithelial cells in non-humans.
Claim 69
Dependent Claim
22 words
The method as recited in claim 64, wherein the method is used for detecting and diagnosing disease in epithelial cells in animals.
Claim 70
Dependent Claim
21 words
The method as recited in claim 57, wherein the method is used for detecting and diagnosing disease in epithelial cells in-vitro.
Claim 71
Dependent Claim
17 words
The method as recited in claim 57, wherein the specific predetermined state in step (a) is dysplasia.
Claim 72
Dependent Claim
17 words
The method as recited in claim 57, wherein the specific predetermined state in step (a) is non-dysplasia.
Claim 73
Dependent Claim
17 words
The method as recited in claim 57, wherein the specific predetermined state in step (a) is precancer.
Claim 74
Dependent Claim
18 words
The method as recited in claim 57, wherein the specific predetermined state in step (a) is frank cancer.
Claim 75
Dependent Claim
16 words
The method as recited in claim 57, wherein the predetermined condition in step (d) is dysplasia.
Claim 76
Dependent Claim
16 words
The method as recited in claim 57, wherein the predetermined condition in step (d) is inflammation.
Claim 77
Dependent Claim
20 words
The method as recited in claim 57, wherein the frequency range of the Raman spectra is from ultraviolet to near-infrared.
Claim 78
Dependent Claim
20 words
The method as recited in claim 57, wherein the obtaining the first Raman spectra includes collecting said first spectra in-situ.
Claim 79
Dependent Claim
19 words
The method as recited in claim 74, wherein collecting said first spectra in-situ includes collecting with a sensing probe.
Claim 80
Independent Claim
117 words
A method for of detecting and diagnosing disease in epithelial cells, comprising the steps of: (a) obtaining and storing in storage means first resonance Raman spectra for at least one cell from a patient being tested that has been determined to have a specific predetermined state; (b) collecting cells from the patient being tested; (c) generating second Raman spectra from the cells collected at step (b); (d) comparing the first and second resonance Raman spectra for variations caused by a predetermined condition: and (e) diagnosing a change of levels of dysplasia based on the comparison at step (d) for determining a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 81
Independent Claim
117 words
A method for of detecting and diagnosing disease in epithelial cells, comprising the steps of: (a) obtaining and storing in storage means first resonance Raman spectra for at least one cell from a patient being tested that has been determined to have a specific predetermined state; (b) collecting cells from the patient being tested; (c) generating second Raman spectra from the cells collected at step (b); (d) comparing the first and second resonance Raman spectra for variations caused by a predetermined condition: and (e) diagnosing a change of levels of dysplasia based on the comparison at step (d) for determining a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 82
Dependent Claim
18 words
The method as recited in claim 76, wherein the collecting at step (b) is carried out by scraping.
Claim 83
Dependent Claim
19 words
The method as recited in claim 76, wherein the collecting at step (b) is carried out by surgical biopsy.
Claim 84
Dependent Claim
20 words
The method as recited in claim 76, wherein the collecting at step (b) is carried out by fine needle aspiration.
Claim 85
Dependent Claim
21 words
The method as recited in claim 76, wherein the collecting at step (b) is carried out by a collection of stool.
Claim 86
Dependent Claim
21 words
The method as recited in claim 76, wherein the collecting at step (b) is carried out by a collection of urine.
Claim 87
Dependent Claim
22 words
The method as recited in claim 76, wherein the method is used for detecting and diagnosing disease in epithelial cells in humans.
Claim 88
Dependent Claim
22 words
The method as recited in claim 76, wherein the method is used for detecting and diagnosing disease in epithelial cells in non-humans.
Claim 89
Dependent Claim
22 words
The method as recited in claim 83, wherein the method is used for detecting and diagnosing disease in epithelial cells in animals.
Claim 90
Dependent Claim
21 words
The method as recited in claim 76, wherein the method is used for detecting and diagnosing disease in epithelial cells in-vitro.
Claim 91
Dependent Claim
17 words
The method as recited in claim 76, wherein the specific predetermined state in step (a) is dysplasia.
Claim 92
Dependent Claim
17 words
The method as recited in claim 76, wherein the specific predetermined state in step (a) is non-dysplasia.
Claim 93
Dependent Claim
17 words
The method as recited in claim 76, wherein the specific predetermined state in step (a) is precancer.
Claim 94
Dependent Claim
18 words
The method as recited in claim 76, wherein the specific predetermined state in step (a) is frank cancer.
Claim 95
Dependent Claim
16 words
The method as recited in claim 76 wherein the predetermined condition in step (d) is dysplasia.
Claim 96
Dependent Claim
16 words
The method as recited in claim 76, wherein the predetermined condition in step (d) is inflammation.
Claim 97
Dependent Claim
20 words
The method as recited in claim 76, wherein the frequency range of the Raman spectra is from ultraviolet to near-infrared.
Claim 98
Dependent Claim
20 words
The method as recited in claim 76, wherein the obtaining the first Raman spectra includes collecting said first spectra in-situ.
Claim 99
Dependent Claim
19 words
The method as recited in claim 93, wherein collecting said first spectra in-situ includes collecting with a sensing probe.
Claim 100
Independent Claim
149 words
A method for detecting and diagnosing disease in epithelial cells for grading on a continuous scale of a predetermined range from non-dysplastic to fully dysplastic, comprising the steps of: (a) setting a first quantitative value along the continuous scale representative of a predetermined dysplastic condition for at least one cell of a patient being tested so that relative changes in a degree of dysplasia may be determined for said patient: (b) collecting cells from the patient being tested; (c) generating spectral information from the cells collected at step (b); (d) determining a second quantitative value along the continuous scale that is representative of spectral information generated at step (c); (e) comparing the first and second quantitative values on the continuous scale; and (f) determining based on the first and second quantitative values a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 101
Independent Claim
149 words
A method for detecting and diagnosing disease in epithelial cells for grading on a continuous scale of a predetermined range from non-dysplastic to fully dysplastic, comprising the steps of: (a) setting a first quantitative value along the continuous scale representative of a predetermined dysplastic condition for at least one cell of a patient being tested so that relative changes in a degree of dysplasia may be determined for said patient: (b) collecting cells from the patient being tested; (c) generating spectral information from the cells collected at step (b); (d) determining a second quantitative value along the continuous scale that is representative of spectral information generated at step (c); (e) comparing the first and second quantitative values on the continuous scale; and (f) determining based on the first and second quantitative values a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 102
Dependent Claim
22 words
The method as recited in claim 95, wherein the method is used for detecting and diagnosing disease in epithelial cells in humans.
Claim 103
Dependent Claim
22 words
The method as recited in claim 95, wherein the method is used for detecting and diagnosing disease in epithelial cells in non-humans.
Claim 104
Dependent Claim
22 words
The method as recited in claim 97, wherein the method is used for detecting and diagnosing disease in epithelial cells in animals.
Claim 105
Dependent Claim
21 words
The method as recited in claim 98, wherein the method is used for detecting and diagnosing disease in epithelial cells in-vitro.
Claim 106
Dependent Claim
18 words
The method as recited in claim 95, wherein the collecting at step (b) is carried out by scraping.
Claim 107
Dependent Claim
19 words
The method as recited in claim 95, wherein the collecting at step (b) is carried out by surgical biopsy.
Claim 108
Dependent Claim
20 words
The method as recited in claim 95, wherein the collecting at step (b) is carried out by fine needle aspiration.
Claim 109
Dependent Claim
21 words
The method as recited in claim 98, wherein the collecting at step (b) is carried out by a collection of stool.
Claim 110
Dependent Claim
21 words
The method as recited in claim 98, wherein the collecting at step (b) is carried out by a collection of urine.
Claim 111
Independent Claim
152 words
A method for detecting and diagnosing disease in epithelial cells for grading on a continuous scale of a predetermined range from non-dysplastic to fully dysplastic, comprising the steps of: (a) setting a first quantitative value along the continuous scale representative of a predetermined dysplastic condition for at least one cell in a cell culture being tested so that relative changes in a degree of dysplasia may be determined for said cell culture; (b) collecting cells from the cell culture being tested; (c) generating spectral information from the cell collected at step (b); (d) determining a second quantitative value along the continuous scale that is representative of spectral information generated at step (c); (e) comparing the first and second quantitative values on the continuous scale; and (f) determining based on the first and second quantitative values a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 112
Independent Claim
152 words
A method for detecting and diagnosing disease in epithelial cells for grading on a continuous scale of a predetermined range from non-dysplastic to fully dysplastic, comprising the steps of: (a) setting a first quantitative value along the continuous scale representative of a predetermined dysplastic condition for at least one cell in a cell culture being tested so that relative changes in a degree of dysplasia may be determined for said cell culture; (b) collecting cells from the cell culture being tested; (c) generating spectral information from the cell collected at step (b); (d) determining a second quantitative value along the continuous scale that is representative of spectral information generated at step (c); (e) comparing the first and second quantitative values on the continuous scale; and (f) determining based on the first and second quantitative values a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 113
Dependent Claim
22 words
The method as recited in claim 105, wherein the method is used for detecting and diagnosing disease in epithelial cells from humans.
Claim 114
Dependent Claim
22 words
The method as recited in claim 105, wherein the method is used for detecting and diagnosing disease in epithelial cells from non-humans.
Claim 115
Dependent Claim
22 words
The method as recited in claim 105, wherein the method is used for detecting and diagnosing disease in epithelial cells from animals.
Claim 116
Dependent Claim
21 words
The method as recited in claim 105, wherein the method is used for detecting and diagnosing disease in epithelial cells in-vitro.
Claim 117
Dependent Claim
18 words
The method as recited in claim 105, wherein the collecting at step (b) is carried out by scraping.
Claim 118
Dependent Claim
19 words
The method as recited in claim 105, wherein the collecting at step (b) is carried out by surgical biopsy.
Claim 119
Dependent Claim
20 words
The method as recited in claim 105, wherein the collecting at step (b) is carried out by fine needle aspiration.
Claim 120
Dependent Claim
21 words
The method as recited in claim 105, wherein the collecting at step (b) is carried out by a collection of stool.
Claim 121
Dependent Claim
21 words
The method as recited in claim 105, wherein the collecting at step (b) is carried out by a collection of urine.
Claim 122
Independent Claim
166 words
A method for detecting and diagnosing disease in cells, comprising the steps of: (a) obtaining first spectra in a predetermined frequency range for at least a first cell from a source; (b) comparing the first spectra with a set of spectral data indicative of a plurality of states of dysplasia and determining a state of dysplasia for the first cell; (c) collecting at least a second cell from the source; (d) generating second spectra in a predetermined frequency range for the second cell; (e) comparing the second spectra with the set of spectral data indicative of a plurality of states of dysplasia and determining a state of dysplasia for the second cell; (f) comparing the state of dysplasia for the first cell and the state of dysplasia for the second cell; and (g) diagnosing a change of levels of dysplasia based on the comparison at step (f) for determining a degree of progression or regression of dysplasia, or whether there have been no change in dysplasia.
Claim 123
Independent Claim
166 words
A method for detecting and diagnosing disease in cells, comprising the steps of: (a) obtaining first spectra in a predetermined frequency range for at least a first cell from a source; (b) comparing the first spectra with a set of spectral data indicative of a plurality of states of dysplasia and determining a state of dysplasia for the first cell; (c) collecting at least a second cell from the source; (d) generating second spectra in a predetermined frequency range for the second cell; (e) comparing the second spectra with the set of spectral data indicative of a plurality of states of dysplasia and determining a state of dysplasia for the second cell; (f) comparing the state of dysplasia for the first cell and the state of dysplasia for the second cell; and (g) diagnosing a change of levels of dysplasia based on the comparison at step (f) for determining a degree of progression or regression of dysplasia, or whether there have been no change in dysplasia.
Claim 124
Dependent Claim
19 words
The method as recited in claim 115, wherein the collecting step at step (c) is carried out by scraping.
Claim 125
Dependent Claim
20 words
The method as recited in claim 115, wherein the collecting step at step (c) is carried out by surgical biopsy.
Claim 126
Dependent Claim
21 words
The method as recited in claim 115, wherein the collecting step at step (c) is carried out by fine needle aspiration.
Claim 127
Dependent Claim
22 words
The method as recited in claim 115, wherein the collecting step at step (c) is carried out by a collection of stool.
Claim 128
Dependent Claim
22 words
The method as recited in claim 115, wherein the collecting step at step (c) is carried out by a collection of urine.
Claim 129
Dependent Claim
19 words
The method as recited in claim 115, wherein the method is used for detecting and diagnosing disease in humans.
Claim 130
Dependent Claim
19 words
The method as recited in claim 115, wherein the method is used for detecting and diagnosing disease in non-humans.
Claim 131
Dependent Claim
19 words
The method as recited in claim 115, wherein the method is used for detecting and diagnosing disease in animals.
Claim 132
Dependent Claim
21 words
The method as recited in claim 115, wherein obtaining first spectra in step (a) is carried out using infrared transmission techniques.
Claim 133
Dependent Claim
21 words
The method as recited in claim 115, wherein generating second spectra in step (d) is carried out using infrared transmission techniques.
Claim 134
Independent Claim
163 words
A method for detecting and diagnosing disease in cells, comprising the steps of: (a) obtaining and storing in storage means first spectra in at least one predetermined frequency range for at least a first cell from a source with the stored spectra being determined to have a specific state of dysplasia; (b) collecting at least a second cell from the source; (c) generating second spectra in at least one predetermined frequency range for the second cell collected at step (b); (d) comparing the second spectra with a set of spectral data indicative of a plurality of states of dysplasia and determining a state of dysplasia for the second cell; (e) comparing the state of dysplasia for the first cell with the state of dysplasia for the second cell; and (f) diagnosing a change of levels of dysplasia based on the comparison at step (e) for determining a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 135
Independent Claim
163 words
A method for detecting and diagnosing disease in cells, comprising the steps of: (a) obtaining and storing in storage means first spectra in at least one predetermined frequency range for at least a first cell from a source with the stored spectra being determined to have a specific state of dysplasia; (b) collecting at least a second cell from the source; (c) generating second spectra in at least one predetermined frequency range for the second cell collected at step (b); (d) comparing the second spectra with a set of spectral data indicative of a plurality of states of dysplasia and determining a state of dysplasia for the second cell; (e) comparing the state of dysplasia for the first cell with the state of dysplasia for the second cell; and (f) diagnosing a change of levels of dysplasia based on the comparison at step (e) for determining a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 136
Dependent Claim
19 words
The method as recited in claim 126, wherein the collecting step at step (b) is carried out by scraping.
Claim 137
Dependent Claim
20 words
The method as recited in claim 126, wherein the collecting step at step (b) is carried out by surgical biopsy.
Claim 138
Dependent Claim
21 words
The method as recited in claim 126, wherein the collecting step at step (b) is carried out by fine needle aspiration.
Claim 139
Dependent Claim
22 words
The method as recited in claim 126, wherein the collecting step at step (b) is carried out by a collection of stool.
Claim 140
Dependent Claim
22 words
The method as recited in claim 126, wherein the collecting step at step (b) is carried out by a collection of urine.
Claim 141
Dependent Claim
19 words
The method as recited in claim 126, wherein the method is used for detecting and diagnosing disease in humans.
Claim 142
Dependent Claim
19 words
The method as recited in claim 126, wherein the method is used for detecting and diagnosing disease in non-humans.
Claim 143
Dependent Claim
19 words
The method as recited in claim 126, wherein the method is used for detecting and diagnosing disease in animals.
Claim 144
Dependent Claim
21 words
The method as recited in claim 126, Wherein obtaining first spectra in step (a) is carried out using infrared transmission techniques.
Claim 145
Dependent Claim
21 words
The method as recited in claim 126, wherein generating second spectra in step (c) is carried out using infrared transmission techniques.
Claim 146
Independent Claim
159 words
A method for detecting and diagnosing disease in cells, comprising the steps of: (a) obtaining and storing in storage means first spectra in a near-infrared frequency range for at least a first cell from a source with the stored spectra being determined to have a specific state of dysplasia; (b) collecting at least a second cell from the source; (c) generating second spectra in a near-infrared frequency range for the second cell collected at step (b); (d) comparing the second spectra with a set of spectral data indicative of a plurality of states of dysplasia and determining a state of dysplasia for the second cell; (e) comparing the state of dysplasia for the first cell with the state of dysplasia for the second cell; and (f) diagnosing a change of levels of dysplasia based on the comparison at step (e) for determining a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 147
Independent Claim
159 words
A method for detecting and diagnosing disease in cells, comprising the steps of: (a) obtaining and storing in storage means first spectra in a near-infrared frequency range for at least a first cell from a source with the stored spectra being determined to have a specific state of dysplasia; (b) collecting at least a second cell from the source; (c) generating second spectra in a near-infrared frequency range for the second cell collected at step (b); (d) comparing the second spectra with a set of spectral data indicative of a plurality of states of dysplasia and determining a state of dysplasia for the second cell; (e) comparing the state of dysplasia for the first cell with the state of dysplasia for the second cell; and (f) diagnosing a change of levels of dysplasia based on the comparison at step (e) for determining a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 148
Dependent Claim
19 words
The method as recited in claim 137, wherein the collecting step at step (b) is carried out by scraping.
Claim 149
Dependent Claim
20 words
The method as recited in claim 137, wherein the collecting step at step (b) is carried out by surgical biopsy.
Claim 150
Dependent Claim
21 words
The method as recited in claim 137, wherein the collecting step at step (b) is carried out by fine needle aspiration.
Claim 151
Dependent Claim
22 words
The method as recited in claim 137, wherein the collecting step at step (b) is carried out by a collection of stool.
Claim 152
Dependent Claim
22 words
The method as recited in claim 137, wherein the collecting step at step (b) is carried out by a collection of urine.
Claim 153
Dependent Claim
19 words
The method as recited in claim 137, wherein the method is used for detecting and diagnosing disease in humans.
Claim 154
Dependent Claim
19 words
The method as recited in claim 137, wherein the method is used for detecting and diagnosing disease in non-humans.
Claim 155
Dependent Claim
19 words
The method as recited in claim 137, wherein the method is used for detecting and diagnosing disease in animals.
Claim 156
Dependent Claim
21 words
The method as recited in claim 137, wherein obtaining first spectra in step (a) is carried out using infrared transmission techniques.
Claim 157
Dependent Claim
21 words
The method as recited in claim 137, wherein generating second spectra in step (c) is carried out using infrared transmission techniques.
Claim 158
Independent Claim
151 words
A method for detecting and diagnosing disease in cells, comprising the steps of: (a) obtaining and storing in storage means first Raman spectra for at least a first cell from a source with the stored spectra being determined to have a specific state of dysplasia; (b) collecting at least a second cell from the source; (c) generating second Raman spectra for the second cell collected at step (b); (d) comparing the second spectra with a set of spectral data indicative of a plurality of states of dysplasia and determining a state of dysplasia for the second cell; (e) comparing the state of dysplasia for the first cell with the state of dysplasia for the second cell; and (f) diagnosing a change of levels of dysplasia based on the comparison at step (e) for determining a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 159
Independent Claim
151 words
A method for detecting and diagnosing disease in cells, comprising the steps of: (a) obtaining and storing in storage means first Raman spectra for at least a first cell from a source with the stored spectra being determined to have a specific state of dysplasia; (b) collecting at least a second cell from the source; (c) generating second Raman spectra for the second cell collected at step (b); (d) comparing the second spectra with a set of spectral data indicative of a plurality of states of dysplasia and determining a state of dysplasia for the second cell; (e) comparing the state of dysplasia for the first cell with the state of dysplasia for the second cell; and (f) diagnosing a change of levels of dysplasia based on the comparison at step (e) for determining a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 160
Dependent Claim
19 words
The method as recited in claim 148, wherein the collecting step at step (b) is carried out by scraping.
Claim 161
Dependent Claim
20 words
The method as recited in claim 148, wherein the collecting step at step (b) is carried out by surgical biopsy.
Claim 162
Dependent Claim
21 words
The method as recited in claim 148, wherein the collecting step at step (b) is carried out by fine needle aspiration.
Claim 163
Dependent Claim
22 words
The method as recited in claim 148, wherein the collecting step at step (b) is carried out by a collection of stool.
Claim 164
Dependent Claim
22 words
The method as recited in claim 148, wherein the collecting step at step (b) is carried out by a collection of urine.
Claim 165
Dependent Claim
19 words
The method as recited in claim 148, wherein the method is used for detecting and diagnosing disease in humans.
Claim 166
Dependent Claim
19 words
The method as recited in claim 148, wherein the method is used for detecting and diagnosing disease in non-humans.
Claim 167
Dependent Claim
19 words
The method as recited in claim 148, wherein the method is used for detecting and diagnosing disease in animals.
Claim 168
Dependent Claim
20 words
The method as recited in claim 148, wherein obtaining first spectra in step (a) is carried out using Raman techniques.
Claim 169
Dependent Claim
20 words
The method as recited in claim 148, wherein generating second spectra in step (c) is carried out using Raman techniques.
Claim 170
Independent Claim
153 words
A method for detecting and diagnosing disease in cells, comprising the steps of: (a) obtaining and storing in storage means first resonance Raman spectra for at least a first cell from a source with the stored spectra being determined to have a specific state of dysplasia; (b) collecting at least a second cell from the source; (c) generating second resonance Raman spectra for the second cell collected at step (b); (d) comparing the second spectra with a set of spectra data indicative of a plurality of states of dysplasia and determining a state of dysplasia for the second cell; (e) comparing the state of dysplasia for the first cell with the state of dysplasia for the second cell; and (f) diagnosing a change of levels of dysplasia based on the comparison at step (e) for determining a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 171
Independent Claim
153 words
A method for detecting and diagnosing disease in cells, comprising the steps of: (a) obtaining and storing in storage means first resonance Raman spectra for at least a first cell from a source with the stored spectra being determined to have a specific state of dysplasia; (b) collecting at least a second cell from the source; (c) generating second resonance Raman spectra for the second cell collected at step (b); (d) comparing the second spectra with a set of spectra data indicative of a plurality of states of dysplasia and determining a state of dysplasia for the second cell; (e) comparing the state of dysplasia for the first cell with the state of dysplasia for the second cell; and (f) diagnosing a change of levels of dysplasia based on the comparison at step (e) for determining a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 172
Dependent Claim
19 words
The method as recited in claim 159, wherein the collecting step at step (b) is carried out by scraping.
Claim 173
Dependent Claim
20 words
The method as recited in claim 159, wherein the collecting step at step (b) is carried out by surgical biopsy.
Claim 174
Dependent Claim
21 words
The method as recited in claim 159, wherein the collecting step at step (b) is carried out by fine needle aspiration.
Claim 175
Dependent Claim
22 words
The method as recited in claim 159, wherein the collecting step at step (b) is carried out by a collection of stool.
Claim 176
Dependent Claim
22 words
The method as recited in claim 159, wherein the collecting step at step (b) is carried out by a collection of urine.
Claim 177
Dependent Claim
19 words
The method as recited in claim 159, wherein the method is used for detecting and diagnosing disease in humans.
Claim 178
Dependent Claim
19 words
The method as recited in claim 159, wherein the method is used for detecting and diagnosing disease in non-humans.
Claim 179
Dependent Claim
19 words
The method as recited in claim 159, wherein the method is used for detecting and diagnosing disease in animals.
Claim 180
Dependent Claim
21 words
The method as recited in claim 159, wherein obtaining first spectra in step (a) is carried out using resonance Raman techniques.
Claim 181
Dependent Claim
21 words
The method as recited in claim 159, wherein generating second spectra in step (c) is carried out using resonance Raman techniques.
Claim 182
Independent Claim
144 words
A method for detecting and diagnosing disease in cells for grading on a continuous scale of a predetermined range from non-dysplastic to fully dysplastic, comprising the steps of: (a) setting a first quantitative value along the continuous scale representative of a predetermined dysplastic condition for at least one cell of a source so that relative changes in a degree of dysplasia may be determined for the source; (b) collecting cells from the source; (c) generating spectral information from the cells collected at step (b); (d) determining a second quantitative value along the continuous scale that is representative of spectral information generated at step (c); (e) comparing the first and second quantitative values on the continuous scale; and (f) determining based on the first and second quantitative values a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 183
Independent Claim
144 words
A method for detecting and diagnosing disease in cells for grading on a continuous scale of a predetermined range from non-dysplastic to fully dysplastic, comprising the steps of: (a) setting a first quantitative value along the continuous scale representative of a predetermined dysplastic condition for at least one cell of a source so that relative changes in a degree of dysplasia may be determined for the source; (b) collecting cells from the source; (c) generating spectral information from the cells collected at step (b); (d) determining a second quantitative value along the continuous scale that is representative of spectral information generated at step (c); (e) comparing the first and second quantitative values on the continuous scale; and (f) determining based on the first and second quantitative values a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 184
Dependent Claim
19 words
The method as recited in claim 170, wherein the collecting step at step (b) is carried out by scraping.
Claim 185
Dependent Claim
20 words
The method as recited in claim 170, wherein the collecting step at step (b) is carried out by surgical biopsy.
Claim 186
Dependent Claim
21 words
The method as recited in claim 170, wherein the collecting step at step (b) is carried out by fine needle aspiration.
Claim 187
Dependent Claim
22 words
The method as recited in claim 170, wherein the collecting step at step (b) is carried out by a collection of stool.
Claim 188
Dependent Claim
22 words
The method as recited in claim 170, wherein the collecting step at step (b) is carried out by a collection of urine.
Claim 189
Dependent Claim
19 words
The method as recited in claim 170, wherein the method is used for detecting and diagnosing disease in humans.
Claim 190
Dependent Claim
19 words
The method as recited in claim 170, wherein the method is used for detecting and diagnosing disease in non-humans.
Claim 191
Dependent Claim
19 words
The method as recited in claim 170, wherein the method is used for detecting and diagnosing disease in animals.
Claim 192
Dependent Claim
20 words
The method as recited in claim 170, generating spectral information in step (c) is carried out using infrared transmission techniques.
Claim 193
Independent Claim
145 words
A method for detecting and diagnosing disease in cells for grading on a continuous scale of a predetermined range from non-dysplastic to fully dysplastic, comprising the steps of: (a) setting a first quantitative value along the continuous scale representative of a predetermined dysplastic condition for at least one cell in a cell culture so that relative changes in a degree of dysplasia may be determined for the source; (b) collecting cells from the source; (c) generating spectral information from the cells collected at step (b); (d) determining a second quantitative value along the continuous scale that is representative of spectral information generated at step (c); (e) comparing the first and second quantitative values on the continuous scale; and (f) determining based on the first and second quantitative values a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 194
Independent Claim
145 words
A method for detecting and diagnosing disease in cells for grading on a continuous scale of a predetermined range from non-dysplastic to fully dysplastic, comprising the steps of: (a) setting a first quantitative value along the continuous scale representative of a predetermined dysplastic condition for at least one cell in a cell culture so that relative changes in a degree of dysplasia may be determined for the source; (b) collecting cells from the source; (c) generating spectral information from the cells collected at step (b); (d) determining a second quantitative value along the continuous scale that is representative of spectral information generated at step (c); (e) comparing the first and second quantitative values on the continuous scale; and (f) determining based on the first and second quantitative values a degree of progression or regression of dysplasia, or whether there has been no change in dysplasia.
Claim 195
Dependent Claim
19 words
The method as recited in claim 180, wherein the collecting step at step (b) is carried out by scraping.
Claim 196
Dependent Claim
20 words
The method as recited in claim 180, wherein the collecting step at step (b) is carried out by surgical biopsy.
Claim 197
Dependent Claim
21 words
The method as recited in claim 180, wherein the collecting step at step (b) is carried out by fine needle aspiration.
Claim 198
Dependent Claim
22 words
The method as recited in claim 180, wherein the collecting step at step (b) is carried out by a collection of stool.
Claim 199
Dependent Claim
22 words
The method as recited in claim 180, wherein the collecting step at step (b) is carried out by a collection of urine.
Claim 200
Dependent Claim
19 words
The method as recited in claim 180, wherein the method is used for detecting and diagnosing disease in humans.
Claim 201
Dependent Claim
19 words
The method as recited in claim 180, wherein the method is used for detecting and diagnosing disease in non-humans.
Claim 202
Dependent Claim
19 words
The method as recited in claim 180, wherein the method is used for detecting and diagnosing disease in animals.
Claim 203
Dependent Claim
20 words
The method as recited in claim 180, generating spectral information in step (c) is carried out using infrared transmission techniques.
References Cited (Backward Citations)
US3449571A
Cited
CA991438A
Cited
CA1036385A
Cited
CA1019975A
Cited
US4017192A
Cited
CA1149631A
Cited
US4515165A
Cited
US4778593A
Cited
US4855243A
Cited
US4735504A
Cited
US5073498A
Cited
US4832483A
Cited
US4905169A
Cited
US5408307A
Cited
US4975581A
Cited
CA2019865A1
Cited
US5107422A
Cited
US5072382A
Cited
CA2008831A1
Cited
US5038039A
Cited
CA2035603A1
Cited
US5168162A
Cited
US5261410A
Cited
CA2104960A1
Cited
WO1992015008A1
Cited
CA2050761A1
Cited
WO1993003672A1
Cited
US5281825A
Cited
US5596992A
Cited
WO1995011624A2
Cited
WO1995026502A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 2001-03-30 | 2001-09-29 | 2002-03-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-03-30 | 2005-09-29 | 2006-03-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-03-30 | 2009-09-29 | 2010-03-31 | $7700.00 | Window Closed / Lapsed |