US4832483A
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Method of using resonance raman spectroscopy for detection of malignancy disease
Assignee: Tufts Medical Center Inc
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Filing: 1987-09-03
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Granted: 1989-05-23
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Expires: 2007-09-03
Abstract
Abstract A method for determining the presence of malignancy disease in a subject person includes obtaining a test sample of blood plasma from the person, measuring intensities of Raman scattering from the plasma at frequencies in the frequency shift range between 1000 cm -1 and 1600 cm -1 , and comparing the intensities to intensities of Raman scattering, at comparable frequencies, from normal human plasma.
Inventors
- 👤 Surendra P. Verma
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:17
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/62; G01N21/63; G01N21/65; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/575
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
76 words
A method for determining the presence of malignancy disease in a subject person, comprising obtaining a test sample of blood plasma from the person, and measuring intensities of Raman scattering from said plasma at frequencies in the frequency shift range between 1000 cm -1 and 1600 cm -1 , a substantial difference between said intensities and intensities of Raman scattering, at comparable frequencies, from normal human plasma indicating a presence of malignancy disease in the person.
Claim 2
Independent Claim
76 words
A method for determining the presence of malignancy disease in a subject person, comprising obtaining a test sample of blood plasma from the person, and measuring intensities of Raman scattering from said plasma at frequencies in the frequency shift range between 1000 cm -1 and 1600 cm -1 , a substantial difference between said intensities and intensities of Raman scattering, at comparable frequencies, from normal human plasma indicating a presence of malignancy disease in the person.
Claim 3
Dependent Claim
38 words
The method of claim 1 wherein said method involves recording a plurality of said intensities of said test sample and comparing said plurality of recorded intensities to a plurality of intensities, at comparable frequencies, of said normal plasma.
Claim 4
Independent Claim
129 words
A method for detecting the presence of malignancy disease in a subject person, comprising obtaining a test sample of blood plasma from the person, recording intensities of Raman scattering from said plasma at frequencies in the frequency shift range between 1000 cm -1 and 1600 cm -1 , and determining from said recording a frequency at which the pseudo-Raman band reaches a maximum in said frequency shift range, a said pseudo-Raman band maximum at a frequency in the frequency shift range between 1000 cm -1 and 1350 cm -1 indicating a presence of malignancy disease in the person, and a said pseudo-Raman band maximum at a frequency in the frequency shift range between 1350 cm -1 and 1600 cm -1 indicating an absence of malignancy disease in the person.
Claim 5
Independent Claim
129 words
A method for detecting the presence of malignancy disease in a subject person, comprising obtaining a test sample of blood plasma from the person, recording intensities of Raman scattering from said plasma at frequencies in the frequency shift range between 1000 cm -1 and 1600 cm -1 , and determining from said recording a frequency at which the pseudo-Raman band reaches a maximum in said frequency shift range, a said pseudo-Raman band maximum at a frequency in the frequency shift range between 1000 cm -1 and 1350 cm -1 indicating a presence of malignancy disease in the person, and a said pseudo-Raman band maximum at a frequency in the frequency shift range between 1350 cm -1 and 1600 cm -1 indicating an absence of malignancy disease in the person.
Claim 6
Dependent Claim
66 words
The method of claim 3, a said pseudo-Raman band maximum at a frequency in the frequency shift range between 1160 cm -1 and 1250 cm -1 indicating a presence of malignancy disease in the person, and a said pseudo-Raman band maximum at a frequency in the frequency shift range between 1450 cm -1 and 1530 cm -1 indicating an absence of malignancy disease in the person.
Claim 7
Independent Claim
134 words
A method for detecting the presence of malignancy disease in a subject person, comprising obtaining a test sample of blood plasma from the person, recording intensities of Raman scattering from said plasma at frequencies in the frequency shift range between 1000 cm -1 and 1600 cm -1 , determining from said recording the height, I 1530 , of the 1530 cm -1 peak at its maximum intensity, and determining from said recording the height of the 1160 cm -1 peak at its maximum intensity, I 1160 , a said ratio I 1530 /I 1160 in the range between 1.30 and 1.59 indicating a presence of malignancy disease in the person, and a said ratio I 1530 /I 1160 in the range between 1.00 and 1.20 indicating an absence of malignancy disease in the person.
Claim 8
Independent Claim
134 words
A method for detecting the presence of malignancy disease in a subject person, comprising obtaining a test sample of blood plasma from the person, recording intensities of Raman scattering from said plasma at frequencies in the frequency shift range between 1000 cm -1 and 1600 cm -1 , determining from said recording the height, I 1530 , of the 1530 cm -1 peak at its maximum intensity, and determining from said recording the height of the 1160 cm -1 peak at its maximum intensity, I 1160 , a said ratio I 1530 /I 1160 in the range between 1.30 and 1.59 indicating a presence of malignancy disease in the person, and a said ratio I 1530 /I 1160 in the range between 1.00 and 1.20 indicating an absence of malignancy disease in the person.
Claim 9
Independent Claim
76 words
A method for detecting the presence of malignancy disease in a subject person, comprising obtaining a test sample of blood plasma from the person, and recording intensities of Raman scattering from said plasma at frequencies in the frequency shift range between 1400 cm -1 and 1600 cm -1 , a substantial difference between said intensities and intensities of Raman scattering, at comparable frequencies, from normal human plasma indicating a presence of malignancy disease in the person.
Claim 10
Independent Claim
76 words
A method for detecting the presence of malignancy disease in a subject person, comprising obtaining a test sample of blood plasma from the person, and recording intensities of Raman scattering from said plasma at frequencies in the frequency shift range between 1400 cm -1 and 1600 cm -1 , a substantial difference between said intensities and intensities of Raman scattering, at comparable frequencies, from normal human plasma indicating a presence of malignancy disease in the person.
Claim 11
Independent Claim
121 words
A method for detecting the presence of malignancy disease in a subject person, comprising obtaining a test sample of blood plasma from the person, recording intensities of Raman scattering from said plasma at frequencies in the frequency shift range between 1500 cm -1 and 1560 cm -1 , determining from said recording the shape of that portion of the 1530 cm -1 band between 1500 cm -1 and 1560 cm -1 , and measuring the half-height band width of said 1530 cm -1 band, a said half-height band width of 16.5±2.6 cm -1 indicating a presence of malignancy disease in the person, and a said half-height band width of 22±2.5 cm -1 indicating an absence of malignancy disease in the person.
Claim 12
Independent Claim
121 words
A method for detecting the presence of malignancy disease in a subject person, comprising obtaining a test sample of blood plasma from the person, recording intensities of Raman scattering from said plasma at frequencies in the frequency shift range between 1500 cm -1 and 1560 cm -1 , determining from said recording the shape of that portion of the 1530 cm -1 band between 1500 cm -1 and 1560 cm -1 , and measuring the half-height band width of said 1530 cm -1 band, a said half-height band width of 16.5±2.6 cm -1 indicating a presence of malignancy disease in the person, and a said half-height band width of 22±2.5 cm -1 indicating an absence of malignancy disease in the person.
Claim 13
Dependent Claim
30 words
The method of claim 3, 5, 6, or 7 wherein said intensities of Raman scattering are recorded at regular intervals over at least a portion of said frequency shift range.
Claim 14
Dependent Claim
16 words
The method of claim 8 wherein said intervals are no greater than 100 cm -1 .
Claim 15
Dependent Claim
16 words
The method of claim 8 wherein said intervals are no greater than 50 cm -1 .
Claim 16
Dependent Claim
29 words
The method of claim 8 wherein said intervals are sufficiently small that together said recorded intensities comprise a substantially continuous spectrograph over said portion of said frequency shift range.
References Cited (Backward Citations)
No citation records indexed.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1992-05-22 | 1992-11-21 | 1993-05-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-05-22 | 1996-11-21 | 1997-05-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-05-22 | 2000-11-21 | 2001-05-23 | $7700.00 | Window Closed / Lapsed |