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US5689045A
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Transgenic pathogen-resistant plant
Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften eV
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Filing: 1995-06-01
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Granted: 1997-11-18
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Expires: 2015-06-01
Abstract
Abstract Transgenic pathogen-resistant organism whose genome contains at least two different genes under the control of active promoters with pathogen-inhibiting action. This organism is distinguished by a synergistic pathogen-inhibiting action. This action is evident particularly when the genes code for the gene products chitinase (ChiS, ChiG), glucanase (GluG), protein synthesis inhibitor (PSI) and antifungal protein (AFP).
Inventors
- 👤 Jurgen Logemann
- 👤 Guido Jach
- 👤 Birgit Gornhardt
- 👤 John Mundy
- 👤 Jeff Schell
- 👤 Peter Eckes
- 👤 Ilan Chet
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 05:17
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/37; C07K14/415; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8241; C12N15/8261; C12N15/8271; C12N15/8279; C12N15/8282; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2434; C12N9/2442; C12Y; C12Y302/00; C12Y302/01; C12Y302/01014; Y; Y10; Y10S; Y10S47/00; Y10S47/01
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
123 words
A transgenic plant comprising (a) at least one gene, operably linked to a plant-functional promoter, said gene being selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4, and an AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1, and (b) a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8, operably linked to a plant-functional promoter, wherein the plant is resistant to fungal attack.
Claim 2
Dependent Claim
123 words
A transgenic plant comprising (a) at least one gene, operably linked to a plant-functional promoter, said gene being selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4, and an AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1, and (b) a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8, operably linked to a plant-functional promoter, wherein the plant is resistant to fungal attack.
Claim 3
Dependent Claim
123 words
A transgenic plant comprising (a) at least one gene operably linked to a plant-functional promoter, said gene being selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4, and a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8, and (b) a AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1, operably linked to a plant-functional promoter, wherein the plant is resistant to fungal attack.
Claim 4
Dependent Claim
123 words
A transgenic plant comprising (a) at least one gene operably linked to a plant-functional promoter, said gene being selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4, and a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8, and (b) a AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1, operably linked to a plant-functional promoter, wherein the plant is resistant to fungal attack.
Claim 5
Dependent Claim
23 words
A transgenic fungus-resistant plant according to claim 1 or 2 wherein the plant is a tobacco, potato, strawberry, corn, rape or tomato plant.
Claim 6
Dependent Claim
103 words
A purified and isolated DNA molecule comprising (a) at least one gene selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4, and an AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1, and (b) a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8.
Claim 7
Dependent Claim
103 words
A purified and isolated DNA molecule comprising (a) at least one gene selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4, and an AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1, and (b) a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8.
Claim 8
Dependent Claim
103 words
A purified and isolated DNA molecule comprising (a) at least one gene selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4, and a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8, and (b) an AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1.
Claim 9
Dependent Claim
103 words
A purified and isolated DNA molecule comprising (a) at least one gene selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4, and a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8, and (b) an AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1.
Claim 10
Dependent Claim
84 words
A process for the generation of a fungus-resistant plant according to claim 1 or 2, wherein a plant is transformed with at least one gene with fungus-inhibiting action, and the fungus-resistant plant is obtained by a method selected from the group consisting of (a) crossing the plant with another plant which contains at least one other gene, said gene having fungus-inhibiting action, and subsequently selecting the fungus-resistant plant, and (b) transforming the plant with at least one other gene, said gene having fungus-inhibiting action.
Claim 11
Dependent Claim
104 words
A process according to claim 6 which employs a DNA transfer vector comprising a gene selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4, an AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1, and a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8.
Claim 12
Dependent Claim
131 words
A process for the generation of a transgenic fungus-resistant plant comprising employing a DNA-vector comprising (a) at least one gene operably linked to a plant-functional promoter, said gene being selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4 and a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8, and (b) an AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1, operably linked to a plant-functional promoter, for transformation of a plant.
Claim 13
Dependent Claim
131 words
A process for the generation of a transgenic fungus-resistant plant comprising employing a DNA-vector comprising (a) at least one gene operably linked to a plant-functional promoter, said gene being selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4 and a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8, and (b) an AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1, operably linked to a plant-functional promoter, for transformation of a plant.
Claim 14
Dependent Claim
131 words
A process for the generation of transgenic fungus-resistant plant, comprising employing a DNA vector comprising (a) at least one gene operably linked to a plant-functional promoter, said gene being selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4 and an AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1, and (b) a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8, operably linked to a plant-functional promoter, for transformation of a plant.
Claim 15
Dependent Claim
131 words
A process for the generation of transgenic fungus-resistant plant, comprising employing a DNA vector comprising (a) at least one gene operably linked to a plant-functional promoter, said gene being selected from the group consisting of a ChiG gene from barley having a sequence as set forth in SEQUENCE ID NO. 9, a GluG gene from barley having a sequence as set forth in SEQUENCE ID NO. 11, a PSI gene from barley having a sequence as set forth in SEQUENCE ID NO. 4 and an AFP gene from Aspergillus giganteus having a sequence as set forth in SEQUENCE ID NO. 1, and (b) a ChiS gene from Serratia marcescens having a sequence as set forth in SEQUENCE ID NO. 8, operably linked to a plant-functional promoter, for transformation of a plant.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-11-17 | 2001-05-19 | 2001-11-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-11-17 | 2005-05-19 | 2005-11-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-11-17 | 2009-05-19 | 2009-11-18 | $7700.00 | Window Closed / Lapsed |