US4940840A
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Novel chitinase-producing bacteria and plants
Assignee: DNA Plant Technology Corp
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Filing: 1986-07-18
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Granted: 1990-07-10
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Expires: 2006-07-18
Abstract
Abstract Novel bacteria strains are decribed which are created by the introduction of DNA encoding for the production of chitinase, an enzyme capable of degrading chitin present in fungi and nematodes. The strains have utility in producing chitinase for the purpose of inhibiting plant pathogens. Novel pathogen-resistant plants are also described which are created by introduction of DNA encoding for the production of chitinase.
Inventors
- 👤 Trevor V. Suslow
- 👤 Jonathan D. G. Jones
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 05:23
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8241; C12N15/8242; C12N15/8243; C12N15/8245; C12N15/8246; A; A01; A01N; A01N63/00; A01N63/20; C12N15/8261; C12N15/8271; C12N15/8273; C12N15/8279; C12N15/8282; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2434; C12N9/2442; C12Y; C12Y302/00; C12Y302/01; C12Y302/01014; C12R; C12R2001/00; C12R2001/01; C12R2001/38; C12R2001/40
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
36 words
A method of inhibiting chitinous fungal pathogens comprising introducing into a dicotyledonous plant a DNA sequence encoding for chitinase activity to create a transformed plant under conditions whereby the transformed plant expresses chitinase in active form.
Claim 2
Independent Claim
36 words
A method of inhibiting chitinous fungal pathogens comprising introducing into a dicotyledonous plant a DNA sequence encoding for chitinase activity to create a transformed plant under conditions whereby the transformed plant expresses chitinase in active form.
Claim 3
Dependent Claim
15 words
The method of claim 1 wherein said DNA sequence is isolated from a bacterial source.
Claim 4
Dependent Claim
22 words
The method of claim 1 wherein said DNA sequence is substantially homologous to chitinase-encoding DNA contained in ATCC #39637 or ATCC #67152.
Claim 5
Dependent Claim
15 words
The method of claim 1 wherein said DNA sequence is fused to a plant promoter.
Claim 6
Dependent Claim
13 words
The method of claim 1 wherein said DNA sequence is introduced using Agrobacterium.
Claim 7
Dependent Claim
24 words
The method of claim 1 wherein the transformed plant expresses chitinase in biologically active form as measurable by an assay using Tobacco Brown Spot.
Claim 8
Dependent Claim
43 words
A transformed dicotyledonous plant resistant to chitinous fungal pathogens, said transformed plant containing a DNA sequence encoding for chitinase activity and said DNA sequence having been introduced into the plant by transformation under conditions whereby the transformed plant expresses chitinase in active form.
Claim 9
Dependent Claim
43 words
A transformed dicotyledonous plant resistant to chitinous fungal pathogens, said transformed plant containing a DNA sequence encoding for chitinase activity and said DNA sequence having been introduced into the plant by transformation under conditions whereby the transformed plant expresses chitinase in active form.
Claim 10
Dependent Claim
15 words
The plant of claim 7 wherein said DNA sequence is isolated from a bacterial source.
Claim 11
Dependent Claim
22 words
The plant of claim 7 wherein said DNA sequence is substantially homologous to chitinase-encoding DNA contained in ATCC #39637 or ATCC #67152.
Claim 12
Dependent Claim
15 words
The plant of claim 7 wherein said DNA sequence is fused to a plant promoter.
Claim 13
Dependent Claim
13 words
The plant of claim 7 wherein said DNA sequence is introduced using Agrobacterium.
Claim 14
Dependent Claim
24 words
The plant of claim 7 wherein the transformed plant expresses chitinase in biologically active form as measurable by an assay using Tobacco Brown Spot.
References Cited (Backward Citations)
No citation records indexed.
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