Patent US5688233A successfully refreshed from Google Patents.
US5688233A
Expired / Lapsed
US
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Electronincorporation enhanced transdermal delivery of molecules
Assignee: Genetronics Inc
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Filing: 1995-11-02
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Granted: 1997-11-18
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Expires: 2015-11-02
Abstract
Abstract A method of transdermal molecular delivery comprising molecules to be delivered mixed with particles, contacting a selected area of a skin surface with the particles and molecules, applying a pulsed electric field of sufficient amplitude and duration to induce dielectric breakdown of the stratum corneum, and applying a pressure to the molecules to force transport of the molecules through the pores in the stratum corneum into the underlying skin.
Inventors
- 👤 Gunter A. Hofmann
- 👤 Lei Zhang
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 16:12
CPC Classifications
A; A61; A61N; A61N1/00; A61N1/18; A61N1/20; A61N1/30; A61N1/303; A61N1/02; A61N1/04; A61N1/0404; A61N1/0408; A61N1/0412; A61N1/0416; A61N1/0424; A61N1/32; A61N1/325; A61M; A61M37/00; A61M2037/0007; A61N1/0428; A61N1/0432; A61N1/044; A61N1/306
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
76 words
A method of transdermal molecular delivery comprising the steps of: providing molecules to be delivered through a stratum corneum; providing a solution containing particles and said molecules; contacting a selected surface area of the stratum corneum with said solution; applying a pulsed electric field of sufficient amplitude to induce pores in the stratum corneum; and applying a force to said molecules sufficient to induce transport of said molecules through the induced pores in the stratum corneum.
Claim 2
Independent Claim
76 words
A method of transdermal molecular delivery comprising the steps of: providing molecules to be delivered through a stratum corneum; providing a solution containing particles and said molecules; contacting a selected surface area of the stratum corneum with said solution; applying a pulsed electric field of sufficient amplitude to induce pores in the stratum corneum; and applying a force to said molecules sufficient to induce transport of said molecules through the induced pores in the stratum corneum.
Claim 3
Dependent Claim
40 words
A method according to claim 1 wherein the step of applying said electric field includes applying said field with a strength of from about 10 to about 60 kV/cm with a pulse length of from 10 μsec to 100 msec.
Claim 4
Dependent Claim
21 words
A method according to claim 2 wherein said force is applied to said molecules within one minute of the electric field.
Claim 5
Dependent Claim
12 words
A method according to claim 4 wherein said force is a vacuum.
Claim 6
Dependent Claim
17 words
A method according to claim 3 wherein said force is applied by pressurized fluid carrying said molecules.
Claim 7
Dependent Claim
14 words
A method according to claim 1 wherein said force is applied by an electrode.
Claim 8
Dependent Claim
17 words
A method according to claim 1 wherein said force is applied by the weight of an electrode.
Claim 9
Dependent Claim
31 words
A method according to claim 1 wherein said electrical field is applied by a pair of spaced electrodes mounted on a caliper apparatus, and said force is applied by said calipers.
Claim 10
Dependent Claim
15 words
A method according to claim 1 wherein said force is applied by an electric field.
Claim 11
Independent Claim
74 words
A method of transdermal molecular delivery comprising the steps of: contacting a selected surface area of a stratum corneum with a quantity of molecules to be delivered through the stratum corneum; applying a pulsed electric field of sufficient amplitude to induce pores in the stratum corneum; and applying a force to said molecules sufficient to induce transport of said molecules through the induced pores in the stratum corneum wherein said force is a vacuum.
Claim 12
Independent Claim
74 words
A method of transdermal molecular delivery comprising the steps of: contacting a selected surface area of a stratum corneum with a quantity of molecules to be delivered through the stratum corneum; applying a pulsed electric field of sufficient amplitude to induce pores in the stratum corneum; and applying a force to said molecules sufficient to induce transport of said molecules through the induced pores in the stratum corneum wherein said force is a vacuum.
Claim 13
Dependent Claim
49 words
A method according to claim 10 wherein said electric field is applied by means of a plurality of closely spaced electrodes applied to the stratum corneum and is applied as pulses of from 10 to several hundred volts with a pulse length of between 10 μsec to 100 msec.
Claim 14
Dependent Claim
25 words
A method according to claim 11 wherein said molecules are in a solution which includes particles, and said particles are electroincorporated along with said solution.
Claim 15
Dependent Claim
24 words
A method according to claim 12 wherein said particles are selected from the group consisting of inert particles, biodegradable particles and particles containing molecules.
Claim 16
Dependent Claim
14 words
A method according to claim 12 wherein said inert particles are particles of gold.
Claim 17
Dependent Claim
15 words
A method according to claim 12 wherein some of said particles are vesicles containing molecules.
Claim 18
Independent Claim
123 words
A method of transdermal molecular delivery comprising the steps of: contacting a selected surface area of the stratum corneum with a quantity of molecules to be delivered through a stratum corneum; applying a pulsed electric field of sufficient amplitude to induce pores in the stratum corneum, said electric field is applied by means of a plurality of closely spaced electrodes applied to the surface of the stratum corneum and is applied as pulses of from 10 to several hundred volts with a pulse length of between 10 μsec to 100 msec; and applying a force to said molecules sufficient to induce transport of said fluid through the pores in the stratum corneum wherein said pressure is applied by pressurized fluid carrying said molecules.
Claim 19
Independent Claim
123 words
A method of transdermal molecular delivery comprising the steps of: contacting a selected surface area of the stratum corneum with a quantity of molecules to be delivered through a stratum corneum; applying a pulsed electric field of sufficient amplitude to induce pores in the stratum corneum, said electric field is applied by means of a plurality of closely spaced electrodes applied to the surface of the stratum corneum and is applied as pulses of from 10 to several hundred volts with a pulse length of between 10 μsec to 100 msec; and applying a force to said molecules sufficient to induce transport of said fluid through the pores in the stratum corneum wherein said pressure is applied by pressurized fluid carrying said molecules.
Claim 20
Dependent Claim
15 words
A method according to claim 16 wherein said quantity of molecules includes particles of gold.
Claim 21
Dependent Claim
22 words
A method according to claim 16 wherein said quantity of molecules includes particles and some of said particles are vesicles containing molecules.
Claim 22
Independent Claim
78 words
A method of transdermal molecular delivery comprising the steps of: incorporating molecules to be delivered in a carrier containing particles; contacting a selected area of a stratum corneum with said carrier of the molecules; applying a pulsed electric field of sufficient amplitude and duration to induce pores by dielectric breakdown of the stratum corneum; and applying a pressure to the molecules to force transport of the molecules through the induced pores in the stratum corneum into underlying tissue.
Claim 23
Independent Claim
78 words
A method of transdermal molecular delivery comprising the steps of: incorporating molecules to be delivered in a carrier containing particles; contacting a selected area of a stratum corneum with said carrier of the molecules; applying a pulsed electric field of sufficient amplitude and duration to induce pores by dielectric breakdown of the stratum corneum; and applying a pressure to the molecules to force transport of the molecules through the induced pores in the stratum corneum into underlying tissue.
Claim 24
Dependent Claim
41 words
A method according to claim 19 wherein said the step of applying said electric field includes applying said field with a strength of from about 10 to about 60 kV/cm with a pulse length of from 10 μsec to 100 msec.
Claim 25
Dependent Claim
17 words
A method according to claim 20 wherein said pressure is applied by pressurized fluid carrying said molecules.
Claim 26
Dependent Claim
52 words
A method according to claim 20 wherein said electric field is applied by means of a plurality of closely spaced electrodes applied to the surface of the stratum corneum and is applied as pulses of from 10 to several hundred volts with a pulse length of between 10 μsec to 100 msec.
Claim 27
Dependent Claim
21 words
A method according to claim 20 wherein said solution includes inert particles, and said particles are electroincorporated along with said solution.
Claim 28
Dependent Claim
14 words
A method according to claim 23 wherein said inert particles are particles of gold.
Claim 29
Dependent Claim
15 words
A method according to claim 24 wherein some of said particles are vesicles containing molecules.
Claim 30
Dependent Claim
22 words
A method according to claim 19 wherein said step of applying said pressure is carried out by applying pressure by an electrode.
Claim 31
Dependent Claim
23 words
A method according to claim 26 wherein said pressure is applied to said carrier within one minute after application of the electric field.
Claim 32
Dependent Claim
15 words
A method according to claim 16 wherein said force is applied by an electric field.
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