US4955378A
Expired / Lapsed
US
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Apparatus and methods for performing electrofusion at specific anatomical sites
Assignee: University of South Florida
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Filing: 1989-01-17
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Granted: 1990-09-11
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Expires: 2009-01-17
Abstract
Abstract Electrofusion of biological particles to specific areas of tissue is accomplished in vivo through the use of electrode members that conform to the configuration and dimension of the tissue at the electrofusion site. The electrode members are positioned in close physical proximity to one another so that when an electrical potential difference is established between them, current flow is limited to the area of tissue between the electrodes so that tissue remote from the selected electrofusion site is substantially unaffected by such current flow. A general apparatus and method is supplemented with two illustrative apparatus and methods for accomplishing in vivo electrofusion on corneas and in cervical areas.
Inventors
- 👤 Robert J. Grasso
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 01:13
CPC Classifications
A; A61; A61F; A61F9/00; A61F9/007; A61F9/013; C; C12; C12M; C12M35/00; C12M35/02; C12N; C12N13/00; C12N15/00; C12N15/02; C12N5/00; C12N5/10; C12N5/12
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
128 words
A device for confining flow of electrons through living tissue to a preselected area of said tissue, comprising: a first electrode means adapted to be disposed in abutting relation to a first preselected area of tissue; a second electrode means, generally annular in configuration, adapted to be disposed in abutting relation to a second preselected area of tissue having a generally spherical shape, said second preselected area of tissue being in closely spaced proximity to said first preselected area of tissue; a source of electrical power; means for electrically coupling said source of electrical power to said first electrode means; and means for grounding said second electrode means; whereby in vivo electrofusion occurs at the interface of said first preselected area of tissue and said first electrode means.
Claim 2
Independent Claim
128 words
A device for confining flow of electrons through living tissue to a preselected area of said tissue, comprising: a first electrode means adapted to be disposed in abutting relation to a first preselected area of tissue; a second electrode means, generally annular in configuration, adapted to be disposed in abutting relation to a second preselected area of tissue having a generally spherical shape, said second preselected area of tissue being in closely spaced proximity to said first preselected area of tissue; a source of electrical power; means for electrically coupling said source of electrical power to said first electrode means; and means for grounding said second electrode means; whereby in vivo electrofusion occurs at the interface of said first preselected area of tissue and said first electrode means.
Claim 3
Dependent Claim
15 words
The device of claim 1, wherein said source of electrical power is a pulse generator.
Claim 4
Dependent Claim
16 words
The device of claim 2, wherein said source of electrical power is a DC pulse generator.
Claim 5
Dependent Claim
26 words
The device of claim 3, wherein said first electrode means is configured and dimensioned to conform to the contour of said first preselected area of tissue.
Claim 6
Dependent Claim
37 words
The device of claim 4, wherein said first preselected area of tissue is the cornea of an eye and wherein said first electrode means is specifically configured and dimensioned to conform to the contour of said cornea.
Claim 7
Dependent Claim
25 words
The device of claim 5, wherein said second electrode means is specifically configured to conform to the contour of the sclera portion of an eye.
Claim 8
Dependent Claim
28 words
The device of claim 6, further comprising an insulated housing means that houses said first and second electrode means in a predetermined fixed spatial relationship to one another.
Claim 9
Independent Claim
129 words
A method of passing electrical current through preselected living tissue to accomplish in vivo electrofusion, comprising the steps of: depositing biological particles upon a first electrode means; positioning said first electrode means and said biological particles deposited thereupon in abutting relation to a first preselected area of tissue; configuring and dimensioning a tissue-contacting side of said first electrode means to conform to the contour of said tissue at said first preselected area; positioning a second electrode means in abutting relation to a second preselected area of tissue; configuring and dimensioning a tissue-contacting side of said second electrode means to conform to the contour of said tissue at said second preselected area; and imposing a preselected electrical potential difference between said first and second electrode means to accomplish said electrofusion.
Claim 10
Independent Claim
129 words
A method of passing electrical current through preselected living tissue to accomplish in vivo electrofusion, comprising the steps of: depositing biological particles upon a first electrode means; positioning said first electrode means and said biological particles deposited thereupon in abutting relation to a first preselected area of tissue; configuring and dimensioning a tissue-contacting side of said first electrode means to conform to the contour of said tissue at said first preselected area; positioning a second electrode means in abutting relation to a second preselected area of tissue; configuring and dimensioning a tissue-contacting side of said second electrode means to conform to the contour of said tissue at said second preselected area; and imposing a preselected electrical potential difference between said first and second electrode means to accomplish said electrofusion.
Claim 11
Dependent Claim
41 words
The method of claim 8, further comprising the step of spacing said first and second electrode means in close physical proximity to one another so that electrical current through areas of tissue remote from said preselected areas of tissue is limited.
Claim 12
Independent Claim
137 words
A method of accomplishing in vivo electrofusion of biological particles to an anatomical part of generally spherical configuration, comprising the steps of: depositing preselected biological particles upon a first electrode means; configuring and dimensioning said first electrode means to conform to the contour of a substantially central portion of said generally spherical anatomical part; positioning said first electrode means and said biological deposited thereupon in abutting, overlying relation to said substantially central portion of said generally spherical anatomical part; forming a second electrode means into a generally annular configuration; positioning said second electrode means into abutting relation to said anatomical part and in circumscribing relation to said first electrode means; and establishing an electrical potential difference between said first and second electrode means to thereby accomplish in vivo electrofusion of said biological particles to said anatomical part.
Claim 13
Independent Claim
137 words
A method of accomplishing in vivo electrofusion of biological particles to an anatomical part of generally spherical configuration, comprising the steps of: depositing preselected biological particles upon a first electrode means; configuring and dimensioning said first electrode means to conform to the contour of a substantially central portion of said generally spherical anatomical part; positioning said first electrode means and said biological deposited thereupon in abutting, overlying relation to said substantially central portion of said generally spherical anatomical part; forming a second electrode means into a generally annular configuration; positioning said second electrode means into abutting relation to said anatomical part and in circumscribing relation to said first electrode means; and establishing an electrical potential difference between said first and second electrode means to thereby accomplish in vivo electrofusion of said biological particles to said anatomical part.
Claim 14
Independent Claim
129 words
A method of accomplishing in vivo electrofusion of biological particles to the cornea of an eye, comprising the steps of: configuring and dimensioning a first electrode means to conform to the contour of a cornea; depositing biological particles upon said first electrode means; positioning said first electrode means and biological particles deposited thereupon into overlying relation to said cornea; configuring and dimensioning a second electrode means into a generally annular form, specifically into a form that conforms to the sclera portion of the eye; positioning said second electrode means into abutting, overlying relation to the sclera portion of said eye; and establishing an electrical potential difference between said first and second electrode means to thereby accomplish in vivo electrofusion of said biological particles to the cornea of an eye.
Claim 15
Independent Claim
129 words
A method of accomplishing in vivo electrofusion of biological particles to the cornea of an eye, comprising the steps of: configuring and dimensioning a first electrode means to conform to the contour of a cornea; depositing biological particles upon said first electrode means; positioning said first electrode means and biological particles deposited thereupon into overlying relation to said cornea; configuring and dimensioning a second electrode means into a generally annular form, specifically into a form that conforms to the sclera portion of the eye; positioning said second electrode means into abutting, overlying relation to the sclera portion of said eye; and establishing an electrical potential difference between said first and second electrode means to thereby accomplish in vivo electrofusion of said biological particles to the cornea of an eye.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-09-10 | 1994-03-12 | 1994-09-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-09-10 | 1998-03-12 | 1998-09-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-09-10 | 2002-03-12 | 2002-09-11 | $7700.00 | Window Closed / Lapsed |