US5670322A
Expired / Lapsed
US
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Multi site molecule detection method
Assignee: Baylor College of Medicine
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Filing: 1995-06-01
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Granted: 1997-09-23
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Expires: 2015-06-01
Abstract
Abstract A method and apparatus for identifying molecular structures within a sample substance using an array having a plurality of test sites upon which the sample substance is applied. Each test site includes a probe formed therein to bond with an associated target molecular structure. An electrical signal is applied to the test site and the electrical properties of the test sites are detected to determine which probes have bonded to an associated target molecular structure.
Inventors
- 👤 Mitchell D. Eggers
- 👤 Michael E. Hogan
- 👤 Kenneth Loren Beattie
- 👤 John Shumaker
- 👤 Daniel J. Ehrlich
- 👤 Mark Hollis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 13:24
CPC Classifications
B; B01; B01J; B01J19/00; B01J19/0046; B01J19/0093; B82; B82Y; B82Y30/00; C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/6825; C12Q1/6832; C12Q1/6834; C12Q1/6837; C12Q1/6869; C12Q1/6874; G; G01; G01N; G01N21/00; G01N21/17; G01N21/25; G01N21/251; G01N21/253; G01N27/00; G01N27/02; G01N27/22; G01N27/221; G01N27/226; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N33/5438; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00279; B01J2219/00306; B01J2219/00313; B01J2219/00315; B01J2219/00317; B01J2219/00497; B01J2219/00511; B01J2219/00527; B01J2219/00583; B01J2219/00585; B01J2219/00596; B01J2219/00603; B01J2219/00605; B01J2219/0061; B01J2219/00612; B01J2219/00614; B01J2219/00621; B01J2219/00623; B01J2219/00626; B01J2219/00632; B01J2219/00637; B01J2219/00653; B01J2219/00659; B01J2219/0068; B01J2219/00702; B01J2219/00709; B01J2219/00711; B01J2219/00713; B01J2219/00718; B01J2219/0072; B01J2219/00722; C40; C40B; C40B40/00; C40B40/04; C40B40/06; C40B60/00; C40B60/14; Y; Y10; Y10S; Y10S436/00; Y10S436/806; Y10S436/807; Y10S436/809
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
131 words
A method for identifying molecular structures within a sample substance, comprising the steps of: applying the substance to a plurality of test sites formed in a single substrate, said test sites having respective probes attached thereto which specifically bind to a target molecular structure, such that different test sites have probes which specifically bind to different target molecular structures; applying an electronic signal to the test sites at multiple frequencies with circuitry coupled to a plurality of electrodes associated with the test sites, each test site having at least one electrode attached thereto for electrical coupling with another electrode; detecting a frequency dependent electrical property of the test sites to determine which probes have bound to an associated target molecular structure such that a plurality of different targets can be detected.
Claim 2
Independent Claim
131 words
A method for identifying molecular structures within a sample substance, comprising the steps of: applying the substance to a plurality of test sites formed in a single substrate, said test sites having respective probes attached thereto which specifically bind to a target molecular structure, such that different test sites have probes which specifically bind to different target molecular structures; applying an electronic signal to the test sites at multiple frequencies with circuitry coupled to a plurality of electrodes associated with the test sites, each test site having at least one electrode attached thereto for electrical coupling with another electrode; detecting a frequency dependent electrical property of the test sites to determine which probes have bound to an associated target molecular structure such that a plurality of different targets can be detected.
Claim 3
Dependent Claim
11 words
The method of claim 1 wherein said probes are oligonucleotide probes.
Claim 4
Dependent Claim
11 words
The method of claim 1 wherein said probes are antibody probes.
Claim 5
Dependent Claim
11 words
The method of claim 1 wherein said probes are anti-antibody probes.
Claim 6
Dependent Claim
11 words
The method of claim 1 wherein said probes are peptide probes.
Claim 7
Dependent Claim
21 words
The method of claim 1 wherein said step of detecting comprises the step of detecting dielectric properties of the test site.
Claim 8
Dependent Claim
26 words
The method of claim 1 wherein said step of applying an electronic signal comprises the step of applying a signal over a predetermined range of frequency.
Claim 9
Dependent Claim
20 words
The method of claim 7 wherein said detecting step comprises the step of detecting a relaxation frequency within said range.
Claim 10
Dependent Claim
29 words
The method of claim 8 wherein said detecting step further comprises the step comparing said detected relaxation frequency to a known frequency for a test site with unbonded probes.
Claim 11
Dependent Claim
26 words
The method of claim 1 wherein said step of applying an electronic signal comprises applying a signal at predetermined discrete frequencies at a selected test site.
Claim 12
Dependent Claim
42 words
The method of claim 10 wherein said detecting step comprises measuring dispersion at said selected test site at each of said predetermined discrete frequencies and comparing the dispersion between said frequencies to determine whether bonding has occurred at said selected test site.
Claim 13
Dependent Claim
27 words
The method of claim 1 and further comprising the step of determining a molecular structure within the sample substance based on the test sites having bonded probes.
References Cited (Backward Citations)
US4072576A
Cited
US4096645A
Cited
US4293310A
Cited
US4414323A
Cited
US4414325A
Cited
US4786600A
Cited
US5064754A
Cited
US5164319A
Cited
US4822566A
Cited
US4728882A
Cited
US4935207A
Cited
US4963245A
Cited
US5071733A
Cited
US5001051A
Cited
US4769121A
Cited
US5082627A
Cited
DE3801987A1
Cited
US4938742A
Cited
EP0347579A2
Cited
WO1990002327A1
Cited
WO1990003382A1
Cited
WO1990005300A1
Cited
US5063081A
Cited
WO1990015070A1
Cited
EP0402917A2
Cited
US5194133A
Cited
US5187096A
Cited
US5532128A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-09-22 | 2001-03-24 | 2001-09-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-09-22 | 2005-03-24 | 2005-09-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-09-22 | 2009-03-24 | 2009-09-23 | $7700.00 | Window Closed / Lapsed |