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US5663064A
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US
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Ribozymes with RNA protein binding site
Assignee: University of Vermont
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Filing: 1995-01-13
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Granted: 1997-09-02
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Expires: 2015-01-13
Abstract
Abstract Ribozyme having a ligand binding site formed as a double-stranded RNA and a single-stranded loop, the ribozyme having enzymatic activity to cleave and/or ligate itself or a separate RNA molecule.
Inventors
- 👤 John M. Burke
- 👤 Bruno Sargueil
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-10 02:05
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/113; A; A61; A61K; A61K38/00; C12N2310/00; C12N2310/10; C12N2310/12; C12N2310/122; C12N2310/30; C12N2310/35; C12N2310/351; C12N2310/3513
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
65 words
An enzymatic RNA molecule comprising: a protein binding site formed as a double-stranded RNA and a single-stranded loop, wherein said enzymatic RNA molecule is capable of specifically cleaving a separate RNA molecule, wherein said site is selected from a group shown in FIGS. 2 and 12-17 (SEQ. ID. Nos. 4, 10-16 and 18-28) inclusive, and wherein said enzymatic nucleic acid is in a hairpin motif.
Claim 2
Dependent Claim
65 words
An enzymatic RNA molecule comprising: a protein binding site formed as a double-stranded RNA and a single-stranded loop, wherein said enzymatic RNA molecule is capable of specifically cleaving a separate RNA molecule, wherein said site is selected from a group shown in FIGS. 2 and 12-17 (SEQ. ID. Nos. 4, 10-16 and 18-28) inclusive, and wherein said enzymatic nucleic acid is in a hairpin motif.
Claim 3
Dependent Claim
24 words
The enzymatic RNA molecule of claim 1 or 4 wherein a protein is bound to said protein binding site in said enzymatic RNA molecule.
Claim 4
Dependent Claim
29 words
The enzymatic RNA molecule of claim 1 or 4 wherein the said protein binding site is adjacent to the 3' or the 5' end of said enzymatic RNA molecule.
Claim 5
Dependent Claim
64 words
An enzymatic RNA molecule comprising: a protein binding site formed as a double-stranded RNA and a single-stranded loop, wherein said enzymatic RNA molecule is capable of specifically ligating separate RNA molecules, wherein said site is selected from a group shown in FIGS. 2 and 12-17 (SEQ ID Nos. 4, 10-16 and 18-28) inclusive, and wherein said enzymatic nucleic acid is in a hairpin motif.
Claim 6
Dependent Claim
64 words
An enzymatic RNA molecule comprising: a protein binding site formed as a double-stranded RNA and a single-stranded loop, wherein said enzymatic RNA molecule is capable of specifically ligating separate RNA molecules, wherein said site is selected from a group shown in FIGS. 2 and 12-17 (SEQ ID Nos. 4, 10-16 and 18-28) inclusive, and wherein said enzymatic nucleic acid is in a hairpin motif.
Claim 7
Dependent Claim
20 words
The enzymatic RNA molecule of claim 1 or 4, wherein said protein binding site is capable of binding R17 protein.
Claim 8
Dependent Claim
24 words
The enzymatic RNA molecule of claim 1 or 4, wherein said protein binding site is capable of binding human immunodeficiency virus encoded TAT protein.
Claim 9
Dependent Claim
24 words
The enzymatic RNA molecule of claim 1 or 4, wherein said protein binding site is capable of binding human immunodeficiency virus encoded Rev protein.
Claim 10
Dependent Claim
24 words
The enzymatic RNA molecule of claim 1 or 4, wherein said protein binding site is capable of binding mammalian iron response element binding protein.
Claim 11
Dependent Claim
23 words
The enzymatic RNA molecule of claim 1 or 4, wherein said protein binding site is capable of binding mammalian vascular endothelial growth factor.
Claim 12
Dependent Claim
15 words
A mammalian cell in vitro including the enzymatic RNA molecule of claim 1 or 4.
Claim 13
Dependent Claim
32 words
An expression vector comprising nucleic acid encoding the enzymatic RNA molecule of claim 1 or 4, in a manner which allows expression of that enzymatic RNA molecule within a cell in vitro.
Claim 14
Dependent Claim
13 words
A mammalian cell in vitro including an the expression vector of claim 11.
Claim 15
Independent Claim
33 words
A method for increasing the activity of a an enzymatic RNA molecule in a hairpin motif by including a RNA protein binding site in the helix 4 region of said enzymatic RNA molecule.
Claim 16
Independent Claim
33 words
A method for increasing the activity of a an enzymatic RNA molecule in a hairpin motif by including a RNA protein binding site in the helix 4 region of said enzymatic RNA molecule.
Claim 17
Independent Claim
20 words
A method for decreasing the activity of a hairpin ribozyme by including a RNA protein binding site in helix 4.
Claim 18
Independent Claim
20 words
A method for decreasing the activity of a hairpin ribozyme by including a RNA protein binding site in helix 4.
Claim 19
Independent Claim
32 words
A method for localizing an enzymatic RNA molecule in a hairpin motif by providing a RNA protein binding sequence in said enzymatic RNA molecule and binding said site with a localizing protein.
Claim 20
Independent Claim
32 words
A method for localizing an enzymatic RNA molecule in a hairpin motif by providing a RNA protein binding sequence in said enzymatic RNA molecule and binding said site with a localizing protein.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 2000-09-01 | 2001-03-03 | 2001-09-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-09-01 | 2005-03-03 | 2005-09-02 | $3760.00 | Window Closed / Lapsed |
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