US5459015A
Expired / Lapsed
US
⏱️ 0.0 years remaining
High-affinity RNA ligands of basic fibroblast growth factor
Assignee: Gilead Sciences Inc
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Filing: 1994-02-10
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Granted: 1995-10-17
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Expires: 2014-02-10
Abstract
Abstract Methods are described for the identification and preparation of nucleic acid ligands and ligand solutions to basic fibroblast growth factor (bFGF). Included in the invention are nucleic acid ligands to bFGF which are inhibitors of bFGF and 2'-amino-modified RNA ligands to bFGF.
Inventors
- 👤 Nebojsa Janjic
- 👤 Larry Gold
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-10 02:07
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/115; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/54; A61K47/547; A61K47/549; B; B82; B82Y; B82Y5/00; C07; C07H; C07H19/00; C07H19/02; C07H19/04; C07H19/06; C07H19/10; C07H21/00; C07K; C07K14/00; C07K14/001; C12N15/10; C12N15/1034; C12N15/1048; C12N15/113; C12N15/1136; C12N9/00; C12N9/10; C12N9/12; C12N9/1241; C12N9/1276; C12Q; C12Q1/00; C12Q1/34; C12Q1/37; C12Q1/68; C12Q1/6811; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; G01N33/569; G01N33/56983; G01N33/56988; G01N33/68; G01N33/74; G01N33/76; C12N2310/00; C12N2310/10; C12N2310/13; C12N2310/30; C12N2310/32; C12N2310/322; C12N2310/50; C12N2310/53; C40; C40B; C40B40/00; F; F02; F02B; F02B75/00; F02B75/02; F02B2075/022; F02B2075/027; G01N2333/00; G01N2333/005; G01N2333/08; G01N2333/15; G01N2333/155; G01N2333/16; G01N2333/163; G01N2333/435; G01N2333/475; G01N2333/50; G01N2333/503; G01N2333/575; G01N2333/62; G01N2333/81; G01N2333/8107; G01N2333/811; G01N2333/8121; G01N2333/8125; G01N2333/90; G01N2333/914; G01N2333/948; G01N2333/95; G01N2333/964; G01N2333/96425; G01N2333/96427; G01N2333/9643; G01N2333/96433; G01N2333/96436; G01N2333/96441; G01N2333/96455; G01N2333/96486; G01N2333/966; G01N2333/972; G01N2333/9726; G01N2333/974; G01N2333/976
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
106 words
A method for identifying nucleic acid ligands to basic fibroblast growth factor (bFGF) comprising: a) preparing a candidate mixture of nucleic acids, b) contacting the candidate mixture with bFGF, wherein nucleic acids having sequences with an increased affinity to bFGF relative to the total candidate mixture may be partitioned from the remainder of the nucleic acids in the candidate mixture, c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture, and d) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for sequences with increased affinity to bFGF, whereby nucleic acid ligands to bFGF may be identified.
Claim 2
Independent Claim
106 words
A method for identifying nucleic acid ligands to basic fibroblast growth factor (bFGF) comprising: a) preparing a candidate mixture of nucleic acids, b) contacting the candidate mixture with bFGF, wherein nucleic acids having sequences with an increased affinity to bFGF relative to the total candidate mixture may be partitioned from the remainder of the nucleic acids in the candidate mixture, c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture, and d) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for sequences with increased affinity to bFGF, whereby nucleic acid ligands to bFGF may be identified.
Claim 3
Dependent Claim
13 words
The method of claim 1 further comprising e) repeating steps b) through d).
Claim 4
Dependent Claim
19 words
The method of claim 1 wherein said candidate mixture of nucleic acids is comprised of single stranded nucleic acids.
Claim 5
Dependent Claim
16 words
The method of claim 3 wherein said candidate mixture of nucleic acids is comprised of RNA.
Claim 6
Dependent Claim
23 words
The method of claim 4 wherein said candidate mixture of nucleic acids is comprised of RNA wherein all pyrimidines are 2'-deoxy-2-NH 2 pyrimidines.
Claim 7
Dependent Claim
48 words
A method for selecting a nucleic acid ligand which inhibits the binding of bFGF to its receptor, which comprises the additional step of screening nucleic acid ligands to bFGF identified by the method of claim 1 for their ability to inhibit the binding of bFGF to its receptor.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-10-16 | 1999-04-17 | 1999-10-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-10-16 | 2003-04-17 | 2003-10-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-10-16 | 2007-04-17 | 2007-10-17 | $7700.00 | Window Closed / Lapsed |