US5660692A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of crosslinking amino acid-containing polymers using photoactivatable chemical crosslinkers
Assignee: Cedars-Sinai Medical Center
•
Filing: 1995-06-07
•
Granted: 1997-08-26
•
Expires: 2015-06-07
Abstract
Abstract A method for molecularly crosslinking amino acid-containing polymers by photoactivating chemical crosslinkers which have been combined with the polymers. Collagen crosslinked by this method can be used as a bioadhesive for sutureless closures of the skin and eye or as a superhydrated material for contact lenses, moist bandage contact lens, lens or corneal implant material, or as a drug delivery mechanism.
Inventors
- 👤 Anthony B. Nesburn
- 👤 Michael B. Gorin
- 👤 M. Cristina Kenney
- 👤 Ezra Maguen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:04
CPC Classifications
A; A61; A61L; A61L24/00; A61L24/04; A61L24/10; A61L24/102; A61L27/00; A61L27/14; A61L27/22; A61L27/24; C; C07; C07K; C07K14/00; C07K14/435; C07K14/78; C08; C08H; C08H1/00; C08H1/06; G; G03; G03F; G03F7/00; G03F7/004; G03F7/038; A61F; A61F2310/00; A61F2310/00005; A61F2310/00365; A61K; A61K38/00
Claims Analysis
Total Claims: 30
Claim 1
Independent Claim
118 words
A method of coupling amino acid-containing polymers, said method comprising the steps of: providing a first collagen polymer and a second collagen polymer, with the proviso that the first collagen polymer and the second collagen polymer are of different collagens; combining the first collagen polymer, the second collagen polymer, and a photoactivatable, heterobifunctional crosslinking agent having at least one photoactivatable site and at least one conventional site, wherein at least one said conventional site is coupled to at least one of said first collagen polymer and said second collagen polymer; and photoactivating the crosslinking agent, wherein at least one said photoactivatable site is coupled to the other of said first and said second collagen polymers in the combination.
Claim 2
Independent Claim
118 words
A method of coupling amino acid-containing polymers, said method comprising the steps of: providing a first collagen polymer and a second collagen polymer, with the proviso that the first collagen polymer and the second collagen polymer are of different collagens; combining the first collagen polymer, the second collagen polymer, and a photoactivatable, heterobifunctional crosslinking agent having at least one photoactivatable site and at least one conventional site, wherein at least one said conventional site is coupled to at least one of said first collagen polymer and said second collagen polymer; and photoactivating the crosslinking agent, wherein at least one said photoactivatable site is coupled to the other of said first and said second collagen polymers in the combination.
Claim 3
Dependent Claim
14 words
The method of claim 1, wherein said first collagen polymer is collagen type I.
Claim 4
Dependent Claim
27 words
The method of claim 2, wherein said collagen type I is selected from the group consisting of bovine collagen type I and human placental collagen type I.
Claim 5
Dependent Claim
32 words
The method of claim 2, wherein said second collagen polymer is collagen type V, and wherein the ratio of the collagen type I to the collagen type V is 10:1 or less.
Claim 6
Dependent Claim
14 words
The method of claim 1, wherein said second collagen polymer is collagen type V.
Claim 7
Dependent Claim
15 words
The method of claim 5, wherein said collagen type V is human collagen type V.
Claim 8
Dependent Claim
27 words
The method of claim 1, wherein the photoactivatable, heterobifunctional crosslinking agent is selected from the group consisting of diazo compounds, aryl azide compounds, and alkyl azide compounds.
Claim 9
Dependent Claim
46 words
The method of claim 1, wherein said first collagen polymer is selected from the group consisting of human collagen types I, IV, V, and IX, and wherein said second collagen polymer is selected from the group consisting of nonhuman collagen types I, IV, V, and IX.
Claim 10
Dependent Claim
42 words
The method of claim 1, further comprising the step of combining said first and said second collagen polymers with a gelatin prior to photoactivating the crosslinking agent, said gelatin selected from the group consisting of fish skin gelatin and calf skin gelatin.
Claim 11
Dependent Claim
13 words
A coupled amino acid-containing polymer produced by the process defined in claim 1.
Claim 12
Dependent Claim
97 words
A coupled amino acid-containing polymer composition comprising: a first collagen polymer; a second collagen polymer; and a photoactivatable heterobifunctional crosslinking agent having at least one photoactivatable site and at least one conventional site, wherein at least one said photoactivatable site is coupled to at least one of said first collagen polymer and said second collagen polymer, and wherein at least one said conventional site is coupled to the other of said first and said second collagen polymers in the combination, with the proviso that the first collagen polymer and the second collagen polymer are of different collagens.
Claim 13
Dependent Claim
97 words
A coupled amino acid-containing polymer composition comprising: a first collagen polymer; a second collagen polymer; and a photoactivatable heterobifunctional crosslinking agent having at least one photoactivatable site and at least one conventional site, wherein at least one said photoactivatable site is coupled to at least one of said first collagen polymer and said second collagen polymer, and wherein at least one said conventional site is coupled to the other of said first and said second collagen polymers in the combination, with the proviso that the first collagen polymer and the second collagen polymer are of different collagens.
Claim 14
Dependent Claim
15 words
T The composition of Claim 11, wherein said first collagen polymer is collagen type I.
Claim 15
Dependent Claim
32 words
The composition of claim 12, wherein said second collagen polymer is collagen type V, and wherein the ratio of the collagen type I to the collagen type V is 10:1 or less.
Claim 16
Dependent Claim
27 words
The composition of claim 12, wherein said collagen type I is selected from the group consisting of bovine collagen type I and human placental collagen type I.
Claim 17
Dependent Claim
14 words
The composition of claim 11, wherein said second collagen polymer is collagen type V.
Claim 18
Dependent Claim
15 words
The composition of claim 15, wherein said collagen type V is human collagen type V.
Claim 19
Dependent Claim
27 words
The composition of claim 11, wherein the photoactivatable, heterobifunctional crosslinking agent is selected from the group consisting of diazo compounds, aryl azide compounds, and alkyl azide compounds.
Claim 20
Dependent Claim
46 words
The composition of claim 11, wherein said first collagen polymer is selected from the group consisting of human collagen types I, IV, V, and IX, and wherein said second collagen polymer is selected from the group consisting of nonhuman collagen types I, IV, V, and IX.
Claim 21
Dependent Claim
42 words
The composition of claim 11, further comprising the step of combining said first and said second collagen polymers with a gelatin prior to photoactivating the crosslinking agent, said gelatin selected from the group consisting of fish skin gelatin and calf skin gelatin.
Claim 22
Independent Claim
87 words
A method of coupling amino acid-containing polymers, said method comprising the steps of: combining an amino acid-containing polymer, a proteoglycan, and a photoactivatable, heterobifunctional crosslinking agent having at least one photoactivatable site and at least one conventional site, wherein at least one said conventional site is coupled to at least one of said amino acid-containing polymer and said proteoglycan; and photoactivating the crosslinking agent, wherein at least one said photoactivatable site is coupled to the other of said amino acid-containing polymer and said proteoglycan in the combination.
Claim 23
Independent Claim
87 words
A method of coupling amino acid-containing polymers, said method comprising the steps of: combining an amino acid-containing polymer, a proteoglycan, and a photoactivatable, heterobifunctional crosslinking agent having at least one photoactivatable site and at least one conventional site, wherein at least one said conventional site is coupled to at least one of said amino acid-containing polymer and said proteoglycan; and photoactivating the crosslinking agent, wherein at least one said photoactivatable site is coupled to the other of said amino acid-containing polymer and said proteoglycan in the combination.
Claim 24
Dependent Claim
12 words
The method of claim 20, wherein said amino acid-containing polymer is collagen.
Claim 25
Dependent Claim
22 words
The method of claim 21, wherein said collagen is selected from the group consisting of collagen type I, IV, V, and IX.
Claim 26
Dependent Claim
13 words
A coupled amino acid-containing polymer produced by the process defined in claim 20.
Claim 27
Dependent Claim
76 words
A coupled amino acid-containing polymer composition comprising: an amino acid-containing polymer; a proteoglycan; and a photoactivatable heterobifunctional crosslinking agent having at least one photoactivatable site and at least one conventional site, wherein at least one said photoactivatable site is coupled to at least one of said amino acid-containing polymer and said proteoglycan, and wherein at least one said conventional site is coupled to the other of said amino acid-containing polymer and said proteoglycan in the combination.
Claim 28
Dependent Claim
76 words
A coupled amino acid-containing polymer composition comprising: an amino acid-containing polymer; a proteoglycan; and a photoactivatable heterobifunctional crosslinking agent having at least one photoactivatable site and at least one conventional site, wherein at least one said photoactivatable site is coupled to at least one of said amino acid-containing polymer and said proteoglycan, and wherein at least one said conventional site is coupled to the other of said amino acid-containing polymer and said proteoglycan in the combination.
Claim 29
Dependent Claim
12 words
The composition of claim 24, wherein said amino acid-containing polymer is collagen.
Claim 30
Dependent Claim
22 words
The composition of claim 25, wherein said collagen is selected from the group consisting of collagen type I, IV, V, and IX.
References Cited (Backward Citations)
GB1015284A
Cited
US4505855A
Cited
US4621631A
Cited
US4511478A
Cited
US4597999A
Cited
US4883487A
Cited
EP0246013A2
Cited
US5024742A
Cited
US5294314A
Cited
US5431790A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-08-25 | 2001-02-24 | 2001-08-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-08-25 | 2005-02-24 | 2005-08-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-08-25 | 2009-02-24 | 2009-08-26 | $7700.00 | Window Closed / Lapsed |