US4883487A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of producing an artifical skin
Assignee: Koken Co Ltd
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Filing: 1987-04-06
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Granted: 1989-11-28
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Expires: 2007-04-06
Abstract
Abstract An artifical skin comprising an insoluble atelocollagen sheet and an epidermal cell layer cultured only on one surface of the sheet, and a method of producing the same are disclosed, which method includes the steps of: forming an insoluble atelocollagen sheet; inoculating epidermal cells only on one surface of the atelocollagen sheet; and suspending the atelocollagen sheet in a liquid culture medium to culture the epidermal cells, thereby forming an epidermal cell layer.
Inventors
- 👤 Katsutoshi Yoshizato
- 👤 Toshio Taira
- 👤 Teruo Miyata
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 04:57
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/14; A61L27/22; A61L27/24; A61F; A61F2/00; A61F2/02; A61F2/10; A61F2/105; A61L27/50; A61L27/60; A61F2310/00; A61F2310/00005; A61F2310/00365; Y; Y10; Y10S; Y10S623/00; Y10S623/901
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
88 words
A method of producing an artificial skin comprising: (a) pouring an acidic atelocollagen solution into a mold to a depth of 1 to 5 mm; (b) air-drying said poured atelocollagen solution to form a dry sheet; (c) cross-linking said dry sheet by ultraviolet irradiation for a time sufficient to form an insoluble atelocollagen sheet; (d) inoculating epidermal cells on one surface of said atelocollagen sheet; and (e) suspending the inoculated atelocollagen sheet in a liquid culture medium to culture said epidermal cells, thereby forming an epidermal cell layer.
Claim 2
Independent Claim
88 words
A method of producing an artificial skin comprising: (a) pouring an acidic atelocollagen solution into a mold to a depth of 1 to 5 mm; (b) air-drying said poured atelocollagen solution to form a dry sheet; (c) cross-linking said dry sheet by ultraviolet irradiation for a time sufficient to form an insoluble atelocollagen sheet; (d) inoculating epidermal cells on one surface of said atelocollagen sheet; and (e) suspending the inoculated atelocollagen sheet in a liquid culture medium to culture said epidermal cells, thereby forming an epidermal cell layer.
Claim 3
Dependent Claim
26 words
A method according to claim 1, further comprising culturing fibroblasts on at least one surface of said insoluble altelocollagen sheet prior to inoculating with epidermal cells.
Claim 4
Dependent Claim
19 words
A method according top claim 1, wherein the concentration of atelocollagen in the poured solution is 0.3 to 1.0%.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-11-27 | 1993-05-29 | 1993-11-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-11-27 | 1997-05-29 | 1997-11-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-11-27 | 2001-05-29 | 2001-11-28 | $7700.00 | Window Closed / Lapsed |