US4495288A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of culturing anchorage dependent cells
Assignee: Abbott Biotech Inc
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Filing: 1983-04-13
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Granted: 1985-01-22
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Expires: 2003-04-13
Abstract
Abstract Disclosed is a method of growing anchorage-dependent cells: cells of the type which normally undergo mitosis only when anchored on a substrate, e.g., fibroblasts or epithelial cells. The method comprises the steps of encapsulating a seed culture of the cells within a semipermeable membrane and suspending the capsules in a growth medium. The interior surfaces of the capsule membrane and/or collagen enclosed within the capsules serve as a substrate for the cells. The ratio of the available substrate surface area to the volume of the culture may be large, thereby allowing the cells to be grown substantially throughout the volume of the culture medium.
Inventors
- 👤 Allan P. Jarvis, Jr.
- 👤 Franklin Lim
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:20
CPC Classifications
C; C12; C12P; C12P21/00; C12P21/02; C12N; C12N5/00; C12N5/0012; C12N2533/00; C12N2533/50; C12N2533/54; C12N2533/70; C12N2533/74; Y; Y10; Y10S; Y10S623/00; Y10S623/915
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
143 words
A process for culturing anchorage dependent cells, said process comprising the steps of: A. suspending a cell in a medium containing an anchorage substrate material and high molecular weight components needed to maintain viability and to support mitosis of said cell; B. encapsulating said cell together with said medium and anchorage substrate material within a semipermeable membrane comprising a polysaccharide having plural anionic groups cross-linked with a polymer having plural cationic groups and a molecular weight greater than 3,000, said semipermeable membrane having an upper limit of permeability sufficient to preclude traverse of said anchorage substrate material and sufficient to allow low molecular weight molecules to traverse said membrane; C. suspending the product of step B in a culture medium sufficient to maintain viability and to support mitosis of said encapsulated cell; and D. allowing said cell to undergo mitosis within said membrane.
Claim 2
Dependent Claim
143 words
A process for culturing anchorage dependent cells, said process comprising the steps of: A. suspending a cell in a medium containing an anchorage substrate material and high molecular weight components needed to maintain viability and to support mitosis of said cell; B. encapsulating said cell together with said medium and anchorage substrate material within a semipermeable membrane comprising a polysaccharide having plural anionic groups cross-linked with a polymer having plural cationic groups and a molecular weight greater than 3,000, said semipermeable membrane having an upper limit of permeability sufficient to preclude traverse of said anchorage substrate material and sufficient to allow low molecular weight molecules to traverse said membrane; C. suspending the product of step B in a culture medium sufficient to maintain viability and to support mitosis of said encapsulated cell; and D. allowing said cell to undergo mitosis within said membrane.
Claim 3
Dependent Claim
12 words
The process of claim 1 wherein said anchorage substrate is a protein.
Claim 4
Dependent Claim
11 words
The process of claim 1 wherein said anchorage substrate is collagen.
Claim 5
Dependent Claim
29 words
The process of claim 1 wherein the anchorage substrate is calf skin collagen and is included in said suspension at a concentration between about 10 ug/ml and 1.0 mg/ml.
Claim 6
Dependent Claim
21 words
The process of claim 1 comprising the additional step of selectively disrupting said membranes after step D to release said cells.
Claim 7
Dependent Claim
11 words
The process of claim 1 wherein said cell comprises a fibroblast.
Claim 8
Dependent Claim
14 words
The process of claim 1 wherein said high molecular weight components comprise serum components.
Claim 9
Dependent Claim
16 words
The process of claim 1 wherein said cell comprises a human fibroblast capable of secreting interferon.
Claim 10
Dependent Claim
24 words
The process of claim 1 wherein during said encapsulation step spheroidal membranes having an average diameter in the range of 100-500 microns are produced.
Claim 11
Dependent Claim
17 words
The process of claim 1 wherein said anchorage substrate comprises a protein having plural free cationic groups.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-01-22 | 1988-07-23 | 1989-01-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-01-22 | 1992-07-23 | 1993-01-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-01-22 | 1996-07-23 | 1997-01-22 | $7700.00 | Window Closed / Lapsed |