US4485096A
Expired / Lapsed
US
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Tissue-equivalent and method for preparation thereof
Assignee: Massachusetts Institute of Technology
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Filing: 1982-05-26
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Granted: 1984-11-27
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Expires: 2002-05-26
Abstract
Abstract A tissue-equivalent, useful in the treatment of burns or other skin wounds and in the fabrication of prostheses, is disclosed which is prepared from a hydrated collagen lattice contracted by a contractile agent, such as fibroblast cells or blood platelets, to form tissue-equivalent. In one embodiment, a skin-equivalent can be fabricated by growing a layer of keratinocyte cells thereon. Both the keratinocyte cells and contractile agent may be derived from the potential recipient of the skin-equivalent.
Inventors
- 👤 Eugene Bell
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-22 22:05
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/36; A61L27/3604; A61L27/3608; A61F; A61F2/00; A61F2/02; A61F2/28; A61L27/14; A61L27/22; A61L27/24; A61L27/3641; A61L27/3645; A61L27/365; A61L27/38; A61L27/40; A61L27/44; A61F2310/00; A61F2310/00005; A61F2310/00365; A61L2430/00; A61L2430/02; Y; Y10; Y10S; Y10S623/00; Y10S623/915; Y10S623/916; Y10S623/917
Claims Analysis
Total Claims: 38
Claim 1
Independent Claim
57 words
A method of producing a tissue-equivalent, comprising: a. combining a collagen solution with a contractile agent under conditions to form a gel mixture having said contractile agent dispersed within said gel mixture; and then b. maintaining the gel mixture prepared in step (a) under conditions which permit the contraction of said gel mixture to form a tissue-equivalent.
Claim 2
Independent Claim
57 words
A method of producing a tissue-equivalent, comprising: a. combining a collagen solution with a contractile agent under conditions to form a gel mixture having said contractile agent dispersed within said gel mixture; and then b. maintaining the gel mixture prepared in step (a) under conditions which permit the contraction of said gel mixture to form a tissue-equivalent.
Claim 3
Dependent Claim
16 words
A method of claim 1 additionally including adding living tissue cells into or onto said tissue-equivalent.
Claim 4
Dependent Claim
14 words
A method of claim 1 or 2 wherein said contractile agent comprises fibroblast cells.
Claim 5
Dependent Claim
14 words
A method of claim 1 or 2 wherein said contractile agent comprises blood platelets.
Claim 6
Dependent Claim
15 words
A method of claim 1 or 2 wherein said contractile agent comprises smooth muscle cells.
Claim 7
Dependent Claim
15 words
A method of claim 1 or 2 wherein said contractile agent comprises striated muscle cells.
Claim 8
Independent Claim
15 words
A method of claim, 1 or 2 wherein said contractile agent comprises heart muscle cells.
Claim 9
Dependent Claim
10 words
A tissue-equivalent formed according to the method of claim 1.
Claim 10
Dependent Claim
10 words
A tissue-equivalent formed according to the method of claim 2.
Claim 11
Independent Claim
79 words
A method of preparing a living tissue-equivalent, comprising: a. forming an acidic solution of collagen; b. combining contractile cells and nutrient medium with said acidic solution of collagen; c. raising the pH of said solution of collagen to a level sufficient to precipitate collagen fibrils into a hydrated collagen lattice containing said contractile cells; and d. maintaining said lattice and said contractile cells under conditions sufficient for said cells to contract said collagen lattice thereby forming a living tissue-equivalent.
Claim 12
Independent Claim
79 words
A method of preparing a living tissue-equivalent, comprising: a. forming an acidic solution of collagen; b. combining contractile cells and nutrient medium with said acidic solution of collagen; c. raising the pH of said solution of collagen to a level sufficient to precipitate collagen fibrils into a hydrated collagen lattice containing said contractile cells; and d. maintaining said lattice and said contractile cells under conditions sufficient for said cells to contract said collagen lattice thereby forming a living tissue-equivalent.
Claim 13
Dependent Claim
13 words
A method of claim 10 wherein steps b and c are done simultaneously.
Claim 14
Dependent Claim
10 words
A living tissue-equivalent formed by the method of claim 11.
Claim 15
Independent Claim
75 words
A method of forming skin-equivalent, comprising: a. forming a hydrated collagen lattice incorporating a living cellular contractile agent therein; b. maintaining said lattice and said contractile agent under conditions sufficient for said agent to contract said collagen lattice to form living dermal-equivalent; c. plating keratinocyte cells upon said dermal-equivalent; and d. maintaining said dermal equivalent under conditions sufficient for growth of said living cellular contractile agent and said keratinocyte cells to thereby produce a skin-equivalent.
Claim 16
Independent Claim
75 words
A method of forming skin-equivalent, comprising: a. forming a hydrated collagen lattice incorporating a living cellular contractile agent therein; b. maintaining said lattice and said contractile agent under conditions sufficient for said agent to contract said collagen lattice to form living dermal-equivalent; c. plating keratinocyte cells upon said dermal-equivalent; and d. maintaining said dermal equivalent under conditions sufficient for growth of said living cellular contractile agent and said keratinocyte cells to thereby produce a skin-equivalent.
Claim 17
Dependent Claim
24 words
A skin-equivalent, comprising: a. a hydrated collagen lattice contracted with a contractile agent; and b. keratinocyte cells supported upon said contracted hydrated collagen lattice.
Claim 18
Dependent Claim
24 words
A skin-equivalent, comprising: a. a hydrated collagen lattice contracted with a contractile agent; and b. keratinocyte cells supported upon said contracted hydrated collagen lattice.
Claim 19
Independent Claim
79 words
A method of treating a wound to the skin of a donor, comprising: a. culturing fibroblast cells, in vitro, said fibroblast cells being derived from the donor; b. forming a hydrated collagen lattice incorpotating said cultured fibroblast cells and maintaining said lattice and said cells under conditions whereby said fibroblast cells attach to said collagen lattice and contract it into a living tissue-equivalent; and c. transplanting said living tissue-equivalent as a graft at the wound site of said donor.
Claim 20
Independent Claim
79 words
A method of treating a wound to the skin of a donor, comprising: a. culturing fibroblast cells, in vitro, said fibroblast cells being derived from the donor; b. forming a hydrated collagen lattice incorpotating said cultured fibroblast cells and maintaining said lattice and said cells under conditions whereby said fibroblast cells attach to said collagen lattice and contract it into a living tissue-equivalent; and c. transplanting said living tissue-equivalent as a graft at the wound site of said donor.
Claim 21
Dependent Claim
20 words
A method of claim 15 additionally including growing a layer of keratinocytes upon said living tissue-equivalent prior to transplanting it.
Claim 22
Independent Claim
47 words
A method of forming a biocompatible prosthesis for a potential recipient, comprising: a. forming a hydrated collagen lattice incorporating a contractile agent obtained from said potential recipient; and b. maintaining said lattice and contractile agent under conditions sufficient to contract the hydrated collagen lattice into biocompatible prosthesis.
Claim 23
Independent Claim
47 words
A method of forming a biocompatible prosthesis for a potential recipient, comprising: a. forming a hydrated collagen lattice incorporating a contractile agent obtained from said potential recipient; and b. maintaining said lattice and contractile agent under conditions sufficient to contract the hydrated collagen lattice into biocompatible prosthesis.
Claim 24
Independent Claim
55 words
A method of forming a biocompatible prosthesis for a potential recipient, comprising: a. forming a hydrated collagen lattice incorporating a contractile agent obtained from a donor other than said potential recipient; and b. maintaining said lattice and contractile agent under conditions sufficient for the agent to contract the hydrated collagen lattice into a biocompatible prosthesis.
Claim 25
Independent Claim
55 words
A method of forming a biocompatible prosthesis for a potential recipient, comprising: a. forming a hydrated collagen lattice incorporating a contractile agent obtained from a donor other than said potential recipient; and b. maintaining said lattice and contractile agent under conditions sufficient for the agent to contract the hydrated collagen lattice into a biocompatible prosthesis.
Claim 26
Dependent Claim
23 words
A method of claim 18 wherein said contractile agent comprises fibroblast cells maintained under conditions which eliminate immune response in said potential recipient.
Claim 27
Dependent Claim
15 words
A method of claim 17 or claim 19 wherein said contractile agent comprises fibroblast cells.
Claim 28
Dependent Claim
15 words
A method of claim 17 or claim 19 wherein said contractile agent comprises blood platelets.
Claim 29
Dependent Claim
16 words
A method of claim 17 or claim 19 wherein said contractile agent comprises smooth muscle cells.
Claim 30
Dependent Claim
16 words
A method of claim 17 or claim 19 wherein said contractile agent comprises striated muscle cells.
Claim 31
Dependent Claim
16 words
A method of claim 17 or claim 19 wherein said contractile agent comprises heart muscle cells.
Claim 32
Dependent Claim
22 words
An organ-equivalent, comprising: a. a hydrated collagen lattice contracted with fibroblast cells incorporated therein; and b. living organ cells thereon or therein.
Claim 33
Dependent Claim
22 words
An organ-equivalent, comprising: a. a hydrated collagen lattice contracted with fibroblast cells incorporated therein; and b. living organ cells thereon or therein.
Claim 34
Dependent Claim
12 words
An organ-equivalent of claim 25 wherein said organ cells comprise heart cells.
Claim 35
Dependent Claim
12 words
An organ-equivalent of claim 25 wherein said organ cells comprise liver cells.
Claim 36
Dependent Claim
22 words
A gland-equivalent, comprising: a. a hydrated collagen lattice contracted with fibroblast cells incorporated therein; and b. living glandular cells thereon or therein.
Claim 37
Dependent Claim
22 words
A gland-equivalent, comprising: a. a hydrated collagen lattice contracted with fibroblast cells incorporated therein; and b. living glandular cells thereon or therein.
Claim 38
Dependent Claim
13 words
A gland-equivalent of claim 28 wherein said glandular cells comprise insulin-producing pancreatic cells.
References Cited (Backward Citations)
US4314380A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-11-27 | 1988-05-28 | 1988-11-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-11-27 | 1992-05-28 | 1992-11-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-11-27 | 1996-05-28 | 1996-11-27 | $7700.00 | Window Closed / Lapsed |