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EP0246013A2
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Method of making collagen film
Assignee: Koken Co Ltd
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Filing: 1987-05-01
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Granted: —
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Expires: 2007-05-01
Abstract
Abstract A method of producing a transparent collagen film having a high permeability to materials and suitable for culturing animal cells, comprises the steps of: forming a collagen film from an aqueous acid solution containing a collagen; dipping the collagen sheet in a saline solution; and radiating ultraviolet rays onto the collagen sheet.
Inventors
- 👤 Katsutoshi Yoshizato
- 👤 Toshio Taira
- 👤 Teruo Miyata
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-24 05:35
CPC Classifications
C; C08; C08J; C08J3/00; C08J3/28; A; A61; A61F; A61F2/00; A61F2/02; A61F2/10; A61L; A61L27/00; A61L27/14; A61L27/22; A61L27/24; A61L27/36; C08H; C08H1/00; C08H1/06; C08J5/00; C08J5/18; C12; C12N; C12N5/00; C12N5/0068; A61F2310/00; A61F2310/00005; A61F2310/00365; C08J2389/00; C08J2389/04; C08J2389/06; C12N2533/00; C12N2533/50; C12N2533/54
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
20 words
A method of producing collagen film comprising contacting collagen sheet with saline solution and subjecting the sheet to ultraviolet irradiation.
Claim 2
Independent Claim
20 words
A method of producing collagen film comprising contacting collagen sheet with saline solution and subjecting the sheet to ultraviolet irradiation.
Claim 3
Dependent Claim
21 words
A method according to claim 1 wherein the saline solution is a phosphoric acid-buffered saline solution of pH 5 to 9.
Claim 4
Dependent Claim
17 words
A method according to claim 1 or 2 wherein the collagen is acid soluble collagen or atelocollagen.
Claim 5
Independent Claim
21 words
A method according to any of claims 1 to 3 wherein the collagen sheet is formed from aqueous acid containing collagen.
Claim 6
Dependent Claim
17 words
A method according to claim 4 wherein the aqueous acid is an aqueous solution of hydrochloric acid.
Claim 7
Independent Claim
20 words
A method of culturing cells in which a film made according to any preceding claim is employed as culture medium.
References Cited (Backward Citations)
No citation records indexed.
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