US5612487A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Anti-viral vaccines expressed in plants
Assignee: TERRAMED Inc
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Filing: 1993-03-04
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Granted: 1997-03-18
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Expires: 2013-03-04
Abstract
Abstract The anti-viral vaccine of the present invention is produced in transgenic plants and then administered through standard vaccine introduction method or through the consumption of the edible portion of those plants. A DNA sequence encoding for the expression of a surface antigen of a viral pathogen is isolated and ligated to a promoter which can regulate the production of the surface antigen in a transgenic plant. This gene is then transferred to plant cells using a procedure that results in its integration into the plant genome, such as through the use of an Agrobacterium tumenfaciens plasmid vector system. Preferably, the foreign gene is expressed in an portion of the plant that is edible by humans or animals. In a preferred procedure, the vaccine is administered through the consumption of the edible plant as food, preferably in the form of a fruit or vegetable juice which can be taken orally.
Inventors
- 👤 Dominic Man-Kit Lam
- 👤 Charles J. Arntzen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 14:35
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; A; A61; A61P; A61P31/00; A61P31/12; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8241; C12N15/8242; C12N15/8257; C12N15/8258; A61K; A61K39/00; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/40; C07K2319/61; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
37 words
A transgenic tobacco plant comprising a DNA sequence encoding a recombinant hepatitis B viral surface antigen protein, wherein said plant is capable of synthesizing a recombinant hepatitis B viral surface antigen protein which assembles into antigenic particles.
Claim 2
Dependent Claim
37 words
A transgenic tobacco plant comprising a DNA sequence encoding a recombinant hepatitis B viral surface antigen protein, wherein said plant is capable of synthesizing a recombinant hepatitis B viral surface antigen protein which assembles into antigenic particles.
Claim 3
Independent Claim
77 words
A method for producing an antigenic composition comprising the steps of: constructing a plasmid vector or a DNA fragment by operably linking a DNA sequence encoding a hepatitis B viral surface antigen protein which protein is capable of assembling into antigenic particles, to a tobacco plant-functional promoter capable of directing the expression of said protein--encoding DNA sequence in a tobacco plant; transforming a tobacco plant cell with said plasmid vector or DNA fragment; and, recovering said particles.
Claim 4
Independent Claim
77 words
A method for producing an antigenic composition comprising the steps of: constructing a plasmid vector or a DNA fragment by operably linking a DNA sequence encoding a hepatitis B viral surface antigen protein which protein is capable of assembling into antigenic particles, to a tobacco plant-functional promoter capable of directing the expression of said protein--encoding DNA sequence in a tobacco plant; transforming a tobacco plant cell with said plasmid vector or DNA fragment; and, recovering said particles.
Claim 5
Dependent Claim
26 words
The method of claim 2 further comprising the step of; prior to recovering said particles, regenerating a transgenic tobacco plant from said transgenic tobacco plant cell.
Claim 6
Dependent Claim
18 words
The method of claim 2 wherein said recovery step further comprises obtaining an extract of said plant cell.
Claim 7
Dependent Claim
20 words
The method of claim 3 wherein said recovery step further comprises harvesting at least a portion of said transgenic plant.
Claim 8
Dependent Claim
15 words
The method of claim 2 wherein said plant cell is transformed utilizing an Agrobacterium system.
Claim 9
Dependent Claim
15 words
The method of claim 6 wherein said Agrobacterium system is an Agrobacterium tumefaciens-Ti plasmid system.
Claim 10
Dependent Claim
17 words
The method of claim 2 wherein said plant cell is transformed utilizing a microparticle bombardment transformation system.
Claim 11
Dependent Claim
13 words
The method of claim 2 wherein said plasmid vector is a binary vector.
Claim 12
Dependent Claim
13 words
The method of claim 2 wherein said plasmid vector is an integrative vector.
Claim 13
Dependent Claim
11 words
The method of claim 2 wherein said plasmid vector is pB121.
Claim 14
Dependent Claim
13 words
The method of claim 2 wherein said plant cell is transformed by microinjection.
Claim 15
Dependent Claim
16 words
The method of claim 2 wherein said plant cell is transformed by polyethylene glycol mediated uptake.
Claim 16
Dependent Claim
13 words
The method of claim 2 wherein said plant cell is transformed by electroporation.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-03-17 | 2000-09-16 | 2001-03-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-03-17 | 2004-09-16 | 2005-03-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-03-17 | 2008-09-16 | 2009-03-18 | $7700.00 | Window Closed / Lapsed |