US5484719A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Vaccines produced and administered through edible plants
Assignee: TERRAMED Inc
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Filing: 1993-11-23
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Granted: 1996-01-16
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Expires: 2013-11-23
Abstract
Abstract The oral vaccine of the present invention is produced in edible transgenic plants and then administered through the consumption of the edible portion of those plants. A DNA sequence encoding for the expression of a surface antigen of a pathogen is isolated and ligated to a promoter which can regulate the production of the surface antigen in a transgenic plant. This gene is then transferred to plant cells using a procedure that results in its integration into the plant genome, such as through the use of an Agrobacterium tumenfaciens plasmid vector system. Preferably, the foreign gene is expressed in an portion of the plant that is edible by humans or animals. The vaccine is administered through the consumption of the edible plant as food, preferably in the form of a fruit or vegetable juice which can be taken orally.
Inventors
- 👤 Dominic M. Lam
- 👤 Charles J. Arntzen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 14:50
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; A; A61; A61K; A61K39/00; A61K39/12; A61K39/29; A61K39/292; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8241; C12N15/8242; C12N15/8257; C12N15/8258; A61K2039/51; A61K2039/525; A61K2039/5258; A61K2039/54; A61K2039/541; A61K2039/542; C07K2319/00; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122; C12N2730/10134
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
41 words
A plasmid vector for transforming a plant comprising: a DNA sequence encoding a recombinant hepatitis B viral surface antigen protein; and a plant-functional promoter operably linked to said DNA sequence capable of directing the synthesis of said protein in said plant.
Claim 2
Dependent Claim
41 words
A plasmid vector for transforming a plant comprising: a DNA sequence encoding a recombinant hepatitis B viral surface antigen protein; and a plant-functional promoter operably linked to said DNA sequence capable of directing the synthesis of said protein in said plant.
Claim 3
Dependent Claim
14 words
The plasmid vector of claim 1 further including a selectable or scorable marker gene.
Claim 4
Dependent Claim
14 words
The plasmid vector of claim 1 wherein said plant promoter is a CaMV35S promoter.
Claim 5
Dependent Claim
11 words
The plasmid vector of claim 1 wherein said plant is edible.
Claim 6
Independent Claim
56 words
A method for constructing a transgenic tobacco plant cell comprising: constructing a plasmid vector by operably linking a DNA sequence, said sequence encoding a hepatitis B viral surface antigen protein, to a plant-functional promoter capable of directing the synthesis of said protein in said tobacco plant; and transforming a tobacco plant cell with said plasmid vector.
Claim 7
Independent Claim
56 words
A method for constructing a transgenic tobacco plant cell comprising: constructing a plasmid vector by operably linking a DNA sequence, said sequence encoding a hepatitis B viral surface antigen protein, to a plant-functional promoter capable of directing the synthesis of said protein in said tobacco plant; and transforming a tobacco plant cell with said plasmid vector.
Claim 8
Dependent Claim
19 words
The method of claim 5 further comprising the step of regenerating a transgenic plant from said transgenic plant cell.
Claim 9
Dependent Claim
13 words
The method of claim 5 wherein the plasmid vector is a DNA virus.
Claim 10
Dependent Claim
13 words
The method of claim 5 wherein the plasmid vector is a binary vector.
Claim 11
Dependent Claim
13 words
The method of claim 5 wherein the plasmid vector is an integrative vector.
Claim 12
Dependent Claim
11 words
The method of claim 5 wherein the plasmid vector is pB121.
Claim 13
Dependent Claim
13 words
The method of claim 5 wherein the plant cell is transformed by microinjection.
Claim 14
Dependent Claim
16 words
The method of claim 5 wherein the plant cell is transformed by polyethylene glycol mediated uptake.
Claim 15
Dependent Claim
13 words
The method of claim 5 wherein the plant cell is transformed by electroporation.
Claim 16
Dependent Claim
14 words
The method of claim 5 wherein the plant cell is transformed by microparticle bombardment.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-01-15 | 1999-07-17 | 2000-01-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-01-15 | 2003-07-17 | 2004-01-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-01-15 | 2007-07-17 | 2008-01-16 | $7700.00 | Window Closed / Lapsed |