US4940835A
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Glyphosate-resistant plants
Assignee: Monsanto Technology LLC
•
Filing: 1986-07-07
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Granted: 1990-07-10
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Expires: 2006-07-07
Abstract
Abstract This invention involves a cloning or expression vector comprising a gene which encodes 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) polypeptide which, when expressed in a plant cell contains a chloroplast transit peptide which allows the polypeptide, or an enzymatically active portion thereof, to be transported from the cytoplasm of the plant cell into a chloroplast in the plant cell, and confers a substantial degree of glyphosate resistance upon the plant cell and plants regenerated therefrom. The EPSPS coding sequence may be ligated to a strong promoter, such as the 35S promoter from cauliflower mosaic virus, to create a chimeric gene. Such genes can be inserted into plant transformation vectors, and subsequently introduced into plant cells. Plant cells transformed using such genes and plants regenerated therefrom have been shown to exhibit a substantial degree of glyphosate resistance.
Inventors
- 👤 Dilip M. Shah
- 👤 Stephen G. Rogers
- 👤 Robert B. Horsch
- 👤 Robert T. Fraley
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 04:56
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8241; C12N15/8261; C12N15/8271; C12N15/8274; C12N15/8275
Claims Analysis
Total Claims: 61
Claim 1
Independent Claim
114 words
A chimeric plant gene which comprises: (a) a promoter sequence which functions in plant cells; (b) a coding sequence which causes the production of RNA, encoding a chloroplast transit peptide/5-enolpyruvylshikimate-3-phosphate synthase fusion polypeptide, which chloroplast transit peptide permits the fusion polypeptide to be imported into a chloroplast of a plant cell; and (c) a 3' non-translated region which encodes a polyadenylation signal which functions in plant cells to cause the addition of polyadenylate nucleotides to the 3' end of the RNA; the promoter being heterologous with respect to the coding sequence and adapted to cause sufficient expression of the fusion polypeptide to enhance the glyphosate resistance of a plant cell transformed with the gene.
Claim 2
Dependent Claim
114 words
A chimeric plant gene which comprises: (a) a promoter sequence which functions in plant cells; (b) a coding sequence which causes the production of RNA, encoding a chloroplast transit peptide/5-enolpyruvylshikimate-3-phosphate synthase fusion polypeptide, which chloroplast transit peptide permits the fusion polypeptide to be imported into a chloroplast of a plant cell; and (c) a 3' non-translated region which encodes a polyadenylation signal which functions in plant cells to cause the addition of polyadenylate nucleotides to the 3' end of the RNA; the promoter being heterologous with respect to the coding sequence and adapted to cause sufficient expression of the fusion polypeptide to enhance the glyphosate resistance of a plant cell transformed with the gene.
Claim 3
Dependent Claim
17 words
A chimeric gene of claim 1 in which the promoter sequence is a plant virus promoter sequence.
Claim 4
Dependent Claim
20 words
A chimeric gene of claim 2 in which the promoter sequence is a promoter sequence from cauliflower mosaic virus (CaMV).
Claim 5
Dependent Claim
16 words
A chimeric gene of claim 3 in which the promoter sequence is the CaMV35S promoter sequence.
Claim 6
Dependent Claim
17 words
A chimeric gene of claim 1 in which the coding sequence encodes a mutant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS).
Claim 7
Dependent Claim
28 words
A chimeric gene of claim 1 in which the EPSPS coding sequence encodes an EPSPS from an organism selected from the group consisting of bacteria, fungi and plants.
Claim 8
Dependent Claim
18 words
A chimeric gene of claim 1 in which the chloroplast transit peptide is from a plant EPSPS gene.
Claim 9
Dependent Claim
13 words
A cloning or expression vector comprising a chimeric plant gene of claim 1.
Claim 10
Dependent Claim
24 words
A cloning or expression vector of claim 8 in which the chimeric plant gene encodes a chloroplast transit peptide of a plant EPSPS gene.
Claim 11
Dependent Claim
22 words
A cloning or expression vector of claim 9 in which the chimeric plant gene comprises a promoter sequence from a plant virus.
Claim 12
Dependent Claim
22 words
A cloning or expression vector of claim 10 in which the promoter sequence is a promoter sequence from cauliflower mosaic virus (CaMV).
Claim 13
Dependent Claim
18 words
A cloning or expression vector of claim 11 in which the promoter sequence is the CaMV35S promoter sequence.
Claim 14
Dependent Claim
23 words
A cloning or expression vector of claim 8 in which the chimeric plant gene comprises a coding sequence encoding a mutant 5-enolpyruvylshikimate-3-phosphate synthase.
Claim 15
Dependent Claim
29 words
A cloning or expression vector of claim 8 in which the coding sequence encodes an EPSPS from an organism selected from the group consisting of bacteria, fungi and plants.
Claim 16
Dependent Claim
12 words
A plant transformation vector which comprises a chimeric gene of claim 1.
Claim 17
Dependent Claim
23 words
A plant transformation vector of claim 15 in which the chimeric plant gene encodes a chloroplast transit peptide of a plant EPSPS gene.
Claim 18
Dependent Claim
21 words
A plant transformation vector of claim 15 in which the chimeric plant gene comprises a promoter sequence from a plant virus.
Claim 19
Dependent Claim
21 words
A plant transformation vector of claim 17 in which the promoter sequence is a promoter sequence from cauliflower mosaic virus (CaMV).
Claim 20
Dependent Claim
17 words
A plant transformation vector of claim 18 in which the promoter sequence is the CaMV35S promoter sequence.
Claim 21
Dependent Claim
22 words
A plant transformation vector of claim 15 in which the chimeric plant gene comprises a coding sequence encoding a mutant 5-enolpyruvylshikimate-3-phosphate synthase.
Claim 22
Dependent Claim
28 words
A plant transformation vector of claim 15 in which the coding sequence encodes an EPSPS from an organism selected from the group consisting of bacteria, fungi and plants.
Claim 23
Dependent Claim
12 words
A glyphosate-resistant plant cell comprising a chimeric plant gene of claim 1.
Claim 24
Dependent Claim
18 words
A glyphosate-resistant plant cell of claim 22 in which the promoter sequence is a plant virus promoter sequence.
Claim 25
Dependent Claim
21 words
A glyphosate-resistant plant cell of claim 23 in which the promoter sequence is a promoter sequence from cauliflower mosaic virus (CaMv).
Claim 26
Dependent Claim
17 words
A glyphosate-resistant plant cell of claim 24 in which the promoter sequence is the CaMV35S promoter sequence.
Claim 27
Dependent Claim
17 words
A glyphosate-resistant plant cell of claim 22 in which the coding sequence encodes a mutant 5-enolpyruvylshikimate-3-phosphate synthase.
Claim 28
Dependent Claim
28 words
A glyphosate-resistant plant cell of claim 22 in which the coding sequence encodes a EPSPS from an organism selected from the group consisting of bacteria, fungi and plants.
Claim 29
Dependent Claim
19 words
A glyphosate-resistant plant cell of claim 22 in which the chloroplast transit peptide is from a plant EPSPS gene.
Claim 30
Dependent Claim
21 words
A glyphosate-resistant dicotyledonous plant which has been regenerated from a glyphosate-resistant plant cell comprising the chimeric plant gene of claim 1.
Claim 31
Dependent Claim
17 words
A glyphosate-resistant plant of claim 29 in which the promoter sequence is a plant virus promoter sequence.
Claim 32
Dependent Claim
20 words
A glyphosate-resistant plant of claim 30 in which the promoter sequence is a promoter sequence from cauliflower mosaic virus (CaMV).
Claim 33
Dependent Claim
16 words
A glyphosate-resistant plant of claim 31 in which the promoter sequence is the CaMv35S promoter sequence.
Claim 34
Dependent Claim
21 words
A glyphosate-resistant plant of claim 29 in which the chimeric plant gene comprises a coding sequence encoding a mutant 5-enolpyruvylshikimate-3-phosphate synthase.
Claim 35
Dependent Claim
27 words
A glyphosate-resistant plant of claim 29 in which the coding sequence encodes an EPSPS from an organism selected from the group consisting of bacteria, fungi and plants.
Claim 36
Dependent Claim
19 words
A glyphosate-resistant plant cell of claim 29 in which the chloroplast transit peptide is from a plant EPSPS gene.
Claim 37
Dependent Claim
37 words
A method for producing a glyphosate-resistant dicotyledonous plant which comprises (a) transforming plant cells using an Agrobacterium transformation vector comprising a chimeric plant gene of claim 1; and (b) regenerating glyphosate-resistant plants from said transformed plant cells.
Claim 38
Dependent Claim
17 words
A method of claim 36 in which the chimeric plant gene comprises a plant virus promoter sequence.
Claim 39
Dependent Claim
19 words
A method of claim 37 in which the promoter sequence is a promoter sequence from cauliflower mosaic virus (CaMV).
Claim 40
Dependent Claim
15 words
A method of claim 38 in which the promoter sequence is the CaMV35S promoter sequence.
Claim 41
Dependent Claim
19 words
A method of claim 36 in which the chimeric gene comprises a coding sequence encoding a mutant 5-enolpyruvylshikimate-3-phosphate synthase.
Claim 42
Dependent Claim
26 words
A method of claim 36 in which the coding sequence encodes an EPSPS from an organism selected from the group consisting of bacteria, fungi and plants.
Claim 43
Dependent Claim
20 words
A method of claim 36 in which the coding sequence encodes the chloroplast transit peptide from a plant EPSPS gene.
Claim 44
Dependent Claim
22 words
A method for producing a glyphosate-resistant plant cell which comprises transforming the plant cell with a plant transformation vector of claim 15.
Claim 45
Dependent Claim
18 words
A method of claim 43 in which the chimeric gene comprises a promoter sequence from a plant virus.
Claim 46
Dependent Claim
19 words
A method of claim 44 in which the promoter sequence is a promoter sequence from cauliflower mosaic virus (CaMV).
Claim 47
Dependent Claim
15 words
A method of claim 45 in which the promoter sequence is the CaMV35S promoter sequence.
Claim 48
Dependent Claim
19 words
A method of claim 43 in which the chimeric gene comprises a coding sequence encoding a mutant 5-enolpyruvylshikimate-3-phosphate synthase.
Claim 49
Dependent Claim
26 words
A method of claim 43 in which the coding sequence encodes an EPSPS from an organism selected from the group consisting of bacteria, fungi and plants.
Claim 50
Dependent Claim
20 words
A method of claim 43 in which the coding sequence encodes the chloroplast transit peptide from a plant EPSPS gene.
Claim 51
Dependent Claim
7 words
A glyphosate-resistant tomato cell of claim 22.
Claim 52
Dependent Claim
7 words
A glyphosate-resistant tobacco cell of claim 22.
Claim 53
Dependent Claim
9 words
A glyphosate-resistant oil seed rape cell of claim 22.
Claim 54
Dependent Claim
7 words
A glyphosate-resistant flax cell of claim 22.
Claim 55
Dependent Claim
7 words
A glyphosate-resistant soybean cell of claim 22.
Claim 56
Dependent Claim
7 words
A glyphosate-resistant sunflower cell of claim 22.
Claim 57
Dependent Claim
8 words
A glyphosate-resistant sugar beet cell of claim 54.
Claim 58
Dependent Claim
7 words
A glyphosate-resistant alfalfa cell of claim 22.
Claim 59
Dependent Claim
7 words
A glyphosate-resistant cotton cell of claim 22.
Claim 60
Dependent Claim
6 words
Plasmid pMON546, ATCC accession number 53213.
Claim 61
Dependent Claim
6 words
Plasmid pMON546, ATCC accession number 53213.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-07-09 | 1994-01-08 | 1994-07-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-07-09 | 1998-01-08 | 1998-07-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-07-09 | 2002-01-08 | 2002-07-10 | $7700.00 | Window Closed / Lapsed |