EP0115673A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Inhibition resistant 5-enolpyruvyl-3-phoshoshikimate synthetase, production and use
Assignee: Monsanto Co
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Filing: 1983-11-11
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Granted: —
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Expires: 2003-11-11
Abstract
Abstract Enhanced resistance to glyphosate, an inhibitor of the aromatic amino acid biosynthesis pathway, is imparted to a glyphosate sensitive host A mutated aroA gene is employed which expresses 5-enolpyruvyl-3-phosphoshikimate synthetase (EC: 2.5.1.19) (ES-3-P synthetase). Methods are provided for obtaining the aroA mutation which provides the enzyme resistant to inhibition by glyphosate, means for introducing the structural gene into a sensitive host, as well as providing a method of producing the enzyme.The E. coli strain C600(pPMG1) has been deposited at the A.T.C.C. on Dec. 14, 1982 and been given A.T.C.C. accession no. 39 256.
Inventors
- 👤 Luca Comai
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 05:07
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/10; C12N9/1085; C12N9/1092; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8241; C12N15/8261; C12N15/8271; C12N15/8274; C12N15/8275; C12Q; C12Q1/00; C12Q1/48; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/581
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
28 words
A cell containing glyphosate resistant 5-enolpyruvyl-3-phosphoshikimate synthetase having at least one mutation originating from in vitro mutation in the arc locus for the structural gene expressing said synthetase.
Claim 2
Dependent Claim
28 words
A cell containing glyphosate resistant 5-enolpyruvyl-3-phosphoshikimate synthetase having at least one mutation originating from in vitro mutation in the arc locus for the structural gene expressing said synthetase.
Claim 3
Dependent Claim
15 words
A method according to Claim 1, wherein said structural gene is on an episomal element.
Claim 4
Dependent Claim
11 words
A method according to Claim 2, wherein said cell is prokaryotic.
Claim 5
Dependent Claim
19 words
A DNA sequence of less than about 5Kb having a structural gene coding for a glyphosate resistant 5-enolpyruvyl-3-phosphoshikimate synthetase.
Claim 6
Dependent Claim
19 words
A DNA sequence of less than about 5Kb having a structural gene coding for a glyphosate resistant 5-enolpyruvyl-3-phosphoshikimate synthetase.
Claim 7
Dependent Claim
15 words
A DNA sequence according to Claim 4, having a mutation originating from in vitro mutation.
Claim 8
Dependent Claim
15 words
A DNA sequence according to Claim 5, wherein said synthetase is a mutated prokaryotic synthetase.
Claim 9
Dependent Claim
22 words
A DNA construct comprising a DNA sequence according to Claim 4 and a replication system capable of replication in a cellular host.
Claim 10
Dependent Claim
16 words
A DNA construct according to Claim 7, wherein said replication system is recognized.by a prokaryotic host.
Claim 11
Dependent Claim
4 words
Glyphosate resistant 5-enolpyruvyl-3-phosphoshikimate synthetase.
Claim 12
Dependent Claim
4 words
Glyphosate resistant 5-enolpyruvyl-3-phosphoshikimate synthetase.
Claim 13
Dependent Claim
9 words
S. typhimurium strain CT7, aroA + Pmg r .
Claim 14
Dependent Claim
9 words
S. typhimurium strain CT7, aroA + Pmg r .
Claim 15
Dependent Claim
32 words
A method for producing glyphosate resistant 5-enolpyruvyl-3-phosphshikimate synthetase which comprises: growing said S. typhimurium according to Claim 10 in an appropriate nutrient medium; and lysing said S. typhimurium and isolating said synthetase.
Claim 16
Independent Claim
92 words
A method for obtaining a mutated enzyme having a selectable property which comprises: mutating in vitro a cellular host having a structural gene expressing said enzyme and selecting for mutants having said selectable property; cleaving the genome of said mutants to produce fragments of a desired size range; cloning said fragments in an auxotrophic host wherein the mutated structural gene transforms said host to prototrophy and selecting for prototrophic hosts having said selectable property; and isolating the DNA sequence having the mutated structural gene expressing said mutated enzyme having said selectable property.
Claim 17
Independent Claim
92 words
A method for obtaining a mutated enzyme having a selectable property which comprises: mutating in vitro a cellular host having a structural gene expressing said enzyme and selecting for mutants having said selectable property; cleaving the genome of said mutants to produce fragments of a desired size range; cloning said fragments in an auxotrophic host wherein the mutated structural gene transforms said host to prototrophy and selecting for prototrophic hosts having said selectable property; and isolating the DNA sequence having the mutated structural gene expressing said mutated enzyme having said selectable property.
References Cited (Backward Citations)
No citation records indexed.
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