US5605838A
Expired / Lapsed
US
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Method for the quantitative analysis of sample liquid
Assignee: Roche Diagnostics GmbH
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Filing: 1994-09-19
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Granted: 1997-02-25
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Expires: 2014-09-19
Abstract
Abstract The invention concerns a method for the quantitative analysis of sample liquids. A sample is dried and irradiated with visible and/or infrared light. Light that is diffusely or specularly reflected from the sample and sample carrier is detected and analysed. Furthermore the invention concerns a system for carrying out the method according to the invention and a sample carrier having a diffusely or specularly reflecting surface.
Inventors
- 👤 Juergen Backhaus
- 👤 Reinhold Mischler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 22:34
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/508; B01L3/5085; G; G01; G01N; G01N21/00; G01N21/17; G01N21/25; G01N21/31; G01N21/35; G01N21/3563; G01N21/3577; G01N21/47; G01N1/00; G01N1/28; G01N1/40; G01N1/4022; G01N2001/4027; G01N2201/00; G01N2201/12; G01N2201/129; Y; Y10; Y10T; Y10T436/00; Y10T436/11; Y10T436/112499; Y10T436/25; Y10T436/25375
Claims Analysis
Total Claims: 30
Claim 1
Independent Claim
130 words
A method for the quantitative analysis of sample liquids, comprising the steps of: applying a defined amount of sample liquid containing a solvent and at least one component to be analyzed on the surface of a sample carrier, drying the sample liquid to produce a residue of drying having a solvent content below 20% by weight, irradiating the residue of drying with a cone of rays of radiation in at least one of the infrared or visible range of the spectrum, wherein the residue of drying is located completely in the cone of rays, detecting radiation which has been diffusely reflected from the residue of drying and the sample carrier, and analyzing the detected radiation in order to calculate the concentration of at least one constituent of the sample liquid.
Claim 2
Independent Claim
130 words
A method for the quantitative analysis of sample liquids, comprising the steps of: applying a defined amount of sample liquid containing a solvent and at least one component to be analyzed on the surface of a sample carrier, drying the sample liquid to produce a residue of drying having a solvent content below 20% by weight, irradiating the residue of drying with a cone of rays of radiation in at least one of the infrared or visible range of the spectrum, wherein the residue of drying is located completely in the cone of rays, detecting radiation which has been diffusely reflected from the residue of drying and the sample carrier, and analyzing the detected radiation in order to calculate the concentration of at least one constituent of the sample liquid.
Claim 3
Dependent Claim
23 words
Method of claim 1, wherein less than 50 μl of sample liquid is applied to the sample carrier surface in a coherent form.
Claim 4
Dependent Claim
16 words
Method of claim 2, wherein the amount of sample liquid is from 0.1 to 10 μl.
Claim 5
Dependent Claim
12 words
Method of claim 1, wherein the sample liquid is a body fluid.
Claim 6
Dependent Claim
17 words
Method of claim 1, wherein the radiation has wavelengths in the range of 0.3 to 25 μm.
Claim 7
Dependent Claim
17 words
Method of claim 5, wherein the radiation wavelengths are in the range of 2.5 to 25 μm.
Claim 8
Dependent Claim
21 words
Method of claim 1, wherein the sample liquid cannot penetrate into a substantial portion of the surface of the sample carrier.
Claim 9
Dependent Claim
23 words
Method of claim 1, further comprising coating the surface of said sample carrier with reagents suitable for the determination of repeated kinetic measurements.
Claim 10
Dependent Claim
31 words
Method of claim 1, wherein the sample liquid is applied to the sample carrier surface by first applying an amount of sample, and thereafter removing a part of the sample amount.
Claim 11
Dependent Claim
16 words
Method of claim 9, wherein the part of the sample amount removed is greater than 50%.
Claim 12
Dependent Claim
30 words
Method of claim 9, wherein the steps of the application of the liquid sample and the removal of part of the liquid sample are carried out using a pipetting means.
Claim 13
Dependent Claim
54 words
Method of claim 1, wherein the sample liquid is applied to the sample carrier surface by taking up the sample on an absorbent stamp, thereafter pressing the stamp onto the surface of the sample carrier and lifting the stamp from the surface to leave the amount of sample liquid on the sample carrier surface.
Claim 14
Dependent Claim
29 words
Method of claim 1 wherein detergents, proteins or macromolecular substances are added to the sample liquid in order to change the drying and/or film-forming properties of the sample liquid.
Claim 15
Independent Claim
130 words
A method for the quantitative analysis of sample liquids, comprising the steps of: applying a defined amount of sample liquid containing a solvent and at least one component to be analyzed on the surface of a sample carrier, drying the sample liquid to produce a residue of drying having a solvent content below 20% by weight, irradiating the residue of drying with a cone of rays of radiation in at least one of the infrared or visible range of the spectrum, wherein the residue of drying is located completely in the cone of rays, detecting radiation which has been specularly reflected from the residue of drying and the sample carrier, and analyzing the detected radiation in order to calculate the concentration of at least one constituent of the sample liquid.
Claim 16
Independent Claim
130 words
A method for the quantitative analysis of sample liquids, comprising the steps of: applying a defined amount of sample liquid containing a solvent and at least one component to be analyzed on the surface of a sample carrier, drying the sample liquid to produce a residue of drying having a solvent content below 20% by weight, irradiating the residue of drying with a cone of rays of radiation in at least one of the infrared or visible range of the spectrum, wherein the residue of drying is located completely in the cone of rays, detecting radiation which has been specularly reflected from the residue of drying and the sample carrier, and analyzing the detected radiation in order to calculate the concentration of at least one constituent of the sample liquid.
Claim 17
Dependent Claim
23 words
Method of claim 14, wherein less than 50 μ1 of sample liquid is applied to the sample carrier surface in a coherent form.
Claim 18
Dependent Claim
16 words
Method of claim 15, wherein the amount of sample liquid is from 0.1 to 10 μl.
Claim 19
Dependent Claim
12 words
Method of claim 14, wherein the sample liquid is a body fluid.
Claim 20
Dependent Claim
17 words
Method of claim 14, wherein the radiation has wavelengths in the range of 0.3 to 25 μm.
Claim 21
Dependent Claim
17 words
Method of claim 18, wherein the radiation wavelengths are in the range of 2.5 to 25 μm.
Claim 22
Dependent Claim
21 words
Method of claim 14, wherein the sample liquid cannot penetrate into a substantial portion of the surface of the sample carrier.
Claim 23
Dependent Claim
23 words
Method of claim 14, further comprising coating the surface of said sample carrier with reagents suitable for the determination of repeated kinetic measurements.
Claim 24
Dependent Claim
31 words
Method of claim 14, wherein the sample liquid is applied to the sample carrier surface by first applying an amount of sample, and thereafter removing a part of the sample amount.
Claim 25
Dependent Claim
16 words
Method of claim 22, wherein the part of the sample amount removed is greater than 50%.
Claim 26
Dependent Claim
30 words
Method of claim 22, wherein the steps of the application of the liquid sample and the removal of part of the liquid sample are carried out using a pipetting means.
Claim 27
Dependent Claim
56 words
Method of claim 14, wherein the sample liquid is applied to the sample carrier surface by taking up the sample on an absorbent stamp, thereafter a pressing the stamp onto the surface of the sample carrier and a lifting the stamp from the surface to leave the amount of sample. liquid on the sample carrier surface.
Claim 28
Dependent Claim
30 words
Method of claim 14, wherein detergents, proteins or macromolecular substances are added to the sample liquid in order to change the drying and/or film forming properties of the sample liquid.
Claim 29
Dependent Claim
19 words
Method of claim 14, wherein the sample carrier has an average depth of roughness of less than 200 μm.
Claim 30
Dependent Claim
19 words
Method of claim 1, wherein the sample carrier has an average depth of roughness of less than 200 μm.
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