Patent US5595908A successfully refreshed from Google Patents.
US5595908A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Piezoelectric device for detection of polynucleotide hybridization
Assignee: University of Southern Mississippi
•
Filing: 1989-08-11
•
Granted: 1997-01-21
•
Expires: 2009-08-11
Abstract
Abstract A system for detecting polynucleotide hybridization is disclosed. A polynucleotide is immobilized on a surface of a piezoelectric crystal. The resonance frequency of the piezoelectric crystal is measured through means for determining the resonant frequency of a piezoelectric crystal. A separate source of polynucleotide is exposed to the polynucleotide-coated piezoelectric crystal for a sufficient length of time and under conditions suitable for hybridization. The resonance frequency of the crystal is then again measured, and the difference between the resonance frequency before and after the incubation period will indicate the extent of hybridization.
Inventors
- 👤 Newton C. Fawcett
- 👤 Jeffrey A. Evans
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 18:22
CPC Classifications
G; G01; G01N; G01N29/00; G01N29/02; G01N29/036; C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/6825; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/542; G01N33/543; G01N33/54366; G01N33/54373; G01N2291/00; G01N2291/02; G01N2291/025; G01N2291/0256; G01N2291/0257
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
36 words
A device for detecting polynucleotide hybridization comprising: (a) a piezoelectric crystal; (b) a polynucleotide attached to a surface of said crystal by a linker; and (c) a means for determining the resonance frequency of said crystal.
Claim 2
Independent Claim
36 words
A device for detecting polynucleotide hybridization comprising: (a) a piezoelectric crystal; (b) a polynucleotide attached to a surface of said crystal by a linker; and (c) a means for determining the resonance frequency of said crystal.
Claim 3
Dependent Claim
24 words
The device of claim 1 wherein said linker is selected from the group comprising organic compounds characterized by the presence of an azido group.
Claim 4
Dependent Claim
16 words
The device of claim 1 wherein said linker is selected from 2,6-bis(4-azidobenzylidene) cyclohexanone and triazido trinitrobenzene.
Claim 5
Independent Claim
65 words
A device for detecting polynucleotide hybridization comprising: (a) a piezoelectric crystal; (b) a polynucleotide attached to a surface of said crystal by a polymer coated on at least a portion of said crystal, said polymer being selected from a class of polymers characterized by hydrolyrically stable, hydrophobic backbones substituted with reactive pendant groups; and (c) a means for determining the resonance frequency of said crystal.
Claim 6
Independent Claim
65 words
A device for detecting polynucleotide hybridization comprising: (a) a piezoelectric crystal; (b) a polynucleotide attached to a surface of said crystal by a polymer coated on at least a portion of said crystal, said polymer being selected from a class of polymers characterized by hydrolyrically stable, hydrophobic backbones substituted with reactive pendant groups; and (c) a means for determining the resonance frequency of said crystal.
Claim 7
Dependent Claim
21 words
The device of claim 4 wherein said polymer is selected from the group of polymers consisting of poly(butyl methacrylate) and polyurethane.
Claim 8
Dependent Claim
12 words
The device of claim 4 wherein said polymer is a synthetic polynucleotide.
Claim 9
Dependent Claim
49 words
The device of claim 4 wherein said polymer is selected from the group consisting of the copolymers of styrene and p-x-methylstyrene, ethylene and N-(6-x-hexyl)-acrylamide, propylene and N-(6-x-hexy)-acrylamide, alkylated siloxanes and 6-x-hexyl siloxanes and alkyl-substituted siloxanes, where X is a reactive group capable of combining with polynucleotides or derivatized polynucleotides.
Claim 10
Independent Claim
73 words
A device for detecting polynucleotide hybridization comprising: (a) a piezoelectric crystal; (b) a polynucleotide attached to a surface of said crystal by enzymatically ligating said polynucleotide using DNA or RNA ligase to a different polynucleotide in order to then covalently bond said different polynucleotide by chemical reaction to a functional group which is in turn chemically bonded to said crystal surface; and (c) a means for determining the resonance frequency of said crystal.
Claim 11
Independent Claim
73 words
A device for detecting polynucleotide hybridization comprising: (a) a piezoelectric crystal; (b) a polynucleotide attached to a surface of said crystal by enzymatically ligating said polynucleotide using DNA or RNA ligase to a different polynucleotide in order to then covalently bond said different polynucleotide by chemical reaction to a functional group which is in turn chemically bonded to said crystal surface; and (c) a means for determining the resonance frequency of said crystal.
Claim 12
Independent Claim
34 words
A device for detecting polynucleotide hybridization comprising: (a) a piezoelectric crystal; (b) a polynucleotide covalently bonded to a surface of said crystal; and (c) a means for determining the resonance frequency of said crystal.
Claim 13
Independent Claim
34 words
A device for detecting polynucleotide hybridization comprising: (a) a piezoelectric crystal; (b) a polynucleotide covalently bonded to a surface of said crystal; and (c) a means for determining the resonance frequency of said crystal.
References Cited (Backward Citations)
US4242096A
Cited
WO1980002158A1
Cited
WO1980002201A1
Cited
US4236893A
Cited
US4314821A
Cited
US4376110A
Cited
EP0063879A2
Cited
US4486539A
Cited
EP0127438B1
Cited
WO1985002627A1
Cited
US4591550A
Cited
WO1987002066A1
Cited
US4788466A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-01-21 | 2000-07-22 | 2001-01-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-01-21 | 2004-07-22 | 2005-01-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-01-21 | 2008-07-22 | 2009-01-21 | $7700.00 | Window Closed / Lapsed |