EP0127438B1
Expired / Lapsed
EP
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Diagnostic device incorporating a biochemical ligand
Assignee: National Research Development Corp UK
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Filing: 1984-05-23
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Granted: 1987-12-16
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Expires: 2004-05-23
Abstract
No abstract record available.
Inventors
- 👤 Christopher Robin Lowe
- 👤 Fergus Gerard Paul Earley
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-02 07:06
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/002; C12Q1/003; C07; C07B; C07B2200/00; C07B2200/11; G01N27/00; G01N27/26; G01N27/403; G01N27/414; G01N27/4145; Y; Y10; Y10S; Y10S436/00; Y10S436/806
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
75 words
A device useful in diagnostics, which comprises a sensor having a surface to which a group comprising a residue of a biochemical ligand is attached covalently, whereby a physical characteristic of the sensor varies according to whether a binding partner is bound to the ligand, characteised in that the biochemical ligand residue of the group is attached covalently to the surface of the sensor in selected areas only thereof and through a photoactivated covalent linkage.
Claim 2
Independent Claim
75 words
A device useful in diagnostics, which comprises a sensor having a surface to which a group comprising a residue of a biochemical ligand is attached covalently, whereby a physical characteristic of the sensor varies according to whether a binding partner is bound to the ligand, characteised in that the biochemical ligand residue of the group is attached covalently to the surface of the sensor in selected areas only thereof and through a photoactivated covalent linkage.
Claim 3
Dependent Claim
37 words
A device according to Claim 1, wherein the biochemical ligand is an antigen or hapten and a physical characteristic of the sensor varies according to whether or not an antibody is bound to the antigen or hapten.
Claim 4
Dependent Claim
35 words
A device according to Claim 1, wherein the biochemical ligand is an antibody and a physical characteristic of the sensor varies according to whether or not an antigen or hapten is bound to the antibody.
Claim 5
Dependent Claim
33 words
A device according to Claim 1, wherein the biochemical ligand is an enzyme and a physical characteristic of the sensor varies according to whether or not a substrate is bound to the enzyme.
Claim 6
Dependent Claim
23 words
A device according to Claim 1, wherein the biochemical ligand is a co-enzyme, steroid, sugar, electron mediator or other low molecular weight biochemical.
Claim 7
Independent Claim
21 words
A device according to any preceeding claim, wherein the surface of the sensor is of an inorganic oxide, oxynitride or nitride.
Claim 8
Dependent Claim
15 words
A device according to Claim 6, wherein the surface is of silica or silicon nitride.
Claim 9
Dependent Claim
25 words
A device according to Claim 6, wherein the surface is of iron (III) oxide and the sensor is a device for measuring magnetic field strength.
Claim 10
Dependent Claim
23 words
A device according to Claim 6, wherein the surface is of silica or silicon nitride and the sensor is a field effect transistor.
Claim 11
Independent Claim
21 words
A device according to any preceding claim, wherein the photoactivated linkage is provided by the photolysis of an aryl azide group.
Claim 12
Dependent Claim
65 words
A device according to Claim 10, wherein the surface is of silica and the biochemical ligand residue is attached to the surface through a group of formula the left-hand end of which is attached to a silicon atom of the surface and the right-hand end of which is attached to the residue of the biochemical ligand, and n is a number from 3 to 12.
Claim 13
Dependent Claim
70 words
A process of preparing a device claimed in Claim 1, which process comprises modifying the surface of the sensor by attaching to it by covalent bonding a group containing a photoactivatable function, exposing the thus modified surface to photoactivating radiation in selected areas only of the surfaace to activate the function and reacting the function thus activated to bond covalently with a compound providing a residue of the biochemical ligand.
Claim 14
Dependent Claim
15 words
A process according to Claim 12, wherein the modified surface is exposed through a mask.
Claim 15
Dependent Claim
23 words
A process according to Claim 12 or 13, wherein residues of different biochemical ligands are attached to different areas of the same surface.
Claim 16
Dependent Claim
32 words
A process according to Claim 12, 13 or 14, wherein the sensor is a field effect transistor, the surface is of silicon nitride and the photoactivatable function is an aryl azide group.
Claim 17
Dependent Claim
22 words
Use of a device according to any one of Claims 1 to 11 for assay of a binding partner of the ligand.
Claim 18
Dependent Claim
34 words
A kit useful in diagnostics comprising a device claimed in any one of Claims 1 to 11 and at least one binding partner for the biochemical ligand and testing or standardisation of the device.
References Cited (Backward Citations)
No citation records indexed.
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