US5593852A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Subcutaneous glucose electrode
Assignee: Abbott Diabetes Care Inc
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Filing: 1994-09-01
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Granted: 1997-01-14
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Expires: 2014-09-01
Abstract
Abstract A small diameter flexible electrode designed for subcutaneous in vivo amperometric monitoring of glucose is described. The electrode is designed to allow "one-point" in vivo calibration, i.e., to have zero output current at zero glucose concentration, even in the presence of other electroreactive species of serum or blood. The electrode is preferably three or four-layered, with the layers serially deposited within a recess upon the tip of a polyamide insulated gold wire. A first glucose concentration-to-current transducing layer is overcoated with an electrically insulating and glucose flux limiting layer (second layer) on which, optionally, an immobilized interference-eliminating horseradish peroxidase based film is deposited (third layer). An outer (fourth) layer is biocompatible.
Inventors
- 👤 Adam Heller
- 👤 Michael V. Pishko
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 23:57
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54; A; A61; A61B; A61B5/00; A61B5/145; A61B5/14532; A61B5/1468; A61B5/1486; A61B5/1495; C12Q1/001; C12Q1/005; C12Q1/006; Y; Y10; Y10S; Y10S435/00; Y10S435/817; Y10S435/8215; Y10S435/911; Y10S435/913; Y10S435/917; Y10S435/962
Claims Analysis
Total Claims: 35
Claim 1
Independent Claim
62 words
A biosensor for measuring the concentration of a biochemical, comprising: a non-corroding metal or carbon electrode; a sensing layer comprising a redox polymer and a redox enzyme, adjacent to and in electrical contact with the electrode; and a biocompatible layer overcoating the sensing layer, comprising a polymer and not less than 20% water by weight when in equilibrium with physiological body fluids.
Claim 2
Dependent Claim
62 words
A biosensor for measuring the concentration of a biochemical, comprising: a non-corroding metal or carbon electrode; a sensing layer comprising a redox polymer and a redox enzyme, adjacent to and in electrical contact with the electrode; and a biocompatible layer overcoating the sensing layer, comprising a polymer and not less than 20% water by weight when in equilibrium with physiological body fluids.
Claim 3
Dependent Claim
15 words
The biosensor of claim 1, wherein the biocompatible layer is formed of cross-linked poly(ethylene oxide).
Claim 4
Dependent Claim
40 words
The biosensor of claim 1 wherein, said redox polymer is derived from poly(1-vinylimidazole) or a copolymer of (1-vinyl imidazole) bound to a metal ion selected from the group consisting of Os 3+/2+ , Ru 3+/2+ , and Fe 3+/2+ .
Claim 5
Dependent Claim
41 words
The biosensor of claim 1 wherein, the redox potential of said redox polymer is not more reducing than about -0.15 V and not more oxidizing than about +0.15 V versus the standard calomel electrode in an aqueous solution at about pH7.4.
Claim 6
Dependent Claim
12 words
The biosensor of claim 1 further comprising a biochemical flux limiting layer.
Claim 7
Dependent Claim
58 words
The biosensor of claim 1, wherein said electrode is a wire, the wire substantially coated with an electrically insulating polymer and having a gap or recess in the coating exposing a bare, non-insulated surface of the wire, the insulating polymer containing less than 5% water by weight when in equilibrium with physiological body fluids at about 37° C.
Claim 8
Dependent Claim
24 words
The biosensor of claim 6, wherein said exposed wire is recessed in the insulating polymer coat forming a channel in the insulating polymer coat.
Claim 9
Dependent Claim
20 words
The biosensor of claim 6, wherein said channel has a depth ranging from about 20 microns to about 1 mm.
Claim 10
Dependent Claim
86 words
A method for manufacturing the biosensor of claim 8, wherein said recessed channel is formed by electrolytic dissolution of part of the electrically conducting wire, with an oxidizing electrical potential of not less than 0.3 V versus the standard calomel electrode being maintained on the dissolving wire, said wire being immersed in an aqueous solution containing at least one anion selected from the group consisting of CN - , Cl - , Br - and I - at a concentration of at least approximately 0.1 M.
Claim 11
Dependent Claim
46 words
A method for measuring the concentration of a biochemical in an animal comprising: contacting body fluid of the animal with the biosensor of claim 1 to generate an electrical signal; and determining from the generated electrical signal the concentration of a biochemical in the body fluid.
Claim 12
Dependent Claim
14 words
The method of claim 10, wherein said body fluid is blood, plasma or serum.
Claim 13
Dependent Claim
10 words
The method of claim 10, wherein the measuring is intermittent.
Claim 14
Dependent Claim
16 words
The method of claim 10, wherein said contacting is implanting the electrode subcutaneously in said animal.
Claim 15
Dependent Claim
25 words
The method of claim 10, further comprising placing a reference electrode or combined reference and counter electrode on or in the skin of the animal.
Claim 16
Dependent Claim
13 words
The method of claim 10, wherein the biochemical to be detected is glucose.
Claim 17
Dependent Claim
13 words
The method of claim 10 wherein the biochemical to be detected is lactate.
Claim 18
Dependent Claim
60 words
A method of calibrating the biosensor of claim 1, comprising the steps of: independently analyzing the concentration of a biochemical to be measured by the electrode of claim 1 in a single sample of fluid withdrawn from an animal, and relating the electrical signal generated by the electrode to the concentration of biochemical in the single sample of withdrawn fluid.
Claim 19
Dependent Claim
124 words
A biosensor for detecting a biochemical comprising: two or more non-corroding metal or carbon biochemical-responsive electrodes; an electrically insulating polymer coating at least a part of each electrode; a sensing layer adjacent to and in electrical contact with a part of each metal or carbon electrode that is not coated by the insulating polymer, the sensing layer comprising a redox enzyme and redox couple, neither of which is leached by a physiological buffer solution consisting of water, sodium chloride and sodium phosphates at a pH between 6.8 and 7.6 or by serum at about 37° C., wherein the sensing layer generates an electrical signal in the presence of the biochemical; and, a biocompatible polymer coating on an outer surface of each of the electrodes.
Claim 20
Dependent Claim
124 words
A biosensor for detecting a biochemical comprising: two or more non-corroding metal or carbon biochemical-responsive electrodes; an electrically insulating polymer coating at least a part of each electrode; a sensing layer adjacent to and in electrical contact with a part of each metal or carbon electrode that is not coated by the insulating polymer, the sensing layer comprising a redox enzyme and redox couple, neither of which is leached by a physiological buffer solution consisting of water, sodium chloride and sodium phosphates at a pH between 6.8 and 7.6 or by serum at about 37° C., wherein the sensing layer generates an electrical signal in the presence of the biochemical; and, a biocompatible polymer coating on an outer surface of each of the electrodes.
Claim 21
Dependent Claim
23 words
The biosensor of claim 18, wherein said electrically insulating polymer is selected from the group consisting of polyimide, polyester, polyurethane and perfluorinated polymer.
Claim 22
Dependent Claim
17 words
The biosensor of claim 18, wherein the outer diameter of each electrode is smaller than 0.3 mm.
Claim 23
Dependent Claim
21 words
The biosensor of claim 18, wherein each of the biochemical-responsive electrodes further comprises an interference eliminating layer comprising a peroxidase enzyme.
Claim 24
Dependent Claim
49 words
A method for measuring the concentration of a biochemical in an animal comprising: contacting body fluid of an animal with said two or more biochemical-responsive electrodes of claim 18 to generate electrical signals; and determining from the generated electrical signals the concentration of a biochemical in the body fluid.
Claim 25
Dependent Claim
16 words
The method of claim 22, wherein said contacting is implanting the electrodes subcutaneously in said animal.
Claim 26
Dependent Claim
25 words
The method of claim 22 further comprising placing a reference electrode or combined reference and counter electrode on or in the skin of the animal.
Claim 27
Dependent Claim
45 words
The method of claim 22 wherein substantially simultaneous readings of said two or more electrodes are accepted as correctly measuring the concentration of the biochemical when the two or more readings do not differ by more than a specified percentage of the measured electrical signals.
Claim 28
Dependent Claim
40 words
The method of claim 22, wherein readings of said two or more electrodes that do not differ by more than about 20% or by more than about one standard deviation are accepted as correctly measuring the concentration of the biochemical.
Claim 29
Dependent Claim
12 words
The method of claim 22, wherein the biochemical is glucose or lactate.
Claim 30
Dependent Claim
63 words
A method of calibrating the biosensor of claim 18, comprising the steps of: independently analyzing the concentration of a biochemical to be measured by the electrodes of claim 18 in a single sample of fluid withdrawn from an animal, and relating the electrical signal generated by said two or more electrodes to the concentration of biochemical in the single sample of withdrawn fluid.
Claim 31
Dependent Claim
87 words
An electrode comprising: a non-corroding, conducting wire coated with an insulating polymer; a recess forming a channel in the insulating polymer coat, the recess bounded at one end by exposed wire and at an opposite end by the environment adjacent the coated wire; a multilayered polymeric composition within said channel, the composition comprising: a redox layer adjacent to and contacting said wire, the redox layer comprising a redox enzyme cross-linked to a redox polymer; and a biocompatible polymer layer adjacent to the environment outside the coated wire.
Claim 32
Dependent Claim
87 words
An electrode comprising: a non-corroding, conducting wire coated with an insulating polymer; a recess forming a channel in the insulating polymer coat, the recess bounded at one end by exposed wire and at an opposite end by the environment adjacent the coated wire; a multilayered polymeric composition within said channel, the composition comprising: a redox layer adjacent to and contacting said wire, the redox layer comprising a redox enzyme cross-linked to a redox polymer; and a biocompatible polymer layer adjacent to the environment outside the coated wire.
Claim 33
Dependent Claim
22 words
The electrode of claim 29, further comprising an analyte diffusion limiting barrier layer positioned between said redox layer and said biocompatible layer.
Claim 34
Independent Claim
144 words
A method for producing an in vivo biosensor to measure the concentration of an analyte, comprising the steps of: coating a non-corroding metal or carbon wire with a biocompatible insulating polymer containing less than 5% water when in equilibrium with physiological body fluids at 37° C. to form a coated wire; etching said coated wire to form a recess, said recess devoid of metal or carbon; immobilizing within said recess, adjacent to said wire, a redox composition comprising a redox enzyme and a redox polymer; and overcoating the contents of the recess with a biocompatible polymer; wherein said etched wire with said polymer overcoated recess and contents form a biosensor producing substantially no electrical signal output at zero concentration of the analyte to be measured, even in the presence of ascorbate, urate or acetaminophen in their normal concentration range in plasma, serum or blood.
Claim 35
Independent Claim
144 words
A method for producing an in vivo biosensor to measure the concentration of an analyte, comprising the steps of: coating a non-corroding metal or carbon wire with a biocompatible insulating polymer containing less than 5% water when in equilibrium with physiological body fluids at 37° C. to form a coated wire; etching said coated wire to form a recess, said recess devoid of metal or carbon; immobilizing within said recess, adjacent to said wire, a redox composition comprising a redox enzyme and a redox polymer; and overcoating the contents of the recess with a biocompatible polymer; wherein said etched wire with said polymer overcoated recess and contents form a biosensor producing substantially no electrical signal output at zero concentration of the analyte to be measured, even in the presence of ascorbate, urate or acetaminophen in their normal concentration range in plasma, serum or blood.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-01-14 | 2000-07-15 | 2001-01-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-01-14 | 2004-07-15 | 2005-01-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-01-14 | 2008-07-15 | 2009-01-14 | $7700.00 | Window Closed / Lapsed |