Patent US5578455A successfully refreshed from Google Patents.
US5578455A
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US
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Method for determining endotoxin and apparatus therefor
Assignee: Tanabe Pharma Corp
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Filing: 1994-03-14
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Granted: 1996-11-26
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Expires: 2014-03-14
Abstract
Abstract There is disclosed a method for determining an endotoxin by reacting the endotoxin adsorbed on an adsorbent having specific endotoxin adsorbability with an endotoxin detecting reagent, including the steps of: (a) preparing a column having a tip opening, a rear end opening and an adsorbent-filling and holding means provided in the column through which a sample solution can pass, (b) introducing the endotoxin adsorbent into the column through the tip opening to fill and hold the adsorbent therein, (c) introducing the sample solution into the column through the tip opening and discharging the introduced sample solution through the rear end opening to contact the sample and the adsorbent and thereby adsorbing the endotoxin on the adsorbent, and (d) reacting the detecting reagent with the endotoxin adsorbed on the adsorbent. There is also disclosed an apparatus for the above method.
Inventors
- 👤 Tetsuya Tosa
- 👤 Takeji Shibatani
- 👤 Taizo Watanabe
- 👤 Satoshi Minobe
- 👤 Makoto Masuda
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 06:31
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/579; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/81
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
153 words
A method for determining an endotoxin by reacting the endotoxin adsorbed on an adsorbent having specific endotoxin adsorbability with an endotoxin detecting reagent, which comprises the steps of: (a) preparing a column having a tip opening, a rear end opening and an adsorbent-filling and holding means provided in the column through which a sample solution can pass, (b) introducing the endotoxin adsorbent into the column through the tip opening to fill and hold the adsorbent therein, (c) introducing the sample solution into the column through the tip opening and discharging the introduced sample solution through the rear end opening to contact the sample and the adsorbent and thereby adsorbing the endotoxin on the adsorbent, (d) reacting the detecting reagent with the endotoxin adsorbed on the adsorbent, and (e) detecting and determining the endotoxin wherein steps (b) and (c) are carried out by imposing a negative pressure at the rear end of the column.
Claim 2
Independent Claim
153 words
A method for determining an endotoxin by reacting the endotoxin adsorbed on an adsorbent having specific endotoxin adsorbability with an endotoxin detecting reagent, which comprises the steps of: (a) preparing a column having a tip opening, a rear end opening and an adsorbent-filling and holding means provided in the column through which a sample solution can pass, (b) introducing the endotoxin adsorbent into the column through the tip opening to fill and hold the adsorbent therein, (c) introducing the sample solution into the column through the tip opening and discharging the introduced sample solution through the rear end opening to contact the sample and the adsorbent and thereby adsorbing the endotoxin on the adsorbent, (d) reacting the detecting reagent with the endotoxin adsorbed on the adsorbent, and (e) detecting and determining the endotoxin wherein steps (b) and (c) are carried out by imposing a negative pressure at the rear end of the column.
Claim 3
Dependent Claim
50 words
The method for determining an endotoxin according to claim 1, wherein the adsorbent-filling and holding means is a filter provided in an intermediate part of the interior of the column or a rear end part of the column, through which a sample solution can pass, while the adsorbent does not.
Claim 4
Dependent Claim
34 words
The method for determining an endotoxin according to claim 1, wherein step (d) is carried out by introducing the detecting reagent in the column by loading of negative pressure through the rear end opening.
Claim 5
Dependent Claim
26 words
The method for determining an endotoxin according to claim 1, wherein a negative pressure is loaded by suction through the rear end opening of the column.
Claim 6
Dependent Claim
37 words
The method for determining an endotoxin according to claim 1, wherein step (e) is conducted by discharging the adsorbent and the detecting reagent from the column through the tip opening of the column and then incubating it.
Claim 7
Dependent Claim
33 words
The method for determining an endotoxin according to claim 5, wherein discharge is carried out by stopping application of a negative pressure or applying pressure through the rear end opening of the column.
Claim 8
Dependent Claim
23 words
The method for determining an endotoxin according to claim 5, wherein the column is maintained in a perpendicular direction with the tip down.
Claim 9
Dependent Claim
32 words
The method for determining an endotoxin according to claim 5, wherein the determination of an endotoxin is carried out after a predetermined amount of a sample solution is introduced in the column.
Claim 10
Dependent Claim
18 words
The method for determining an endotoxin according to claim 5, wherein the detecting reagent is a Limulus reagent.
Claim 11
Dependent Claim
36 words
The method for determining an endotoxin according to claim 5, wherein steps (b) and (c) are carried out in one step by introducing the endotoxin adsorbent and the sample solution in the column after contacting them.
Claim 12
Dependent Claim
32 words
The method for determining an endotoxin according to claim 1, wherein the determination of an endotoxin is carried out after a predetermined amount of a sample solution is introduced in the column.
Claim 13
Dependent Claim
18 words
The method for determining an endotoxin according to claim 1, wherein the detecting reagent is a Limulus reagent.
Claim 14
Dependent Claim
37 words
The method for determining an endotoxin according to claim 1, wherein the steps (b) and (c) are carried out in one step by introducing the endotoxin adsorbent and the sample solution in the column after contacting them.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-11-26 | 2000-05-27 | 2000-11-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-11-26 | 2004-05-27 | 2004-11-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-11-26 | 2008-05-27 | 2008-11-26 | $7700.00 | Window Closed / Lapsed |