Surface-enhanced raman spectroscopy immunoassay method, composition and kit

Claim 1

A method for determining the presence or amount of an analyte in a test sample by monitoring an analyte-mediated ligand binding event in a test mixture, said method comprising: reacting the test sample with a conjugate of labeled specific binding member comprising a Raman-active label attached to a specific binding member specific for the analyte, under conditions permitting specific binding of the binding member to the analyte, if present, to give a first complex in the test mixture; adding an enhancer to the first complex in the test mixture; sequentially or simultaneously allowing the first complex to come into contact with a particulate having a surface capable of inducing a surface-enhanced Raman light scattering and having attached thereto a specific binding member specific for the analyte bound to the particulate through the specific binding member on a surface of the particulate to form a second complex, the particulate being characterized as having a surface capable of inducing a surface-enhanced Raman light scattering; illuminating the second complex with a radiation sufficient to cause the Raman-active labels in the complex to produce a detectable Raman spectrum; and then monitoring differences in the surface-enhanced Raman scattering spectra; the differences being dependent upon the amount of the analyte present in the test mixture.

Claim 2

A method for determining the presence or amount of an analyte in a test sample by monitoring an analyte-mediated ligand binding event in a test mixture, said method comprising: reacting the test sample with a conjugate of labeled specific binding member comprising a Raman-active label attached to a specific binding member specific for the analyte, under conditions permitting specific binding of the binding member to the analyte, if present, to give a first complex in the test mixture; adding an enhancer to the first complex in the test mixture; sequentially or simultaneously allowing the first complex to come into contact with a particulate having a surface capable of inducing a surface-enhanced Raman light scattering and having attached thereto a specific binding member specific for the analyte bound to the particulate through the specific binding member on a surface of the particulate to form a second complex, the particulate being characterized as having a surface capable of inducing a surface-enhanced Raman light scattering; illuminating the second complex with a radiation sufficient to cause the Raman-active labels in the complex to produce a detectable Raman spectrum; and then monitoring differences in the surface-enhanced Raman scattering spectra; the differences being dependent upon the amount of the analyte present in the test mixture.

Claim 3

A method for determining the presence or amount of an analyte, if any, in a test sample by monitoring an analyte-mediated ligand binding event, wherein the test sample analyte expresses more than one epitope recognized by one or more specific binding members, and wherein the one or more specific binding members are specific for the analyte or an ancillary specific binding member which is specific for the analyte or another specific binding member, the method comprising: forming a test mixture, in the presence or absence of an ancillary binding member, the mixture comprising the test sample; a Raman active label attached to the one or more specific binding members; and a particulate capture reagent, the particulate capture reagent comprising the one or more specific binding members attached to a particulate having a surface capable of inducing surface-enhanced Raman light scattering; separating the particulate from the test mixture by a porous material; illuminating the porous material with a radiation sufficient to cause the Raman-active label in the test mixture to emit a detectable Raman spectrum; and monitoring differences in spectral characteristics of detected surface-enhanced Raman scattering spectra, the differences being dependent upon the amount of the analyte present in the test mixture.

Claim 4

A method for determining the presence or amount of an analyte, if any, in a test sample by monitoring an analyte-mediated ligand binding event, wherein the test sample analyte expresses more than one epitope recognized by one or more specific binding members, and wherein the one or more specific binding members are specific for the analyte or an ancillary specific binding member which is specific for the analyte or another specific binding member, the method comprising: forming a test mixture, in the presence or absence of an ancillary binding member, the mixture comprising the test sample; a Raman active label attached to the one or more specific binding members; and a particulate capture reagent, the particulate capture reagent comprising the one or more specific binding members attached to a particulate having a surface capable of inducing surface-enhanced Raman light scattering; separating the particulate from the test mixture by a porous material; illuminating the porous material with a radiation sufficient to cause the Raman-active label in the test mixture to emit a detectable Raman spectrum; and monitoring differences in spectral characteristics of detected surface-enhanced Raman scattering spectra, the differences being dependent upon the amount of the analyte present in the test mixture.

Claim 7

A method for determining the presence or amount of an analyte, if any, in a test sample by monitoring an analyte-mediated ligand binding event, wherein the test sample analyte expresses at least one epitope recognized by one or more specific binding members, and wherein the one or more specific binding members arc specific for the analyte, analyte-analog, or an ancillary binding member which is specific for the analyte or another specific binding member, the method comprising: forming a test mixture, in the presence or absence of an ancillary binding member, the mixture comprising the test sample; a Raman active label attached to the one or more specific binding members; and a particulate capture reagent, the particulate capture reagent comprising analyte-analog attached to a particulate having a surface capable of inducing surface-enhanced Raman light scattering; allowing the analyte-analog to be bound to the one or more specific binding members, wherein the extent of the binding of the analyte-analog to the one or more specific binding members is affected by the presence of the test sample analyte; separating the particulate from the test mixture by a porous material: illuminating the porous material with a radiation sufficient to cause the Raman-active label in the test mixture to emit a detectable Raman spectrum: and monitoring differences in spectral characteristics of detected surface-enhanced Raman scattering spectra, the differences being dependent upon the amount of the test sample analyte present.

Claim 8

A method for determining the presence or amount of an analyte, if any, in a test sample by monitoring an analyte-mediated ligand binding event, wherein the test sample analyte expresses at least one epitope recognized by one or more specific binding members, and wherein the one or more specific binding members arc specific for the analyte, analyte-analog, or an ancillary binding member which is specific for the analyte or another specific binding member, the method comprising: forming a test mixture, in the presence or absence of an ancillary binding member, the mixture comprising the test sample; a Raman active label attached to the one or more specific binding members; and a particulate capture reagent, the particulate capture reagent comprising analyte-analog attached to a particulate having a surface capable of inducing surface-enhanced Raman light scattering; allowing the analyte-analog to be bound to the one or more specific binding members, wherein the extent of the binding of the analyte-analog to the one or more specific binding members is affected by the presence of the test sample analyte; separating the particulate from the test mixture by a porous material: illuminating the porous material with a radiation sufficient to cause the Raman-active label in the test mixture to emit a detectable Raman spectrum: and monitoring differences in spectral characteristics of detected surface-enhanced Raman scattering spectra, the differences being dependent upon the amount of the test sample analyte present.