US5550050A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for implanting encapsulated cells in a host
Assignee: Neurotech USA Inc
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Filing: 1994-04-15
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Granted: 1996-08-27
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Expires: 2014-04-15
Abstract
Abstract This invention provides methods for implanting encapsulated cells in a host comprising exposing cells to restrictive conditions for a sufficient period of time to establish a desired cell property in response to the restrictive conditions and implanting the encapsulated cells in a host, the cell property being substantially maintained following implantation. Also provided are cells produced by exposure to restrictive conditions.
Inventors
- 👤 Laura M. Holland
- 👤 Joseph P. Hammang
- 👤 Seth A. Rudnick
- 👤 Michael J. Lysaght
- 👤 Keith E. Dionne
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 05:35
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/0012; A61K9/0019; A61K9/0024; A61K35/00; A61K35/12; A61K35/13; A61K35/22; A61K9/0048; A61K9/0085; A61P; A61P27/00; A61P27/02; C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/48; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0613; C12N5/0614; C12N5/0618; A61K38/00; A61K48/00; A61K9/0087; A61K9/0092; C12N2500/00; C12N2500/02; C12N2500/30; C12N2500/34
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
65 words
A method for preparing encapsulated cells for implanting at an implantation site in a human comprising the steps of: (a) encapsulating the cells in a biocompatible capsule, and (b) exposing the cells to at least one restrictive condition that closely matches a condition at the implantation site and which establishes a measurable change in at least one cell property in response to the restrictive condition.
Claim 2
Independent Claim
65 words
A method for preparing encapsulated cells for implanting at an implantation site in a human comprising the steps of: (a) encapsulating the cells in a biocompatible capsule, and (b) exposing the cells to at least one restrictive condition that closely matches a condition at the implantation site and which establishes a measurable change in at least one cell property in response to the restrictive condition.
Claim 3
Dependent Claim
17 words
The method of claim 1 wherein the encapsulated cells are exposed to the restrictive condition in vitro.
Claim 4
Dependent Claim
36 words
The method of claim 1 wherein the encapsulated cells are exposed to the restrictive condition in vivo by implantation at an implantation site in a recipient prior to implantation at an implantation site in a human.
Claim 5
Dependent Claim
18 words
The method of claim 1 wherein the cells are xenogeneic to the host and the capsule is immunoisolatory.
Claim 6
Dependent Claim
24 words
The method of claim 1 wherein at least one restrictive condition is a glucose concentration ranging from about 40 mg/deciliter to about 70 mg/deciliter.
Claim 7
Dependent Claim
24 words
The method of claim 1 wherein at least one restrictive condition is an oxygen concentration ranging from about 40 mmHg to about 65 mmHg.
Claim 8
Dependent Claim
25 words
The method of claim 3 wherein the cells are selected from the group consisting of adrenal chromaffin cells, baby hamster kidney cells, and PC12 cells.
Claim 9
Dependent Claim
23 words
The method of claim 7 wherein the cells are adrenal chromaffin cells and are implanted in a recipient for 4 months or longer.
Claim 10
Dependent Claim
22 words
The method of claim 7 wherein the cells are PC12 cells and are implanted in a recipient for 6 months or longer.
Claim 11
Dependent Claim
24 words
The method of claim 1 wherein the cells produce a biologically active molecule selected from the group consisting of neurotransmitters, analgesics and growth factors.
Claim 12
Dependent Claim
28 words
The method of claim 1 wherein the capsule is implanted in a host at an implantation site selected from the group consisting of cerebrospinal fluid or brain parenchyma.
Claim 13
Dependent Claim
11 words
The method of claim 1 wherein the host is a human.
Claim 14
Independent Claim
78 words
A method for preparing encapsulated cells for implanting at an implantation site in a xenogeneic host comprising the steps of: (a) culturing the cells under at least two restrictive conditions, and (b) encapsulating the cells in a biocompatible capsule and further exposing the encapsulated cells to at least one restrictive condition that closely matches a condition at the implantation site and which establishes a measurable change in at least one cell property in response to the restrictive condition.
Claim 15
Independent Claim
78 words
A method for preparing encapsulated cells for implanting at an implantation site in a xenogeneic host comprising the steps of: (a) culturing the cells under at least two restrictive conditions, and (b) encapsulating the cells in a biocompatible capsule and further exposing the encapsulated cells to at least one restrictive condition that closely matches a condition at the implantation site and which establishes a measurable change in at least one cell property in response to the restrictive condition.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-08-27 | 2000-02-26 | 2000-08-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-08-27 | 2004-02-26 | 2004-08-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-08-27 | 2008-02-26 | 2008-08-27 | $7700.00 | Window Closed / Lapsed |