EP0078061A2
Expired / Lapsed
EP
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Method for culturing non-adhering mammalian cells
Assignee: Ajinomoto Co Inc
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Filing: 1982-10-27
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Granted: —
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Expires: 2002-10-27
Abstract
Abstract Non-adhering mammalian cells are cultured in an aqueous culture medium in suspension at a partial pressure of dissolved oxygen in the range from 0.01 bars (atm) to 0.05 bars (atm) until the middle stage of the exponential growth phase, and at a partial pressure of dissolved oxygen of more than 0.01 bars (atm) after the middle stage of exponential growth phase.
Inventors
- 👤 Hiroshiro Shibai
- 👤 Naoki Nishimura
- 👤 Tsuyoshi Nakamatsu
- 👤 Satoshi Takano
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 10:56
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0018; C12N2500/00; C12N2500/02
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
68 words
A method for culturing non-adhering mammalian cells, which comprises culturing non-adhering mammalian cells in an aqueous culture medium in suspension at a partial pressure of dissolved oxygen in the range from 0.01 bars (atm) to 0.05 bars (atm) until the middle stage of the exponential growth phase, and at a partial pressure of dissolved oxygen of more than 0.01 bars (atm) after the middle stage of.exponential growth phase.
Claim 2
Independent Claim
68 words
A method for culturing non-adhering mammalian cells, which comprises culturing non-adhering mammalian cells in an aqueous culture medium in suspension at a partial pressure of dissolved oxygen in the range from 0.01 bars (atm) to 0.05 bars (atm) until the middle stage of the exponential growth phase, and at a partial pressure of dissolved oxygen of more than 0.01 bars (atm) after the middle stage of.exponential growth phase.
Claim 3
Dependent Claim
37 words
The method of Claim 1, wherein said non-adhering mammalian cells are cultured at a partial pressure of dissolved oxygen in the range from 0.02 to 0.05 bars (atm) until the middle stage of the exponential growth phase.
Claim 4
Dependent Claim
35 words
The method of Claim 1 or Claim 2, wherein said non-adhering mammalian cells are cultured at a partial pressure of dissolved oxygen lower than 0.2 bars (atm) after the middle stage of exponential growth phase.
Claim 5
Dependent Claim
36 words
The method of Claim 1, 2 or 3, wherein said non-adhering mammalian cells are cultured at a partial pressure of dissolved oxygen higher than 0.02 bars (atm) after the middle stage of the exponential growth phase.
References Cited (Backward Citations)
No citation records indexed.
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