EP0188309A2
Expired / Lapsed
EP
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Microencapsulation of living cells
Assignee: Sanofi Pasteur Ltd
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Filing: 1986-01-03
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Granted: —
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Expires: 2006-01-03
Abstract
Abstract Living cellular material is microencapsulated in a biocompatible semi-permeable membrane formed by interaction of aqueous solutions of poly-anionic and -cationic acrylic polymers to form water-insoluble polymer-polymer complex. An aqueous suspension of the cellular material in the polyanionic or polycationic acrylic polymer is introduced in droplet form into an aqueous solution of the polycationic or polyanionic (respectively) acrylic polymer. Examples of living cellular material include hybridoma cells and Islets of Langerhans.
Inventors
- 👤 Anthony Mein-Fang Sun
- 👤 Hrire Marzakhan-Gharapetian
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 12:35
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/04; A; A61; A61F; A61F2/00; A61F2/02; A61F2/022; A61K; A61K9/00; A61K9/14; A61K9/16; A61K9/1605; A61K9/1629; A61K9/1635; A61K9/48; A61K9/50; A61K9/5005; A61K9/5021; A61K9/5026
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
53 words
A microcapsule comprising a biocompatible semi-permeable membrane surrounding a core containing living cellular material, characterized in that said membrane is formed by the ionic interaction of a matrix biocompatible, non-toxic acrylic polymer which is polyanionic or polycationic and a membrane biocompatible, non-toxic acrylic polymer which is oppositely charged to said matrix acrylic polymer.
Claim 2
Dependent Claim
53 words
A microcapsule comprising a biocompatible semi-permeable membrane surrounding a core containing living cellular material, characterized in that said membrane is formed by the ionic interaction of a matrix biocompatible, non-toxic acrylic polymer which is polyanionic or polycationic and a membrane biocompatible, non-toxic acrylic polymer which is oppositely charged to said matrix acrylic polymer.
Claim 3
Dependent Claim
23 words
A microcapsule as claimed in Claim 1, characterized in that said matrix acrylic polymer is polyanionic and said membrane acrylic polymer is polycationic.
Claim 4
Dependent Claim
18 words
A microcapsule as claimed in Claim 1 or Claim 2, having a diameter of 150 to 2000 micrometres.
Claim 5
Dependent Claim
28 words
A microcapsule as claimed in any one of Claims 1 to 3, characterized in that said membrane further comprises at least one additional layer of biocompatible polymeric material.
Claim 6
Dependent Claim
34 words
A microcapsule as claimed in Claim 4, characterized in that said at least one additional layer includes an outer biocompatible acrylic polymer material containing groups covalently bonded to a subsurface layer of said membrane.
Claim 7
Dependent Claim
53 words
A microcapsule as claimed in any one of Claims 1 to 5, characterized in that said matrix acrylic polymer is polyanionic and in that said membrane polymer is polycationic and contains (a) tertiary amine groups and carboxylic acid groups, (b) primary and/or secondary amine groups, or (c) epoxy groups and/or tertiary amine groups.
Claim 8
Dependent Claim
25 words
A microcapsule as claimed in any one of Claims 1 to 6, characterized in that said cellular material is hybridoma cells or Islets of Langerhans.
Claim 9
Independent Claim
100 words
A method for the formation of microcapsules, characterized by (a) forming an aqueous suspension of living cellular material in an aqueous solution of a first, water-soluble biocompatible, non-toxic acrylic polymer which is polyanionic or polycationic, and (b) introducing said aqueous suspension in droplet form to an aqueous solution of a second water-soluble, biocompatible, non-toxic acrylic polymer which is oppositely charged to said first acrylic polymer, so as to cause an interaction between the anionic and cationic groups at the interface between the suspension of living matter and the second acrylic polymer solution and the formation of a water-insoluble polymer-polymer complex.
Claim 10
Independent Claim
100 words
A method for the formation of microcapsules, characterized by (a) forming an aqueous suspension of living cellular material in an aqueous solution of a first, water-soluble biocompatible, non-toxic acrylic polymer which is polyanionic or polycationic, and (b) introducing said aqueous suspension in droplet form to an aqueous solution of a second water-soluble, biocompatible, non-toxic acrylic polymer which is oppositely charged to said first acrylic polymer, so as to cause an interaction between the anionic and cationic groups at the interface between the suspension of living matter and the second acrylic polymer solution and the formation of a water-insoluble polymer-polymer complex.
Claim 11
Dependent Claim
53 words
A method as claimed in Claim 8, characterized in that said microcapsules are reacted with an additional polymer containing groups reactive with surface groups of the microcapsule to increase the thickness of the microporous membrane wall and to provide sufficient strength to retain a spherical shape and structural integrity upon in vivo introduction.
Claim 12
Dependent Claim
39 words
A method as claimed in Claim 8 or Claim 9, characterized in that said aqueous suspension and said aqueous solution both have an approximately neutral pH and said first and second acrylic polymers are present in solubilized salt form.
References Cited (Backward Citations)
No citation records indexed.
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