US5543332A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Water-soluble, polymer-based reagents and conjugates comprising moieties derived from divinyl sulfone
Assignee: IMMUNODEX AS
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Filing: 1991-11-08
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Granted: 1996-08-06
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Expires: 2011-11-08
Abstract
Abstract Water soluble reagents are claimed, comprising a water-soluble polymeric carrier molecule having attached thereto more than one connecting moiety wherein the connecting moiety is derived from divinyl sulfone, and wherein each connecting moiety is attached to a reactive functional group on the polymeric molecule, and wherein the reagents are capable of reaction with a molecular species having a functional group which is reactive towards the terminal vinyl group of the more than one connecting moiety and the molecular species is selected from the group consisting of labelling species, marking species, and targeting species.
Inventors
- 👤 Allan O. F. Lihme
- 👤 Thomas Boenisch
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 22:32
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54353; Y; Y10; Y10S; Y10S436/00; Y10S436/823; Y10S436/828; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/813; Y10S530/814; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
149 words
A water-soluble reagent comprising a water-soluble polymeric carrier molecule having attached thereto more than one connecting moiety of the formula ##STR1## wherein (1) the connecting moiety is derived from divinyl sulfone, (2) each connecting moiety is attached to a reactive functional group on the polymeric molecule via a covalent linkage formed between one of the two vinyl groups of the connecting moiety and the reactive functional group on the polymeric molecule, (3) the content of free reactive terminal vinyl groups is in the range of from about 50 to about 5,000 μmoles of vinyl groups per gram of polymeric carrier, (4) the reagent is capable of reaction with a molecular species having a functional group which is reactive towards the terminal vinyl group of the more than one connecting moiety, and (5) the molecular species is selected from the group consisting of labelling species, marking species, and targeting species.
Claim 2
Dependent Claim
149 words
A water-soluble reagent comprising a water-soluble polymeric carrier molecule having attached thereto more than one connecting moiety of the formula ##STR1## wherein (1) the connecting moiety is derived from divinyl sulfone, (2) each connecting moiety is attached to a reactive functional group on the polymeric molecule via a covalent linkage formed between one of the two vinyl groups of the connecting moiety and the reactive functional group on the polymeric molecule, (3) the content of free reactive terminal vinyl groups is in the range of from about 50 to about 5,000 μmoles of vinyl groups per gram of polymeric carrier, (4) the reagent is capable of reaction with a molecular species having a functional group which is reactive towards the terminal vinyl group of the more than one connecting moiety, and (5) the molecular species is selected from the group consisting of labelling species, marking species, and targeting species.
Claim 3
Dependent Claim
37 words
A reagent according to claim 1, wherein the polymeric carrier is selected from the group consisting of natural and synthetic polysaccharides, homopoly amino acids, natural and synthetic polypeptides and proteins, and synthetic polymers having nucleophilic functional groups.
Claim 4
Dependent Claim
26 words
A reagent according to claim 1, wherein the polymeric carrier is selected from the group consisting of polyvinyl alcohols, polyallyl alcohols, polyethylene glycols and substituted polyacrylates.
Claim 5
Dependent Claim
30 words
A reagent according to claim 1, wherein the polymeric carrier is selected from the group consisting of dextrans, carboxymethyl-dextrans, starches, hydroxyethyl-starches, hydroxypropyl-starches, glycogen, agarose derivatives, cellulose derivatives and natural gums.
Claim 6
Dependent Claim
20 words
A reagent according to claim 1, wherein the polymeric carrier is selected from the group consisting of hydroxyethyl-celluloses and hydroxypropyl-celluloses.
Claim 7
Dependent Claim
71 words
A water-soluble conjugate obtained by reaction of a water-soluble reagent according to claim 1 with a molecular species which is different from the polymeric carrier molecule wherein the molecular species is selected from the group consisting of labelling species, marking species and targeting species, and wherein the conjugate has more than one molecule covalently attached thereto each via a connecting group derived from a terminal vinyl group of the water-soluble reagent.
Claim 8
Dependent Claim
28 words
A conjugate according to claim 6 having a content of from 2 to 500 of remaining free reactive terminal vinyl groups capable of reaction with a molecular species.
Claim 9
Dependent Claim
46 words
A conjugate according to claims 6 or 7, wherein the molecular species is selected from the group consisting of proteins; enzymes; toxins; drugs; dyes; fluorescent, luminescent, phosphorescent and other light-emitting substances; metal-chelating substances; substances labelled with a radioactive isotope; and substances labelled with a heavy atom.
Claim 10
Dependent Claim
40 words
A conjugate according to claims 6 or 7, wherein the molecular species is selected from the group consisting of ferritin, phycoerythrins, phycocyanins, phycobilins, horseradish peroxidase, alkaline phosphatase, glucose oxidases, galactosidases, ureases, iminodiacetic acid, ethylenediaminetetraacetic acid, diethylenetriaminepentaacetic acid, and desferrioxamine B.
Claim 11
Dependent Claim
58 words
A conjugate according to claims 6 or 7, wherein the molecular species is a targeting species selected from the group consisting of: antigens; haptens; monoclonal and polyclonal antibodies; gene probes; natural and synthetic oligo- and polynucleotides; natural and synthetic mono-, oligo- and polysaccharides; lectins; avidin and streptavidin; biotin; growth factors; hormones; receptor molecules; protein A and protein G.
Claim 12
Dependent Claim
22 words
A conjugate according to claims 6 or 7, wherein the molecular species of the conjugate are of at least two different types.
Claim 13
Dependent Claim
37 words
A conjugate according to claim 6, wherein the polymeric carrier is selected from the group consisting of natural and synthetic polysaccharides; homopoly amino acids; natural and synthetic polypeptides and proteins; and synthetic polymers having nucleophilic functional groups.
Claim 14
Dependent Claim
26 words
A conjugate according to claim 6, wherein the polymeric carrier is selected from the group consisting of polyvinyl alcohols, polyallyl alcohols, polyethylene glycols and substituted polyacrylates.
Claim 15
Dependent Claim
30 words
A conjugate according to claim 6, wherein the polymeric carrier is selected from the group consisting of dextrans, carboxymethyl-dextrans, starches, hydroxyethyl-starches, hydroxypropyl-starches, glycogen, agarose derivatives, cellulose derivatives and natural gums.
Claim 16
Dependent Claim
20 words
A conjugate according to claim 1, wherein the polymeric carrier is selected from the group consisting of hydroxyethyl-celluloses and hydroxypropyl-celluloses.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1999-08-06 | 2000-02-05 | 2000-08-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2003-08-06 | 2004-02-05 | 2004-08-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2007-08-06 | 2008-02-05 | 2008-08-06 | $7700.00 | Window Closed / Lapsed |